FastQCFastQC Report
Thu 26 May 2016
SRR805309_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805309_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22048481
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1907130.8649711515274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1080550.042.7438812
CAACGCA1083950.042.62715513
AAGCAGT1075950.042.555331
AGTGGTA1074950.042.4877935
GTGGTAT1077850.042.475956
GGTATCA1082650.042.389658
GTATCAA1083800.042.3164839
AACGCAG1092400.042.26515214
TGGTATC1083850.042.229977
ATCAACG1093350.042.22842811
ACGCAGA1094500.042.16903315
TATCAAC1101200.041.65424310
CGCAGAG1114000.041.38869516
AGAGTAC1091250.041.03482419
CAGTGGT1125400.040.6833574
AGCAGTG1133750.040.43142
GCAGTGG1142850.040.3438843
CAGAGTA1120200.040.1316718
GCAGAGT1138750.039.68430317
GAGTACA773950.039.33923320
Produced by FastQC (version 0.11.5)