##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805311_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17749607
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.94635593903572	35.0	35.0	37.0	32.0	37.0
2	35.31937078944903	36.0	35.0	37.0	33.0	37.0
3	37.05108952553147	39.0	37.0	39.0	33.0	39.0
4	37.14468697813985	39.0	37.0	39.0	33.0	39.0
5	37.15234213354696	39.0	37.0	39.0	33.0	39.0
6	37.04822816640391	39.0	37.0	39.0	33.0	39.0
7	36.96657999244716	39.0	37.0	39.0	33.0	39.0
8	37.83388060366632	40.0	37.0	40.0	33.0	40.0
9	37.79884168703003	40.0	37.0	40.0	33.0	40.0
10	37.631265300690885	40.0	37.0	40.0	33.0	40.0
11	37.68672641597079	40.0	37.0	40.0	33.0	40.0
12	37.58190375708037	40.0	37.0	40.0	33.0	40.0
13	37.689915895039256	40.0	37.0	40.0	33.0	40.0
14	37.595583045866874	40.0	37.0	40.0	32.0	40.0
15	37.52255523178626	40.0	37.0	40.0	32.0	40.0
16	37.43502737835266	40.0	37.0	40.0	32.0	40.0
17	37.30536952170265	39.0	37.0	40.0	32.0	40.0
18	37.14249701415924	39.0	37.0	40.0	32.0	40.0
19	37.13999898701982	39.0	36.0	40.0	32.0	40.0
20	36.997179543186505	39.0	36.0	40.0	31.0	40.0
21	36.88185648279424	39.0	36.0	40.0	31.0	40.0
22	36.79584393051632	39.0	36.0	40.0	31.0	40.0
23	36.59110210158456	39.0	36.0	40.0	30.0	40.0
24	36.634160970437264	39.0	36.0	40.0	30.0	40.0
25	36.5822717088891	39.0	36.0	40.0	30.0	40.0
26	36.489927467126456	39.0	36.0	40.0	30.0	40.0
27	36.39678574291814	39.0	36.0	40.0	30.0	40.0
28	36.27978439184597	39.0	35.0	40.0	30.0	40.0
29	36.13962303503396	38.0	35.0	40.0	30.0	40.0
30	36.069546046850505	38.0	35.0	40.0	30.0	40.0
31	35.92141949959793	38.0	35.0	40.0	29.0	40.0
32	35.79071446483294	38.0	35.0	40.0	28.0	40.0
33	35.64893498768733	38.0	34.0	40.0	27.0	40.0
34	35.55285488856176	38.0	34.0	40.0	27.0	40.0
35	35.453008565203724	38.0	34.0	40.0	27.0	40.0
36	35.32974701918752	38.0	34.0	40.0	27.0	40.0
37	35.2469836092709	38.0	34.0	40.0	27.0	40.0
38	35.4212142274474	38.0	34.0	40.0	27.0	40.0
39	35.42913299432489	38.0	34.0	40.0	27.0	40.0
40	35.29174234674604	38.0	34.0	40.0	27.0	40.0
41	35.49990667399002	38.0	34.0	40.0	27.0	40.0
42	35.44326592695827	38.0	34.0	40.0	27.0	40.0
43	35.5147489181028	38.0	34.0	40.0	27.0	40.0
44	35.507256470523544	38.0	34.0	40.0	27.0	40.0
45	35.42562514201018	38.0	34.0	40.0	27.0	40.0
46	35.26201599843873	38.0	34.0	40.0	26.0	40.0
47	35.21025389463553	38.0	34.0	40.0	26.0	40.0
48	35.159402909596814	38.0	34.0	40.0	26.0	40.0
49	35.042987656008385	38.0	34.0	40.0	26.0	40.0
50	34.93412141463188	38.0	33.0	40.0	26.0	40.0
51	34.77684784795517	38.0	33.0	40.0	26.0	40.0
52	34.52566087801268	37.0	33.0	40.0	25.0	40.0
53	33.92375864998025	37.0	32.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	7.0
12	25.0
13	103.0
14	279.0
15	726.0
16	1802.0
17	3980.0
18	8163.0
19	15021.0
20	25539.0
21	39519.0
22	57289.0
23	79449.0
24	106059.0
25	137119.0
26	173891.0
27	216920.0
28	267738.0
29	328563.0
30	399867.0
31	486771.0
32	595848.0
33	737575.0
34	936803.0
35	1234714.0
36	1701355.0
37	2481385.0
38	3728393.0
39	3984700.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.120714729064144	39.90002708228976	14.172099697756687	22.807158490889403
2	26.74547104057008	30.304242792530562	27.917823758013345	15.03246240888601
3	24.362702791109687	25.508925352544427	26.567512170832853	23.560859685513037
4	20.346985710725875	29.70128859754472	24.76689765581852	25.18482803591088
5	21.71770338351717	32.196538210677	23.90877724785681	22.17698115794902
