##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805313_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12382840
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.299664212733106	35.0	35.0	37.0	32.0	37.0
2	34.76369362763308	35.0	35.0	37.0	32.0	37.0
3	36.63464181076393	38.0	35.0	39.0	32.0	39.0
4	36.7462448032923	39.0	37.0	39.0	33.0	39.0
5	36.84364758003818	39.0	37.0	39.0	33.0	39.0
6	36.82916083870905	39.0	37.0	39.0	33.0	39.0
7	36.73956725597682	39.0	37.0	39.0	33.0	39.0
8	37.600135833136825	40.0	37.0	40.0	33.0	40.0
9	37.598753597720716	40.0	37.0	40.0	33.0	40.0
10	37.39797267831935	40.0	37.0	40.0	32.0	40.0
11	37.42258561040924	40.0	37.0	40.0	32.0	40.0
12	37.41829661047062	39.0	37.0	40.0	32.0	40.0
13	37.52964869125338	40.0	37.0	40.0	32.0	40.0
14	37.41829047294482	40.0	37.0	40.0	32.0	40.0
15	37.31330914394436	39.0	37.0	40.0	32.0	40.0
16	37.240447021846364	39.0	36.0	40.0	32.0	40.0
17	37.11749461351354	39.0	36.0	40.0	32.0	40.0
18	37.06282985163339	39.0	36.0	40.0	32.0	40.0
19	36.96176660604514	39.0	36.0	40.0	31.0	40.0
20	36.76714453227208	39.0	36.0	40.0	31.0	40.0
21	36.634292294820895	39.0	36.0	40.0	30.0	40.0
22	36.57702958287437	39.0	36.0	40.0	30.0	40.0
23	36.33501636135168	39.0	36.0	40.0	30.0	40.0
24	36.39286294581857	39.0	36.0	40.0	30.0	40.0
25	36.365623798740835	39.0	36.0	40.0	30.0	40.0
26	36.232457497633824	38.0	35.0	40.0	30.0	40.0
27	36.17555835333413	38.0	35.0	40.0	30.0	40.0
28	36.05518766292708	38.0	35.0	40.0	30.0	40.0
29	35.96151448294575	38.0	35.0	40.0	29.0	40.0
30	35.8642983354384	38.0	35.0	40.0	29.0	40.0
31	35.717426616188206	38.0	35.0	40.0	28.0	40.0
32	35.53589750008883	38.0	34.0	40.0	27.0	40.0
33	35.44859692929893	38.0	34.0	40.0	27.0	40.0
34	35.35669943244038	38.0	34.0	40.0	27.0	40.0
35	35.240502986390844	38.0	34.0	40.0	26.0	40.0
36	35.16481526047337	38.0	34.0	40.0	26.0	40.0
37	35.04686227069073	38.0	33.0	40.0	26.0	40.0
38	34.96143881371317	38.0	33.0	40.0	26.0	40.0
39	34.86184930112963	38.0	33.0	40.0	26.0	40.0
40	34.97538270703651	38.0	33.0	40.0	26.0	40.0
41	35.020865165018684	38.0	33.0	40.0	26.0	40.0
42	34.94253943360328	38.0	33.0	40.0	26.0	40.0
43	34.92945140210162	38.0	33.0	40.0	26.0	40.0
44	34.81140861062567	38.0	33.0	40.0	26.0	40.0
45	34.62709887231039	37.0	33.0	40.0	25.0	40.0
46	34.506641448972935	37.0	33.0	40.0	24.0	40.0
47	34.441256690710695	37.0	33.0	40.0	24.0	40.0
48	34.35921113411786	37.0	33.0	40.0	24.0	40.0
49	34.16385376860236	37.0	33.0	40.0	24.0	40.0
50	34.002566616382026	37.0	32.0	39.0	24.0	40.0
51	33.82152034589803	36.0	32.0	39.0	23.0	40.0
52	33.56995035064654	36.0	31.0	39.0	23.0	40.0
53	33.36714097896767	36.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	13.0
10	46.0
11	71.0
12	207.0
13	415.0
14	792.0
15	1575.0
16	2799.0
17	4677.0
18	7780.0
19	13115.0
20	20857.0
21	31585.0
22	45954.0
23	62989.0
24	83979.0
25	108780.0
26	138716.0
27	171999.0
28	211875.0
29	259812.0
30	315978.0
31	382858.0
32	467003.0
33	580202.0
34	734834.0
35	958262.0
36	1294763.0
37	1814347.0
38	2550974.0
39	2115578.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.106201808308917	31.54731870879378	17.249871596499673	38.09660788639763
2	22.248143398444945	27.602230183059785	28.40589880835091	21.74372761014436
3	19.709485061585223	23.965560404559856	30.47219377784095	25.852760756013964
