FastQCFastQC Report
Thu 26 May 2016
SRR805312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17205786
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2588991.5047205631872906No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG264630.15380291257836173No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC195290.11350251595596969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1152350.044.9810812
CAACGCA1154800.044.89175813
ACGCAGA1159900.044.58091415
AACGCAG1162350.044.56579214
GTATCAA1152950.044.5561379
ATCAACG1162000.044.54685611
GTGGTAT1151650.044.5370566
GGTATCA1155450.044.484148
AGTGGTA1154350.044.353495
TGGTATC1161100.044.1685037
CGCAGAG1171750.044.0999816
TATCAAC1167300.044.00235410
AGAGTAC1142650.043.83074619
AAGCAGT1180550.043.7871781
CAGAGTA1167350.043.0885318
CAGTGGT1193500.042.9340174
GCAGAGT1181650.042.74806217
AGCAGTG1213000.042.627412
GCAGTGG1214650.042.559833
GTACATG739350.042.46750322
Produced by FastQC (version 0.11.5)