FastQCFastQC Report
Thu 26 May 2016
SRR805307_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805307_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22533109
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT665920.29552956939941133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA355800.033.3136613
TCAACGC356700.033.305312
AAGCAGT360400.032.6200071
ACGCAGA365050.032.4941815
AACGCAG367050.032.3661614
GGTATCA368250.032.346238
GTATCAA372150.032.031449
ATCAACG373800.031.82985711
GTGGTAT372600.031.8115926
AGTGGTA374600.031.7498665
TGGTATC377150.031.4337797
CGCAGAG378550.030.75881616
TATCAAC389300.030.57410810
AGAGTAC383900.030.08986719
CAGTGGT418450.028.4442584
CAGAGTA410450.028.28602618
GCAGTGG427300.027.865673
GCAGAGT422250.027.63943317
AGCAGTG430200.027.6307542
GTACATG286300.027.42752522
Produced by FastQC (version 0.11.5)