4	17.583575334898942	26.23522552177045	29.795160076363743	26.386039066966866
5	22.136521185769986	29.84434103969687	28.22186186690614	19.797275907627007
6	24.603596590119874	25.252874138727467	28.702535121183832	21.44099414996883
7	22.812117414098864	26.440113899557776	30.14805973427744	20.599708952065924
8	23.93152943912705	24.893223202431752	28.50924343688524	22.666003921555962
9	24.19959395421406	24.33412690465192	29.52051387242345	21.94576526871057
10	21.826123894034	25.3914287837039	30.362719699196628	22.41972762306547
11	21.488487293706452	29.64643005966321	26.55226910789447	22.312813538735863
12	21.575123315814466	25.78584557339027	30.191716924388913	22.44731418640635
13	21.992943460466257	26.01273213576207	25.773828943925626	26.220495459846045
14	22.264520901505634	29.921899984171645	25.589800078172697	22.223779036150027
15	21.956869344996786	29.801023028642863	25.798177154836853	22.443930471523498
16	22.345544317781705	25.9706900840195	25.44171611681973	26.24204948137907
17	26.384843864573877	25.77705114497159	25.483475519347742	22.35462947110679
18	22.34916222772805	25.727143369372452	25.44593162796257	26.47776277493693
19	22.674394565382418	29.397076922579956	25.25846251748387	22.670065994553752
20	26.366746239150306	25.88033116797116	25.287244283217742	22.465678309660788
21	22.702336459164457	29.418420976125027	25.47659502989621	22.402647534814307
22	26.27759867687865	25.536912372282934	25.488813551656968	22.696675399181448
23	22.488169111447778	25.78925351534866	29.385415623556472	22.33716174964709
24	22.34855655083971	29.409723456008475	25.65038391839029	22.591336074761525
25	22.390364407518792	25.612185895965705	25.51364630407887	26.483803392436627
26	22.483323696341063	27.481748936431384	27.65250136479192	22.38242600243563
27	22.485269938075593	25.45717299101014	29.533871066734285	22.52368600417998
28	24.521482955444792	25.473332450391023	27.315090883836017	22.690093710328163
29	24.58809933747024	25.585891443319948	27.327107513300664	22.498901705909145
30	24.400355653468832	25.445632827364324	27.458071007943254	22.695940511223597
31	24.000245501032076	25.60578994802485	27.33367305077026	23.06029150017282
32	23.72824004832494	26.156608661664045	27.38278133287679	22.732369957134228
33	23.465473187087937	25.9818103116894	27.486699335532077	23.066017165690585
34	23.383545293325277	25.91130952188674	27.412822906538402	23.29232227824958
35	23.303587868372684	26.177928488133578	27.521360204928758	22.99712343856498
36	23.292613003155978	26.020299059020385	27.614367947902096	23.072719989921538
37	23.508936560595146	25.935770792483794	27.322076357281528	23.233216289639532
38	23.617627297130543	26.148427985825546	27.483339847724753	22.750604869319154
39	23.558836260502435	25.998736961795515	27.546887466849284	22.89553931085276
40	23.381429462062016	26.092301927506128	27.36410225764041	23.162166352791445
41	23.463510793969718	26.322507599226025	27.465742915195545	22.74823869160871
42	23.280523692464733	26.075124930952835	27.696384674275045	22.947966702307387
43	23.294712683035556	25.985856233303505	27.56827997454542	23.151151109115517
44	23.425668101986297	26.05495185272522	27.586571416573257	22.93280862871522
45	23.431046512754747	25.782558766809554	27.68206647263471	23.10432824780099
46	23.34461238294285	25.847737675686677	27.352545942610902	23.455103998759576
