FastQCFastQC Report
Thu 26 May 2016
SRR805310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12824122
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA872340.6802337033287736No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA761490.5937950371963087No Hit
ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG688230.5366683192814292No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCG642190.5007672260136016No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT634840.4950358394906099No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT629800.4911057458748443No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT622270.48523399886557533No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT596430.46508447127998315No Hit
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG593050.46244881325988635No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA579440.45183600093636045No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC579270.45170343825487624No Hit
CTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC565440.4409190742259002No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC563670.4395388627775063No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC561480.43783114352779867No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG560610.43715273451079145No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC557670.43486017990159487No Hit
AGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG551950.43039983555989253No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC523980.4085893755533517No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA514740.401384203924448No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACT507770.39594913398359743No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA465390.36290203727007586No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA444890.3469165374440449No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA430290.33553174244599354No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT410780.320318225294488No Hit
TACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTG395490.30839538176570686No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA391420.3052216752148802No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA374190.291786057556221No Hit
TCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT370330.28877610490605127No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC356960.2783504398975618No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG356580.27805412331542073No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT340290.265351499307321No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA337360.26306674250291756No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT326190.25435659454892895No Hit
TGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG322450.2514402155562775No Hit
TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT313320.24432081978009879No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC277310.21624092471983655No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA271380.21161682647747737No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG245360.19132693840560783No Hit
TGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC236070.18408277775273815No Hit
GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT235470.183614909465147No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT225960.17619919710682727No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG176780.1378495931339393No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC166820.1300829795599262No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA163320.12735374788231119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC84200.022.80225819
GAGTACT40900.021.54645520
GTACTTT46550.018.72932222
AGTACTT51300.017.45321721
ACATGGG59900.017.30133424
GTACATG60250.017.2008322
GAGTACA62850.016.78838520
TACATGG64850.016.01696223
AGTACAT66800.015.65494121
TACTTTT61000.014.63934423
CATGGGG47350.014.39281825
ACTTTTT69100.013.05933424
CTTTTTT95250.09.57270325
ATGGGGG33200.08.84789226
ATGGGGC24200.06.797520626
CATGGGA33400.06.47305425
GCTTGAA28000.06.210714329
ATGGGGA34400.06.079941726
GCTTACG11903.783498E-95.52941236
TGGGGGG33300.05.433933727
Produced by FastQC (version 0.11.5)