##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805314_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13433958
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.784415062187925	35.0	33.0	35.0	32.0	37.0
2	34.59405076299926	35.0	35.0	37.0	32.0	37.0
3	36.82616850521641	39.0	37.0	39.0	33.0	39.0
4	36.84558303665978	39.0	37.0	39.0	33.0	39.0
5	36.891877732534226	39.0	37.0	39.0	33.0	39.0
6	36.97194118070043	39.0	37.0	39.0	33.0	39.0
7	36.800422183841874	39.0	37.0	39.0	33.0	39.0
8	37.695239109724774	40.0	37.0	40.0	33.0	40.0
9	37.70911193856643	40.0	37.0	40.0	33.0	40.0
10	37.35211432103629	39.0	37.0	40.0	32.0	40.0
11	37.463042984055775	39.0	37.0	40.0	33.0	40.0
12	37.4768325909609	39.0	37.0	40.0	33.0	40.0
13	37.58721189987344	40.0	37.0	40.0	33.0	40.0
14	37.542592436272315	40.0	37.0	40.0	32.0	40.0
15	37.45779315373771	39.0	37.0	40.0	32.0	40.0
16	37.312468819688135	39.0	37.0	40.0	32.0	40.0
17	37.19941033014991	39.0	36.0	40.0	32.0	40.0
18	37.14341514243233	39.0	36.0	40.0	32.0	40.0
19	37.05829488226776	39.0	36.0	40.0	32.0	40.0
20	36.92498688770651	39.0	36.0	40.0	31.0	40.0
21	36.71875064668209	39.0	36.0	40.0	31.0	40.0
22	36.6979529041255	39.0	36.0	40.0	30.0	40.0
23	36.310069973421086	38.0	35.0	40.0	30.0	40.0
24	36.407926465156436	38.0	35.0	40.0	30.0	40.0
25	36.41908862600285	38.0	36.0	40.0	30.0	40.0
26	36.32841572081735	38.0	35.0	40.0	30.0	40.0
27	36.30699805671568	38.0	35.0	40.0	30.0	40.0
28	36.15329845455822	38.0	35.0	40.0	30.0	40.0
29	36.09804720247004	38.0	35.0	40.0	30.0	40.0
30	35.97628561887718	38.0	35.0	40.0	29.0	40.0
31	35.82981635047541	38.0	35.0	40.0	29.0	40.0
32	35.67629607000409	38.0	34.0	40.0	28.0	40.0
33	35.53067041001617	38.0	34.0	40.0	27.0	40.0
34	35.44106100376374	38.0	34.0	40.0	27.0	40.0
35	35.29441018052908	38.0	34.0	40.0	27.0	40.0
36	35.16327503778112	38.0	34.0	40.0	27.0	40.0
37	34.992834650815496	38.0	33.0	40.0	26.0	40.0
38	34.91755832495531	38.0	33.0	40.0	26.0	40.0
39	34.82725567550531	37.0	33.0	40.0	26.0	40.0
40	35.07541239893708	38.0	33.0	40.0	27.0	40.0
41	35.160469535486115	38.0	33.0	40.0	27.0	40.0
42	35.14516927922508	38.0	33.0	40.0	27.0	40.0
43	35.114547328493956	38.0	33.0	40.0	27.0	40.0
44	34.99314557928497	38.0	33.0	40.0	26.0	40.0
45	34.84745672124328	37.0	33.0	40.0	26.0	40.0
46	34.737333033198404	37.0	33.0	40.0	26.0	40.0
47	34.620265673005676	37.0	33.0	40.0	26.0	40.0
48	34.55864191327679	37.0	33.0	40.0	26.0	40.0
49	34.33644976409782	37.0	33.0	40.0	25.0	40.0
50	34.15809138304586	37.0	32.0	39.0	25.0	40.0
51	33.926557162081345	36.0	32.0	39.0	24.0	40.0
52	33.64718238660564	36.0	32.0	39.0	23.0	40.0
53	33.41218314066487	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	11.0
12	35.0
13	74.0
14	239.0
15	612.0
16	1441.0
17	3037.0
18	5935.0
19	11009.0
20	18381.0
21	28122.0
22	41437.0
23	58272.0
24	78139.0
25	104289.0
26	133733.0
27	169435.0
28	212156.0
29	263273.0
30	325517.0
31	401167.0
32	501486.0
33	633240.0
34	821421.0
35	1098180.0
36	1516378.0
37	2148330.0
38	2843668.0
39	2014940.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.101978731807856	45.1345463488869	31.39707597716176	14.366398942143485
