##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805309_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22048481
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.665387697229576	37.0	35.0	37.0	35.0	37.0
2	35.6076683015034	37.0	35.0	37.0	33.0	37.0
3	37.28047986616402	39.0	37.0	39.0	34.0	39.0
4	37.27283752563272	39.0	37.0	39.0	34.0	39.0
5	37.274577055897865	39.0	37.0	39.0	34.0	39.0
6	37.22372062728493	39.0	37.0	39.0	34.0	39.0
7	37.07437968175676	39.0	37.0	39.0	33.0	39.0
8	37.987844060550024	40.0	38.0	40.0	33.0	40.0
9	37.92023631922761	40.0	38.0	40.0	33.0	40.0
10	37.7172865105764	40.0	37.0	40.0	33.0	40.0
11	37.8045435873791	40.0	37.0	40.0	33.0	40.0
12	37.71527453523896	40.0	37.0	40.0	33.0	40.0
13	37.814956232132275	40.0	37.0	40.0	33.0	40.0
14	37.69984979917664	40.0	37.0	40.0	33.0	40.0
15	37.669569980807296	40.0	37.0	40.0	33.0	40.0
16	37.54591007879409	40.0	37.0	40.0	32.0	40.0
17	37.41701144854378	40.0	37.0	40.0	32.0	40.0
18	37.3934758135946	39.0	37.0	40.0	32.0	40.0
19	37.363454924627234	39.0	37.0	40.0	32.0	40.0
20	37.17548614800267	39.0	37.0	40.0	32.0	40.0
21	37.078780846626124	39.0	36.0	40.0	32.0	40.0
22	36.950394950110166	39.0	36.0	40.0	31.0	40.0
23	36.73087157342041	39.0	36.0	40.0	30.0	40.0
24	36.77828926174098	39.0	36.0	40.0	31.0	40.0
25	36.745346266711074	39.0	36.0	40.0	30.0	40.0
26	36.644275675952464	39.0	36.0	40.0	30.0	40.0
27	36.502094316610744	39.0	36.0	40.0	30.0	40.0
28	36.400420691112465	39.0	36.0	40.0	30.0	40.0
29	36.290123977248136	38.0	35.0	40.0	30.0	40.0
30	36.18295686673381	38.0	35.0	40.0	30.0	40.0
31	36.03905883584452	38.0	35.0	40.0	29.0	40.0
32	35.94929092847711	38.0	35.0	40.0	29.0	40.0
33	35.843212419032405	38.0	35.0	40.0	29.0	40.0
34	35.86258862912143	38.0	35.0	40.0	29.0	40.0
35	35.884231888809026	38.0	35.0	40.0	29.0	40.0
36	35.86167128701519	38.0	35.0	40.0	29.0	40.0
37	35.830788161778585	38.0	35.0	40.0	29.0	40.0
38	35.774735955733185	38.0	35.0	40.0	29.0	40.0
39	35.72086190427359	38.0	34.0	40.0	28.0	40.0
40	35.67089651209986	38.0	34.0	40.0	28.0	40.0
41	35.619791177451184	38.0	34.0	40.0	28.0	40.0
42	35.377027288183704	38.0	34.0	40.0	27.0	40.0
43	35.39392704649359	38.0	34.0	40.0	27.0	40.0
44	35.28318005217684	38.0	34.0	40.0	27.0	40.0
45	35.1456249072215	38.0	34.0	40.0	27.0	40.0
46	34.996109255780475	38.0	33.0	40.0	26.0	40.0
47	35.177850528569294	38.0	34.0	40.0	27.0	40.0
48	35.2428071121997	38.0	34.0	40.0	27.0	40.0
49	35.14780473992744	38.0	34.0	40.0	26.0	40.0
50	35.04303670624747	38.0	34.0	40.0	26.0	40.0
51	34.87811128576159	38.0	33.0	40.0	26.0	40.0
52	34.66113661072615	37.0	33.0	40.0	26.0	40.0
53	34.17162338757033	37.0	32.0	40.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	11.0
12	44.0
13	126.0
14	291.0
15	707.0
16	1676.0
17	3788.0
18	7941.0
19	14701.0
20	25100.0
21	38889.0
22	57099.0
23	80192.0
24	108725.0
25	144235.0
26	187416.0
27	238361.0
28	301709.0
29	376977.0
30	466873.0
31	580464.0
32	718982.0
33	903934.0
34	1162393.0
35	1542294.0
36	2136201.0
37	3143827.0
38	4752529.0
39	5052992.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	18.845475114589526	32.181414220780105	26.294391890307544	22.67871877432282
2	26.561117747748696	31.769807634367194	22.43926010141016	19.229814516473947
3	28.218497228902073	25.25510487547872	23.22735067327314	23.299047222346065
4	22.619948285779866	26.445844500580336	23.121320693248666	27.81288652039113
5	23.415726462063304	30.259590218482625	23.882502381910122	22.442180937543952