47	23.31017763291781	26.022778296416654	27.502455010320737	23.164589060344802
48	23.17872152107271	25.853705611959775	27.630099395615222	23.33747347135229
49	23.317219636206236	26.111998539914914	27.198550574827745	23.372231249051108
50	23.516850738602777	26.23063045311092	27.335562762661876	22.916956045624428
51	23.45431258095881	25.97304011034625	27.425388683048478	23.147258625646458
52	23.629595472444123	25.987535977207166	27.003877947223735	23.37899060312497
53	23.652433528980428	26.200015505328338	27.016104544676345	23.13144642101489
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39672.0
1	23023.0
2	6374.0
3	5136.5
4	3899.0
5	3794.5
6	3690.0
7	4014.0
8	4911.0
9	5484.0
10	6815.0
11	8146.0
12	10727.5
13	13309.0
14	15678.5
15	18048.0
16	23047.5
17	28047.0
18	36083.5
19	44120.0
20	57598.5
21	71077.0
22	178273.0
23	285469.0
24	223956.5
25	172168.5
26	181893.0
27	198839.0
28	215785.0
29	236688.5
30	257592.0
31	279326.0
32	301060.0
33	329965.0
34	358870.0
35	389482.5
36	420095.0
37	455295.0
38	490495.0
39	523981.0
40	557467.0
41	593411.0
42	666175.5
43	702996.0
44	739261.5
45	775527.0
46	803993.0
47	832459.0
48	845866.0
49	859273.0
50	863187.0
51	867101.0
52	855606.0
53	844111.0
54	813039.0
55	781967.0
56	738188.0
57	694409.0
58	632460.5
59	505750.0
60	440988.0
61	385781.5
62	330575.0
63	281997.0
64	233419.0
65	190415.5
66	147412.0
67	118428.5
68	89445.0
69	69829.5
70	50214.0
71	38994.5
72	27775.0
73	20474.5
74	13174.0
75	9906.0
76	5088.5
77	3539.0
78	2719.0
79	1899.0
80	1605.5
81	1312.0
82	1028.0
83	744.0
84	522.0
85	300.0
86	223.0
87	146.0
88	112.0
89	78.0
90	63.5
91	49.0
92	36.5
93	18.0
94	12.0
95	10.0
96	8.0
97	5.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.238284E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.16699709658941
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.923984625702815	29.51178412617248
2	17.798551260070937	16.43411328694385
3	7.567706799672955	10.481348935450239
4	3.690795439513449	6.815717693604912
5	2.0629260558629383	4.761955061575146
6	1.2395839134480442	3.4336720159880882
7	0.8178767434574777	2.643123926839856
8	0.5359296980969924	1.9793811852815912
9	0.4137404345255346	1.7191038095533795
>10	1.849145508450541	14.567795238096013
>50	0.06710566170204332	2.0571263852317294
>100	0.03023327911983823	2.728160581520446
>500	0.0019853845353266395	0.6042633173322701
>1k	2.901337586667771E-4	0.21652975612564485
>5k	1.0879656182865019E-4	0.3510860863780246
>10k+	3.6265520609550064E-5	1.6948385939062858
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	164751	1.3304783070765671	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37706	0.3045020366894832	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.429636496958694E-4	0.0	0.0	0.0	8.07569184452032E-6
2	9.529316376533978E-4	0.0	0.0	0.0	8.07569184452032E-6
3	9.61007329497918E-4	0.0	0.0	0.0	2.422707553356096E-5
4	9.690830213424384E-4	0.0	0.0	0.0	2.422707553356096E-5
5	0.0015020786830807794	0.0	0.0	0.0	2.422707553356096E-5
6	0.0015182300667698201	0.0	0.0	0.0	3.230276737808128E-5
7	0.0015182300667698201	0.0	0.0	0.0	3.230276737808128E-5
8	0.0015182300667698201	0.0	0.0	0.0	3.230276737808128E-5
9	0.0015182300667698201	0.0	0.0	0.0	4.03784592226016E-5
10	0.0015343814504588608	0.0	0.0	0.0	4.845415106712192E-5
11	0.0015747599096814623	0.0	0.0	0.0	4.845415106712192E-5