2	30.23748473830274	44.490097408373614	14.378934339380844	10.893483513942801
3	36.59801526847114	25.6013678172881	21.401138815530018	16.399478098710745
4	22.62250633804274	26.693532911149493	20.377516440054375	30.30644431075339
5	19.045369949794395	35.167573100943144	24.71295503529191	21.07410191397055
6	28.40782292158424	25.025580696321963	22.839776631726853	23.726819750366943
7	22.23264357384473	24.75902485328598	31.035261536473463	21.973070036395825
8	26.136749869249254	26.196754523127137	25.94213857152151	21.72435703610209
9	28.10171804914084	24.928892884732853	25.516128604838574	21.453260461287734
10	21.58975039225223	26.30325329288658	31.12766170625217	20.97933460860902
11	20.92999695249903	32.83255761258149	25.347764225554375	20.8896812093651
12	21.05541791927591	25.813397659870606	31.849742272530552	21.28144214832293
13	21.02725793842738	26.169837660650717	25.15336879868167	27.64953560224023
14	21.256356466203037	32.81819847881019	24.832688921611933	21.09275613337484
15	21.251644526505146	32.526266644573404	24.711473714596995	21.51061511432446
16	21.417492893754766	25.786101162442222	24.879026717219155	27.917379226583854
17	28.16356132719784	25.495598542142233	24.98697703238316	21.353863098276772
18	21.58163662563185	25.263060968331153	24.84048260386105	28.31481980217595
19	21.48188195913669	32.46446058562935	24.668381425637925	21.385276029596042
20	28.456453414548417	25.801971392198787	24.747762349711085	20.99381284354172
21	22.024253760507513	31.96865733836595	24.60510148982154	21.401987411304994
22	28.177518494549407	25.74839075721392	24.867957753031533	21.206132995205138
23	21.819630521399574	25.498315537386674	31.571916482097084	21.110137459116665
24	21.905539677881976	31.69955570800504	24.792373178478	21.60253143563498
25	21.47364164753232	25.781887958857695	24.66896204379975	28.075508349810235
26	21.683877528871236	28.908241338851887	28.066642757108518	21.341238375168363
27	21.92337507680164	25.10170122610179	31.395512774418382	21.579410922678186
28	25.098567376792452	25.446566082758338	28.08310104884949	21.37176549159972
29	25.378670976937695	25.42676551467557	27.89932051298657	21.295242995400166
30	25.528291810946556	25.03799699239792	27.875083426641652	21.55862777001387
31	24.258457559566583	25.85465132465056	28.16326357429434	21.723627541488515
32	23.499403526496064	26.297930959736515	28.349158155772113	21.853507357995312
33	23.49060492819763	25.708156896128454	28.39046392731018	22.410774248363737
34	22.84083365453428	26.12652950083661	28.574527328431426	22.458109516197684
35	22.714444990821022	26.14415647272382	28.83442839407418	22.306970142380973
36	23.059562937445538	25.82295552807296	28.78648273278806	22.33099880169344
37	23.1239296713597	26.072286365641457	28.62029194969941	22.18349201329943
38	23.121443434615472	26.11483525555164	28.786251974287847	21.97746933554504
39	23.253035330317395	25.821920836733298	28.67125980295606	22.253784029993245
40	22.709003556509554	26.46852848579696	28.533162006312658	22.289305951380822
41	22.93500545408881	26.4483110636493	28.531301050665782	22.08538243159611
