FastQCFastQC Report
Thu 26 May 2016
SRR805313_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805313_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12382840
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1647511.3304783070765671No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA377060.3045020366894832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC522050.042.76410312
CAACGCA523000.042.64598513
AACGCAG526500.042.30446214
ATCAACG528550.042.2293111
ACGCAGA526250.042.1950615
GTGGTAT524450.042.1561626
AAGCAGT526450.042.0585061
GGTATCA529500.041.896138
GTATCAA531600.041.7350469
TGGTATC529550.041.7324147
AGTGGTA529200.041.6978455
CGCAGAG535000.041.4873816
TATCAAC536650.041.3379310
AGAGTAC522200.041.1677519
CAGAGTA538350.040.1204618
AGCAGTG555700.039.870072
CAGTGGT553050.039.8146634
GCAGAGT545600.039.75531417
GCAGTGG557750.039.710893
GAGTACT273450.039.5834720
Produced by FastQC (version 0.11.5)