6	25.22438947521486	25.46483986941232	28.079985094881255	21.230785560491565
7	21.05924936816911	26.953740440562996	29.132515441046102	22.854494750221793
8	23.774526388105382	25.316481655058613	27.937886174043175	22.971105782792826
9	25.213707548567132	25.593710328346987	27.373034231124105	21.819547891961776
10	21.343897924049813	26.94974598592521	30.51194879976779	21.194407290257185
11	21.294409504390714	30.912284424100207	26.75778117228173	21.035524899227344
12	21.314319804376513	26.08083660669219	30.65565902388712	21.94918456504417
13	21.67263196306262	26.58174910576893	25.986350007636787	25.759268923531657
14	21.728728979745863	30.400859016202443	26.026587518247585	21.84382448580411
15	21.767608713815466	30.083353394810374	25.909514503616897	22.239523387757263
16	22.386377343453294	26.183762829227714	25.424100939248966	26.00575888807003
17	26.332211186422327	26.0681151982689	25.627474456195003	21.972199159113774
18	22.319457551933404	25.87635883994502	25.559061673872552	26.24512193424902
19	22.541563878005864	30.23277642147232	25.159464094050083	22.066195606471737
20	26.43263031119506	26.19956036209703	25.529894830910905	21.837914495797005
21	22.51893239101012	29.779008628191033	25.57471835855295	22.12734062224589
22	26.46894660822631	26.10423430783566	25.472603421585617	21.95421566235241
23	22.23380495128709	26.041630104824293	29.93255005589701	21.792014887991602
24	22.17338107824021	29.839342358397005	25.573805662288745	22.413470901074035
25	22.24828977903567	26.080492937111227	25.479104973986182	26.192112309866918
26	22.1951449403922	27.730287211429527	28.199086323432397	21.875481524745872
27	22.288780816386527	25.550362889724827	29.915625737516326	22.24523055637232
28	24.31197490738809	26.013325252778834	27.583005077239175	22.0916947625939
29	24.35327159637957	25.879936383943598	27.862870428624138	21.90392159105269
30	24.254018694611098	25.609682512970565	27.83727549573351	22.299023296684823
31	23.801450927899417	26.22189325093226	27.587709406749116	22.388946414419202
32	23.26881941667779	26.407407217523183	28.025527551117047	22.29824581468198
33	23.23488627100307	26.01037870866662	27.93076488961136	22.82397013071895
34	23.1656283995471	26.3942801663158	27.75862023311277	22.68147120102434
35	22.975443907011574	26.335484498332836	28.169096927047455	22.519974667608132
36	23.105739749618117	26.107659735790207	28.033764353205115	22.752836161386558
37	23.31312462298461	26.42772316029307	27.64800933338975	22.611142883332572
38	23.129171254326927	26.362037198908123	28.155795224085807	22.352996322679143
39	23.125988085257323	26.118612090960664	28.07596247060569	22.679437353176326
40	23.276498459937734	26.519601250889668	27.721977168283217	22.48192312088938
41	22.934079610889412	26.53581569439819	28.1248874975091	22.405217197203296
42	23.14736884033545	26.137226587608392	28.182302853240635	22.53310171881552
43	23.056887963772947	26.522254830768926	27.843129146465046	22.57772805899308
44	23.03545086941925	26.34385088075471	28.162831999604272	22.457866250221766
45	23.068730479497376	26.01268298503736	28.144685118943762	22.773901416521504
46	23.065107864078342	26.33248724887261	27.80937065254459	22.793034234504457
47	22.86344142718202	26.296170951841358	28.127050925690916	22.713336695285705
48	22.925594915988846	26.079946446138212	28.049364698609946	22.945093939262993