6	27.621930054954806	24.701819594737614	24.545382514106073	23.130867836201503
7	23.289232487262954	24.934162131169035	28.979941974233963	22.796663407334048
8	26.699517304616133	25.15589622704621	25.216385654866656	22.928200813471005
9	26.95583428173578	24.88944249719516	25.331450270882605	22.823272950186453
10	22.50658446720207	25.432817798196623	29.523507764548494	22.53708997005281
11	22.374130898178425	29.832876015359062	25.235362018816627	22.557631067645882
12	22.313333059089196	25.120646633207976	29.67609877523989	22.88992153246294
13	22.4811949630453	25.320982429583243	25.114677968065013	27.083144639306443
14	22.650911870073955	29.730850846368963	24.98161211196363	22.63662517159345
15	22.56219827569981	29.582387104127488	24.937377772192107	22.918036847980595
16	22.793484049989658	25.09818703610466	24.906636425429944	27.201692488475736
17	27.313165020302304	25.00914235316256	24.904577326664818	22.773115299870316
18	22.793484049989658	24.933694978806024	24.85680532822193	27.416015642982387
19	22.905242315785838	29.47774043935272	24.848646035978625	22.768371208882826
20	27.418972762794862	25.07423527271561	24.86681508807795	22.639976876411577
21	22.998210171485283	29.284670449633243	24.853730286453747	22.863389092427727
22	27.322834620670694	24.9474102093473	24.984215465908967	22.745539704073035
23	22.975165500063248	24.957451717422167	29.39331738998256	22.674065392532032
24	22.96186299636696	29.178209600924436	24.866107556343678	22.99381984636493
25	22.858926199950012	25.01221739493074	24.875999394243983	27.252857010875264
26	23.03505171172563	27.927969278246422	26.29978908751129	22.737189922516656
27	23.074315187517907	24.79081438762153	29.26872830831294	22.86614211654762
28	26.08678121635681	24.891474383201274	26.206848444570852	22.814895955871066
29	26.200299240569	24.905117046385193	26.148522431091735	22.746061281954074
30	26.131482708491347	24.7605039095437	26.121191750125554	22.986821631839398
31	25.10874558660073	25.340562009691283	26.328131176020698	23.222561227687294
32	24.444795085883694	25.803886444603595	26.45145486439633	23.299863605116382
33	24.274488569076482	25.461432014296133	26.60863122498099	23.6554481916464
34	23.934678311852867	25.476435315430574	26.82958975722636	23.7592966154902
35	23.889786330405254	25.58457428427836	26.903803486507755	23.62183589880863
36	23.936864403493374	25.502378145687228	26.865433496302987	23.69532395451641
37	24.123725348698624	25.521599424468288	26.794154209534888	23.560521017298196
38	24.15154132386716	25.556563284336914	26.863950400936915	23.427944990859007
39	24.182500372701412	25.442414831207643	26.772778587332162	23.60230620875878
40	23.96359640376133	25.703367048278746	26.6555142732962	23.67752227466373
41	24.060637102392676	25.82331635453708	26.63810264298933	23.477943900080916
42	24.242772098449773	25.62854556737945	26.681615844647077	23.4470664895237
43	24.02686606846068	25.657268634515003	26.826977332361352	23.488887964662965
44	24.133272491651468	25.52305530707535	26.72622209212508	23.61745010914811
45	24.155899900768674	25.290191192762894	26.703676321284895	23.850232585183534
46	24.057103979181154	25.480861924229607	26.566623795988487	23.89541030060075
47	23.99665537049922	25.50152547923823	26.720539160951724	23.78127998931083
48	23.967868806925974	25.387894068530166	26.688482530837387	23.955754593706477
49	23.8625372877161	25.78727759068754	26.53644484624587	23.813740275350487
