FastQCFastQC Report
Thu 26 May 2016
SRR805311_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805311_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17749607
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2673871.5064389876350501No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA391370.22049502279120883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT836650.041.126751
AGTGGTA848800.040.086775
AGAGTAC832650.039.95268219
GGTATCA856950.039.867328
CAACGCA858150.039.85812813
TCAACGC858850.039.8475312
TGGTATC858000.039.741847
GTGGTAT858950.039.6513756
ACGCAGA863800.039.4641115
GTATCAA865500.039.4110349
AACGCAG867200.039.4042314
GAGTACT464500.039.16329220
ATCAACG882800.038.90756611
CGCAGAG878500.038.8492316
CAGTGGT883550.038.68574
TATCAAC889600.038.36448710
GTACTTT474400.038.30638522
GCAGTGG899700.038.249863
AGCAGTG907100.037.927462
CAGAGTA883350.037.7207818
Produced by FastQC (version 0.11.5)