49	22.980277816855324	26.648944959739108	27.64954739561276	22.721229827792808
50	23.029185941976067	26.518570242146772	27.96513184770795	22.48711196816921
51	22.990869600662144	26.251550245591353	27.94941318982443	22.808166963922076
52	23.210125159390856	26.565692412232	27.45000494940536	22.774177478971787
53	23.268064470385173	26.5143786000445	27.49459185209002	22.722965077480307
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	44888.0
1	26993.5
2	9099.0
3	7237.5
4	5376.0
5	5242.5
6	5109.0
7	5378.0
8	6196.0
9	6745.0
10	8197.5
11	9650.0
12	12503.0
13	15356.0
14	18617.5
15	21879.0
16	28587.5
17	35296.0
18	47205.0
19	59114.0
20	76688.0
21	94262.0
22	266132.5
23	438003.0
24	336947.0
25	259156.5
26	282422.0
27	319438.5
28	356455.0
29	389219.5
30	421984.0
31	445418.5
32	468853.0
33	510297.5
34	551742.0
35	614203.0
36	676664.0
37	733952.0
38	791240.0
39	840785.0
40	890330.0
41	938079.5
42	1024277.0
43	1062725.0
44	1091134.5
45	1119544.0
46	1145914.5
47	1172285.0
48	1181793.0
49	1191301.0
50	1184155.5
51	1177010.0
52	1145920.5
53	1114831.0
54	1070772.0
55	1026713.0
56	973585.0
57	920457.0
58	838103.0
59	669479.0
60	583209.0
61	510046.0
62	436883.0
63	372698.0
64	308513.0
65	255042.0
66	201571.0
67	162227.5
68	122884.0
69	95810.5
70	68737.0
71	52347.5
72	35958.0
73	27299.0
74	18640.0
75	13901.5
76	7031.5
77	4900.0
78	3920.5
79	2941.0
80	2479.5
81	2018.0
82	1524.0
83	1030.0
84	707.5
85	385.0
86	274.5
87	164.0
88	128.5
89	93.0
90	64.0
91	35.0
92	26.5
93	14.5
94	11.0
95	6.5
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.7749607E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.50307263392421
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.569046085308706	20.438780246324455
2	17.332282908012168	12.306985979897583
3	9.026533818845266	9.614090574091103
4	5.197658483240006	7.381313866272092
5	3.063849784270055	5.43880407151864
6	1.916048841518142	4.081537271433897
7	1.2398402372358726	3.0812696597932914
8	0.881833218997757	2.504623106006765
9	0.6162787400835698	1.9691809984737252
>10	2.8703791041423616	17.628179412162577
>50	0.1556968716915341	3.8328842649123738
>100	0.12697844691975133	8.640248549612807
>500	0.0029067463871055113	0.6641104186424712
>1k	5.239060038785503E-4	0.302712269149367
>5k	6.346993057228054E-5	0.1885851675928303
>10k+	7.933741321535067E-5	1.926694144115901
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	267387	1.5064389876350501	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39137	0.22049502279120883	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.056427840909379E-4	2.816963778409291E-5	0.0	0.0	0.0
2	4.056427840909379E-4	4.5071420454548655E-5	0.0	0.0	0.0
3	4.056427840909379E-4	5.070534801136724E-5	0.0	0.0	0.0
4	4.056427840909379E-4	5.070534801136724E-5	0.0	0.0	0.0
5	4.056427840909379E-4	5.633927556818582E-5	0.0	0.0	1.1267855113637164E-5
6	4.1691063920457506E-4	6.760713068182298E-5	0.0	0.0	2.2535710227274328E-5
7	4.1691063920457506E-4	7.324105823864157E-5	0.0	0.0	2.2535710227274328E-5
8	4.1691063920457506E-4	7.324105823864157E-5	0.0	5.633927556818582E-6	3.943749289773007E-5
9	4.1691063920457506E-4	9.577676846591589E-5	0.0	1.1267855113637164E-5	4.5071420454548655E-5
10	4.5634813210230514E-4	1.1831247869319022E-4	0.0	1.1267855113637164E-5	5.070534801136724E-5
11	4.901516974432167E-4	1.239464062500088E-4	0.0	1.1267855113637164E-5	5.633927556818582E-5
12	5.239552627841281E-4	1.577499715909203E-4	0.0	1.1267855113637164E-5	6.19732031250044E-5