50	24.124940851934426	25.68321600023149	26.57706895998867	23.614774187845413
51	24.0485773146912	25.47252574905274	26.647731424219202	23.83116551203686
52	24.178377639711325	25.583549270355633	26.458203628630926	23.77986946130212
53	24.346879043504178	25.62578800779972	26.243163871470333	23.784169077225773
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4545.0
1	3010.5
2	1476.0
3	1390.5
4	1305.0
5	1412.0
6	1519.0
7	1783.0
8	2480.0
9	2913.0
10	3897.5
11	4882.0
12	7102.0
13	9322.0
14	11512.0
15	13702.0
16	19371.5
17	25041.0
18	34791.0
19	44541.0
20	60448.5
21	76356.0
22	203683.5
23	331011.0
24	271731.5
25	232408.5
26	252365.0
27	285154.5
28	317944.0
29	351029.0
30	384114.0
31	417697.0
32	451280.0
33	502640.0
34	554000.0
35	619997.5
36	685995.0
37	759203.0
38	832411.0
39	903642.0
40	974873.0
41	1048248.0
42	1194018.5
43	1266414.0
44	1336099.0
45	1405784.0
46	1462133.0
47	1518482.0
48	1561734.5
49	1604987.0
50	1625504.0
51	1646021.0
52	1633478.0
53	1620935.0
54	1573678.0
55	1526421.0
56	1448445.5
57	1370470.0
58	1249543.5
59	998442.5
60	868268.0
61	760495.5
62	652723.0
63	557724.0
64	462725.0
65	379101.0
66	295477.0
67	237559.5
68	179642.0
69	139816.0
70	99990.0
71	74566.0
72	49142.0
73	36458.5
74	23775.0
75	17919.0
76	8922.5
77	5782.0
78	4184.0
79	2586.0
80	1998.0
81	1410.0
82	1058.5
83	707.0
84	455.0
85	203.0
86	144.5
87	86.0
88	54.0
89	22.0
90	18.5
91	15.0
92	12.0
93	5.5
94	2.0
95	2.0
96	2.0
97	2.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.2048481E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.121237300919724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.82557470125504	42.12016396487078
2	11.889173486137903	12.869135591101447
3	3.9600292670430295	6.42965051040669
4	1.8875785316572458	4.086323425437736
5	1.0132420493822114	2.741895669894244
6	0.6500176736284327	2.1107856458541714
7	0.4541900187140756	1.720692804777356
8	0.3248246721722697	1.4063930531063091
9	0.2594530929783004	1.2637730172182564
>10	1.5983559644348542	16.383855213140336
>50	0.10328791806147777	3.742209951288126
>100	0.032059218239298866	2.999831106289821
>500	0.0019211313118312633	0.6686780816117329
>1k	2.3392762041262108E-4	0.234283054294795
>5k	1.6670675321247136E-5	0.06326489876094855
>10k+	4.1676688303112875E-5	1.1590640119472033
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	190713	0.8649711515274	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0013606379505236665	0.0	0.0	4.5354598350788885E-6	2.7212759010473328E-5
2	0.006272540951914102	0.0	0.0	4.5354598350788885E-6	2.7212759010473328E-5
3	0.006304289170759655	0.0	0.0	4.5354598350788885E-6	3.1748218845552216E-5
4	0.00709799464189846	0.0	0.0	4.5354598350788885E-6	3.1748218845552216E-5
5	0.007111601021403697	1.3606379505236664E-5	0.0	9.070919670157777E-6	4.5354598350788885E-5
6	0.007143349240249249	1.3606379505236664E-5	0.0	9.070919670157777E-6	4.5354598350788885E-5
7	0.007143349240249249	1.3606379505236664E-5	0.0	9.070919670157777E-6	6.803189752618332E-5
8	0.007143349240249249	1.3606379505236664E-5	0.0	9.070919670157777E-6	6.803189752618332E-5
9	0.0071478847000843275	1.8141839340315554E-5	0.0	9.070919670157777E-6	9.070919670157777E-5
10	0.0071705619992597225	1.8141839340315554E-5	0.0	9.070919670157777E-6	1.0431557620681443E-4
11	0.0071705619992597225	2.2677299175394443E-5	0.0	9.070919670157777E-6	1.3152833521728777E-4
12	0.007274877575466536	2.2677299175394443E-5	0.0	9.070919670157777E-6	1.4513471472252443E-4