42	23.112324751945778	26.088253365091656	28.496962697069623	22.302459185892946
43	22.805840244550417	26.48876823941239	28.73339339009397	21.971998125943227
44	23.032110119742818	26.175011117349033	28.68220222215969	22.110676540748454
45	23.282029019295727	25.72036476517196	28.540896137981075	22.456710077551232
46	22.882675381298647	26.085246060766305	28.461299343052882	22.570779214882165
47	22.823028030904965	26.122070651106693	28.605024669572437	22.449876648415902
48	22.917207274282084	25.698569252635746	28.617999252342457	22.766224220739712
49	22.632518279422936	26.373999382758228	28.41878022843305	22.574702109385782
50	23.1068312108762	26.287055534936165	28.387359853291187	22.218753400896446
51	23.21392548644264	25.848472951902927	28.373573893859128	22.5640276677953
52	23.162994852298926	26.224140346426573	28.17620838177401	22.43665641950049
53	23.352291260699193	26.260027015120933	27.980636830932475	22.407044893247395
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16891.0
1	9868.0
2	2845.0
3	2352.0
4	1859.0
5	1908.5
6	1958.0
7	2103.0
8	2712.5
9	3177.0
10	4249.0
11	5321.0
12	7281.0
13	9241.0
14	11488.5
15	13736.0
16	18937.0
17	24138.0
18	32830.5
19	41523.0
20	56387.5
21	71252.0
22	256954.0
23	442656.0
24	322108.5
25	210679.5
26	219798.0
27	244618.5
28	269439.0
29	292344.5
30	315250.0
31	333326.5
32	351403.0
33	383353.0
34	415303.0
35	460917.0
36	506531.0
37	549458.0
38	592385.0
39	629843.0
40	667301.0
41	705431.5
42	774111.5
43	804661.0
44	837446.0
45	870231.0
46	885581.0
47	900931.0
48	909012.0
49	917093.0
50	912854.5
51	908616.0
52	884805.0
53	860994.0
54	822796.5
55	784599.0
56	743949.0
57	703299.0
58	628466.0
59	481808.0
60	409983.0
61	353364.0
62	296745.0
63	250756.5
64	204768.0
65	167843.5
66	130919.0
67	104286.5
68	77654.0
69	60301.0
70	42948.0
71	32754.0
72	22560.0
73	17122.0
74	11684.0
75	8844.0
76	4634.5
77	3265.0
78	2617.0
79	1969.0
80	1527.0
81	1085.0
82	764.5
83	444.0
84	330.0
85	216.0
86	170.5
87	125.0
88	92.5
89	60.0
90	53.5
91	47.0
92	35.5
93	17.5
94	11.0
95	9.5
96	8.0
97	6.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.3433958E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.71696556421126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.36936488898783	37.21071552542846
2	14.27530631420671	14.480004375123787
3	4.951752875093652	7.534136401458265
4	2.3067459342710315	4.67964616455233
5	1.2521131476664438	3.17516896963476
6	0.8051400844523218	2.4500557162520726
7	0.55181487752556	1.9590463298898302
8	0.4113527188700977	1.6690049342143538
9	0.3024076977629875	1.3803480714418055
>10	1.6298911996406116	14.843592117692388
>50	0.10392377309305155	3.583565630065919
>100	0.03852754717186088	3.2091799276888553
>500	0.0010726015872751461	0.39019688311295375
>1k	4.360016016629322E-4	0.4052487584445227
>5k	7.516903449750058E-5	0.29250600810452065
>10k+	7.516903449750058E-5	2.7375841868952717
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	301150	2.241707172227277	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	17915	0.13335608165516075	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14422	0.10735480935700409	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1165733881258226E-4	0.0	0.0	0.0	0.0