12	0.0015828356015259828	0.0	0.0	8.07569184452032E-6	5.652984291164224E-5
13	0.00176857651394995	0.0	0.0	8.07569184452032E-6	6.460553475616256E-5
14	0.001817030665017072	0.0	0.0	8.07569184452032E-6	8.883261028972352E-5
15	0.001922014658995836	0.0	0.0	8.07569184452032E-6	8.883261028972352E-5
16	0.001962393118218438	0.0	0.0	8.07569184452032E-6	8.883261028972352E-5
17	0.001962393118218438	0.0	0.0	8.07569184452032E-6	8.883261028972352E-5
18	0.0020593014203526814	0.0	0.0	8.07569184452032E-6	9.690830213424384E-5
19	0.0020835284958862425	8.07569184452032E-6	0.0	8.07569184452032E-6	1.0498399397876416E-4
20	0.0021077555714198036	8.07569184452032E-6	0.0	1.615138368904064E-5	1.211353776678048E-4
21	0.0021642854143314456	8.07569184452032E-6	0.0	1.615138368904064E-5	1.2921106951232513E-4
22	0.002204663873554047	8.07569184452032E-6	0.0	1.615138368904064E-5	1.2921106951232513E-4
23	0.002245042332776649	8.07569184452032E-6	0.0	1.615138368904064E-5	1.615138368904064E-4
24	0.0022854207919992507	8.07569184452032E-6	0.0	1.615138368904064E-5	1.695895287349267E-4
25	0.002350026326755413	8.07569184452032E-6	0.0	1.615138368904064E-5	1.7766522057944704E-4
26	0.0023823290941334943	2.422707553356096E-5	0.0	1.615138368904064E-5	2.0996798795752832E-4
27	0.00251961585549034	2.422707553356096E-5	0.0	1.615138368904064E-5	2.0996798795752832E-4
28	0.0025599943147129414	2.422707553356096E-5	0.0	1.615138368904064E-5	2.0996798795752832E-4
29	0.0027861136863595102	2.422707553356096E-5	0.0	1.615138368904064E-5	2.341950634910893E-4
30	0.0029072490640273153	2.422707553356096E-5	0.0	1.615138368904064E-5	2.341950634910893E-4
31	0.0030606872090732013	2.422707553356096E-5	0.0	1.615138368904064E-5	2.341950634910893E-4
32	0.003189898278585526	2.422707553356096E-5	0.0	1.615138368904064E-5	2.422707553356096E-4
33	0.003383714882854014	2.422707553356096E-5	0.0	1.615138368904064E-5	2.503464471801299E-4
34	0.0035936828708115426	2.422707553356096E-5	0.0	1.615138368904064E-5	2.6649783086917054E-4
35	0.0038278779343026317	2.422707553356096E-5	0.0	1.615138368904064E-5	2.9072490640273153E-4
36	0.004368949287885493	3.230276737808128E-5	0.0	1.615138368904064E-5	3.0687629009177214E-4
37	0.004603144351376582	3.230276737808128E-5	0.0	1.615138368904064E-5	3.230276737808128E-4
38	0.004659674194288224	3.230276737808128E-5	0.0	1.615138368904064E-5	3.3110336562533314E-4
39	0.0047646581882669885	3.230276737808128E-5	0.0	1.615138368904064E-5	3.4725474931437375E-4
40	0.004813112339334111	3.230276737808128E-5	0.0	1.615138368904064E-5	3.4725474931437375E-4
41	0.004837339414867672	3.230276737808128E-5	0.0	1.615138368904064E-5	3.4725474931437375E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	52205	0.0	42.764103	12
CAACGCA	52300	0.0	42.645985	13
AACGCAG	52650	0.0	42.304462	14
ATCAACG	52855	0.0	42.22931	11
ACGCAGA	52625	0.0	42.19506	15
GTGGTAT	52445	0.0	42.156162	6
AAGCAGT	52645	0.0	42.058506	1
GGTATCA	52950	0.0	41.89613	8
GTATCAA	53160	0.0	41.735046	9
TGGTATC	52955	0.0	41.732414	7
AGTGGTA	52920	0.0	41.697845	5
CGCAGAG	53500	0.0	41.48738	16
TATCAAC	53665	0.0	41.33793	10
AGAGTAC	52220	0.0	41.16775	19
CAGAGTA	53835	0.0	40.12046	18
AGCAGTG	55570	0.0	39.87007	2
CAGTGGT	55305	0.0	39.814663	4
GCAGAGT	54560	0.0	39.755314	17
GCAGTGG	55775	0.0	39.71089	3
GAGTACT	27345	0.0	39.58347	20
>>END_MODULE