13	6.253659588068626E-4	1.7465175426137605E-4	0.0	1.1267855113637164E-5	6.760713068182298E-5
14	7.211427272727785E-4	1.7465175426137605E-4	0.0	1.1267855113637164E-5	7.887498579546015E-5
15	8.450891335227873E-4	1.7465175426137605E-4	0.0	1.1267855113637164E-5	7.887498579546015E-5
16	9.352319744318846E-4	1.7465175426137605E-4	0.0	1.1267855113637164E-5	1.1831247869319022E-4
17	0.0010929819460228048	1.7465175426137605E-4	0.0	1.1267855113637164E-5	1.1831247869319022E-4
18	0.0012619997727273623	1.7465175426137605E-4	0.0	1.1267855113637164E-5	1.2958033380682738E-4
19	0.0013690443963069155	2.0282139204546895E-4	0.0	1.1267855113637164E-5	1.6901782670455747E-4
20	0.0015267943678978356	2.0282139204546895E-4	0.0	1.1267855113637164E-5	1.6901782670455747E-4
21	0.0016620086292614817	2.0845531960228753E-4	0.0	1.1267855113637164E-5	1.6901782670455747E-4
22	0.0017915889630683091	2.197231747159247E-4	0.0	1.1267855113637164E-5	1.8028568181819462E-4
23	0.0019324371519887735	2.42258884943199E-4	0.0	1.1267855113637164E-5	1.8028568181819462E-4
24	0.002197231747159247	2.760624502841105E-4	0.0	1.1267855113637164E-5	1.8028568181819462E-4
25	0.002484562052556995	2.9859816051138485E-4	0.0	1.1267855113637164E-5	2.0845531960228753E-4
26	0.0027042852272729195	3.09866015625022E-4	0.0	1.1267855113637164E-5	2.2535710227274328E-4
27	0.0029071066193183883	3.2676779829547773E-4	0.0	1.1267855113637164E-5	2.42258884943199E-4
28	0.0032282404900570473	3.2676779829547773E-4	0.0	2.816963778409291E-5	2.535267400568362E-4
29	0.003532472578125251	3.3803565340911494E-4	0.0	4.5071420454548655E-5	2.535267400568362E-4
30	0.0038592403764207287	3.493035085227521E-4	0.0	5.070534801136724E-5	2.760624502841105E-4
31	0.004247981377841211	3.662052911932078E-4	0.0	5.070534801136724E-5	2.760624502841105E-4
32	0.004709963437500335	3.718392187500264E-4	0.0	5.070534801136724E-5	2.8169637784092907E-4
33	0.005459275802557206	3.8310707386366355E-4	0.0	6.19732031250044E-5	2.873303053977477E-4
34	0.006225489950284533	3.8310707386366355E-4	0.0	6.19732031250044E-5	2.873303053977477E-4
35	0.007064945156250502	3.8310707386366355E-4	0.0	6.19732031250044E-5	3.0423208806820343E-4
36	0.007791721811080099	3.9437492897730075E-4	0.0	6.19732031250044E-5	3.3803565340911494E-4
37	0.008248069943182404	4.056427840909379E-4	0.0	6.19732031250044E-5	3.3803565340911494E-4
38	0.008597373451705156	4.2254456676139363E-4	0.0	6.19732031250044E-5	3.3803565340911494E-4
39	0.008963578742898364	4.394463494318494E-4	0.0	6.19732031250044E-5	3.493035085227521E-4
40	0.009256542975852931	4.7324991477276087E-4	0.0	6.19732031250044E-5	3.662052911932078E-4
41	0.009634016122159776	4.7324991477276087E-4	0.0	6.19732031250044E-5	4.056427840909379E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	83665	0.0	41.12675	1
AGTGGTA	84880	0.0	40.08677	5
AGAGTAC	83265	0.0	39.952682	19
GGTATCA	85695	0.0	39.86732	8
CAACGCA	85815	0.0	39.858128	13
TCAACGC	85885	0.0	39.84753	12
TGGTATC	85800	0.0	39.74184	7
GTGGTAT	85895	0.0	39.651375	6
ACGCAGA	86380	0.0	39.46411	15
GTATCAA	86550	0.0	39.411034	9
AACGCAG	86720	0.0	39.40423	14
GAGTACT	46450	0.0	39.163292	20
ATCAACG	88280	0.0	38.907566	11
CGCAGAG	87850	0.0	38.84923	16
CAGTGGT	88355	0.0	38.6857	4
TATCAAC	88960	0.0	38.364487	10
GTACTTT	47440	0.0	38.306385	22
GCAGTGG	89970	0.0	38.24986	3
AGCAGTG	90710	0.0	37.92746	2
CAGAGTA	88335	0.0	37.72078	18
>>END_MODULE