FastQCFastQC Report
Thu 26 May 2016
SRR805308_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805308_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19056391
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1369120.7184571307337261No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA464140.24356133330807497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC407300.032.58716219
GAGTACT237150.031.16698320
GTACTTT250300.029.26887722
AGTACTT254200.029.1030721
GTACATG207750.028.37545222
ACATGGG214600.027.58504524
GAGTACA224550.026.75350820
AGTACAT224400.026.45053521
TACATGG225650.026.39375123
CATGGGG156350.025.15510225
TACTTTT298950.024.91972223
ACTTTTT315200.023.73493224
GGTATCA644100.021.8886858
TCAACGC658650.021.4051512
CAACGCA661350.021.222513
GTATCAA669900.021.0523999
AACGCAG671250.020.94972214
ATCAACG677850.020.79221211
AAGCAGT677450.020.7646331
AGTGGTA683550.020.6550375
Produced by FastQC (version 0.11.5)