2	0.001124017210713328	0.0	0.0	0.0	0.0
3	0.0011314610333008335	0.0	0.0	0.0	0.0
4	0.001369663356101009	0.0	0.0	0.0	0.0
5	0.001369663356101009	0.0	0.0	0.0	7.443822587505484E-6
6	0.0013771071786885145	0.0	0.0	0.0	2.233146776251645E-5
7	0.0013771071786885145	0.0	0.0	0.0	2.233146776251645E-5
8	0.00138455100127602	0.0	0.0	0.0	3.7219112937527423E-5
9	0.0014217701142135474	0.0	0.0	0.0	3.7219112937527423E-5
10	0.0014441015819760638	0.0	0.0	0.0	4.46629355250329E-5
11	0.001488764517501097	0.0	0.0	0.0	5.9550580700043875E-5
12	0.0015185398078511189	0.0	0.0	0.0	5.9550580700043875E-5
13	0.001920506227576415	0.0	0.0	0.0	7.443822587505485E-5
14	0.0021140456148515574	0.0	0.0	0.0	8.188204846256033E-5
15	0.002411798518351777	0.0	0.0	0.0	8.188204846256033E-5
16	0.0025755626152768976	0.0	0.0	0.0	8.93258710500658E-5
17	0.002724439067027007	0.0	0.0	0.0	8.93258710500658E-5
18	0.002970085212414688	0.0	0.0	0.0	8.93258710500658E-5
19	0.0031115178415772925	0.0	0.0	0.0	1.1165733881258226E-4
20	0.003349720164377468	0.0	0.0	0.0	1.2654498398759323E-4
21	0.003535815729065105	0.0	0.0	0.0	1.2654498398759323E-4
22	0.0037070236485777313	0.0	0.0	0.0	1.339888065750987E-4
23	0.004041995665015478	0.0	0.0	0.0	1.488764517501097E-4
24	0.004265310342640643	0.0	0.0	0.0	1.488764517501097E-4
25	0.004458849729915785	0.0	0.0	0.0	1.488764517501097E-4
26	0.004652389117190928	0.0	0.0	0.0	1.5632027433761517E-4
27	0.005054355536916224	0.0	0.0	0.0	1.5632027433761517E-4
28	0.0052627825693663775	0.0	0.0	0.0	1.786517421001316E-4
29	0.005955058070004388	0.0	0.0	0.0	1.8609556468763712E-4
30	0.0063049177316171455	0.0	0.0	0.0	1.8609556468763712E-4
31	0.006825985312742529	0.0	0.0	0.0	1.8609556468763712E-4
32	0.007287502313167869	0.0	0.0	0.0	1.8609556468763712E-4
33	0.007607586684430605	0.0	0.0	0.0	2.0098320986264808E-4
34	0.007927671055693341	0.0	0.0	0.0	2.0098320986264808E-4
35	0.008299862185068615	0.0	0.0	0.0	2.1587085503765903E-4
36	0.008753935362906449	0.0	0.0	0.0	2.3075850021267002E-4
37	0.009215452363331789	0.0	0.0	0.0	2.382023228001755E-4
38	0.009729076121869669	0.0	0.0	0.0	2.45646145387681E-4
39	0.010190593122295009	0.0	0.0	0.0	2.45646145387681E-4
40	0.010585115719432799	0.0	0.0	0.0	2.5308996797518646E-4
41	0.010942419203633062	0.0	0.0	0.0	2.679776131501974E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGCA	97355	0.0	43.977867	13
TCAACGC	97355	0.0	43.975452	12
ATCAACG	97915	0.0	43.690342	11
AACGCAG	98120	0.0	43.606247	14
GTGGTAT	97375	0.0	43.59235	6
ACGCAGA	98080	0.0	43.554546	15
GGTATCA	97660	0.0	43.554165	8
AGTGGTA	97470	0.0	43.4703	5
GTATCAA	97975	0.0	43.428528	9
TGGTATC	97855	0.0	43.39773	7
CGCAGAG	98925	0.0	43.146877	16
AAGCAGT	99675	0.0	43.06978	1
AGAGTAC	96180	0.0	43.04913	19
TATCAAC	99005	0.0	42.97197	10
CAGTGGT	100045	0.0	42.419563	4
GCAGTGG	101155	0.0	42.323463	3
CAGAGTA	98445	0.0	42.23292	18
AGCAGTG	101875	0.0	42.09816	2
GAGTACT	47530	0.0	42.045864	20
GCAGAGT	99670	0.0	41.883617	17
>>END_MODULE