FastQCFastQC Report
Thu 26 May 2016
SRR805314_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805314_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13433958
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3011502.241707172227277No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG179150.13335608165516075No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA144220.10735480935700409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA973550.043.97786713
TCAACGC973550.043.97545212
ATCAACG979150.043.69034211
AACGCAG981200.043.60624714
GTGGTAT973750.043.592356
ACGCAGA980800.043.55454615
GGTATCA976600.043.5541658
AGTGGTA974700.043.47035
GTATCAA979750.043.4285289
TGGTATC978550.043.397737
CGCAGAG989250.043.14687716
AAGCAGT996750.043.069781
AGAGTAC961800.043.0491319
TATCAAC990050.042.9719710
CAGTGGT1000450.042.4195634
GCAGTGG1011550.042.3234633
CAGAGTA984450.042.2329218
AGCAGTG1018750.042.098162
GAGTACT475300.042.04586420
GCAGAGT996700.041.88361717