13	0.007288483954971774	2.2677299175394443E-5	0.0	9.070919670157777E-6	1.7688293356807664E-4
14	0.007297554874641931	3.1748218845552216E-5	0.0	9.070919670157777E-6	1.950247729083922E-4
15	0.007320232173817325	3.1748218845552216E-5	0.0	9.070919670157777E-6	2.0863115241362887E-4
16	0.007324767633652404	3.628367868063111E-5	0.0	9.070919670157777E-6	2.1770207208378662E-4
17	0.007338374013157641	3.628367868063111E-5	0.0	9.070919670157777E-6	2.267729917539444E-4
18	0.007338374013157641	4.0819138515709993E-5	0.0	9.070919670157777E-6	2.267729917539444E-4
19	0.007347444932827799	4.0819138515709993E-5	0.0	9.070919670157777E-6	2.4491483109426E-4
20	0.007356515852497957	4.5354598350788885E-5	0.0	1.3606379505236664E-5	2.585212105994966E-4
21	0.007370122232003193	4.5354598350788885E-5	0.0	1.3606379505236664E-5	2.6305667043457554E-4
22	0.00738372861150843	4.5354598350788885E-5	0.0	1.3606379505236664E-5	2.9480488928012774E-4
23	0.0073927995311785875	4.5354598350788885E-5	0.0	1.3606379505236664E-5	3.2201764829060107E-4
24	0.007433618669694298	4.989005818586777E-5	0.0	1.3606379505236664E-5	3.5376586713615327E-4
25	0.007451760509034613	4.989005818586777E-5	0.0	1.3606379505236664E-5	3.5376586713615327E-4
26	0.007492579647550323	5.896097785602555E-5	0.0	1.3606379505236664E-5	3.764431663115477E-4
27	0.007542469705736191	5.896097785602555E-5	0.0	1.3606379505236664E-5	3.809786261466266E-4
28	0.00758782430408698	5.896097785602555E-5	0.0	1.3606379505236664E-5	3.945850056518633E-4
29	0.007619572522932532	6.349643769110443E-5	0.0	1.8141839340315554E-5	4.1726230482725773E-4
30	0.007687604420458715	6.803189752618332E-5	0.0	1.8141839340315554E-5	4.3086868433249437E-4
31	0.007719352639304268	6.803189752618332E-5	0.0	3.1748218845552216E-5	4.3086868433249437E-4
32	0.007782849076995372	6.803189752618332E-5	0.0	3.1748218845552216E-5	4.3540414416757325E-4
33	0.007869022813861872	7.256735736126222E-5	0.0	3.1748218845552216E-5	4.535459835078888E-4
34	0.007927983791717897	7.256735736126222E-5	0.0	3.1748218845552216E-5	4.535459835078888E-4
35	0.008018692988419474	7.71028171963411E-5	0.0	3.1748218845552216E-5	4.580814433429677E-4
36	0.00808218942611058	9.070919670157777E-5	0.0	3.628367868063111E-5	4.716878228482044E-4
37	0.00819557592198755	9.070919670157777E-5	0.0	4.0819138515709993E-5	4.8982966218852E-4
38	0.008313497877699602	1.0431557620681443E-4	0.0	4.0819138515709993E-5	5.034360416937566E-4
39	0.008467703512092284	1.0431557620681443E-4	4.5354598350788885E-6	4.0819138515709993E-5	5.034360416937566E-4
40	0.008594696387474492	1.0431557620681443E-4	4.5354598350788885E-6	4.5354598350788885E-5	5.125069613639144E-4
41	0.008721689262856701	1.0431557620681443E-4	4.5354598350788885E-6	4.5354598350788885E-5	5.215778810340722E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	108055	0.0	42.74388	12
CAACGCA	108395	0.0	42.627155	13
AAGCAGT	107595	0.0	42.55533	1
AGTGGTA	107495	0.0	42.487793	5
GTGGTAT	107785	0.0	42.47595	6
GGTATCA	108265	0.0	42.38965	8
GTATCAA	108380	0.0	42.316483	9
AACGCAG	109240	0.0	42.265152	14
TGGTATC	108385	0.0	42.22997	7
ATCAACG	109335	0.0	42.228428	11
ACGCAGA	109450	0.0	42.169033	15
TATCAAC	110120	0.0	41.654243	10
CGCAGAG	111400	0.0	41.388695	16
AGAGTAC	109125	0.0	41.034824	19
CAGTGGT	112540	0.0	40.683357	4
AGCAGTG	113375	0.0	40.4314	2
GCAGTGG	114285	0.0	40.343884	3
CAGAGTA	112020	0.0	40.13167	18
GCAGAGT	113875	0.0	39.684303	17
GAGTACA	77395	0.0	39.339233	20
>>END_MODULE