##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805307_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22533109
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.07733397996699	37.0	37.0	37.0	35.0	37.0
2	36.09223068152735	37.0	37.0	37.0	35.0	37.0
3	37.9582033264917	39.0	39.0	39.0	35.0	39.0
4	37.89578699503917	39.0	39.0	39.0	35.0	39.0
5	37.99702708578741	39.0	39.0	39.0	35.0	39.0
6	37.92854634484748	39.0	39.0	39.0	35.0	39.0
7	37.8734025118327	39.0	39.0	39.0	35.0	39.0
8	38.891970921544825	40.0	40.0	40.0	37.0	40.0
9	38.92369184385519	40.0	40.0	40.0	37.0	40.0
10	38.92848922889424	40.0	40.0	40.0	37.0	40.0
11	38.927739443323155	40.0	40.0	40.0	37.0	40.0
12	38.7568087031399	40.0	39.0	40.0	36.0	40.0
13	38.855076767258346	40.0	40.0	40.0	36.0	40.0
14	38.851513255450016	40.0	40.0	40.0	36.0	40.0
15	38.850823470476264	40.0	39.0	40.0	36.0	40.0
16	38.83781692086964	40.0	39.0	40.0	36.0	40.0
17	38.73230919887708	40.0	39.0	40.0	36.0	40.0
18	38.71271674050838	40.0	39.0	40.0	36.0	40.0
19	38.486946120040514	40.0	39.0	40.0	36.0	40.0
20	38.50535103700071	40.0	39.0	40.0	36.0	40.0
21	38.50569453154467	40.0	39.0	40.0	36.0	40.0
22	37.57546874690039	40.0	38.0	40.0	33.0	40.0
23	38.033506206356165	40.0	38.0	40.0	34.0	40.0
24	38.29116306143107	40.0	38.0	40.0	35.0	40.0
25	38.35298631005602	40.0	39.0	40.0	35.0	40.0
26	38.198962690856376	40.0	39.0	40.0	35.0	40.0
27	38.300465017943154	40.0	39.0	40.0	35.0	40.0
28	38.33993409431428	40.0	39.0	40.0	35.0	40.0
29	38.28617777511306	40.0	39.0	40.0	35.0	40.0
30	38.260325550282474	40.0	38.0	40.0	35.0	40.0
31	38.20118311237034	40.0	38.0	40.0	35.0	40.0
32	38.144540906450146	40.0	38.0	40.0	35.0	40.0
33	38.096302290110074	40.0	38.0	40.0	34.0	40.0
34	38.052353317067784	40.0	38.0	40.0	34.0	40.0
35	37.96556050032865	40.0	38.0	40.0	34.0	40.0
36	37.88508625241195	40.0	38.0	40.0	34.0	40.0
37	37.551779428218275	40.0	38.0	40.0	33.0	40.0
38	37.5344759571349	40.0	38.0	40.0	33.0	40.0
39	37.46535158552688	40.0	38.0	40.0	33.0	40.0
40	37.47887435329053	40.0	38.0	40.0	33.0	40.0
41	37.454738136668134	40.0	38.0	40.0	33.0	40.0
42	37.39308716786485	40.0	37.0	40.0	33.0	40.0
43	37.311720588579234	40.0	37.0	40.0	33.0	40.0
44	37.052579162511485	40.0	37.0	40.0	32.0	40.0
45	37.323483679060885	40.0	37.0	40.0	32.0	40.0
46	37.34249769971822	40.0	37.0	40.0	33.0	40.0
47	37.32110806369418	40.0	37.0	40.0	33.0	40.0
48	37.14652753865434	40.0	37.0	40.0	32.0	40.0
49	37.142258487277545	40.0	37.0	40.0	32.0	40.0
50	37.103209814500076	40.0	37.0	40.0	32.0	40.0
51	36.632601608592935	39.0	36.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	56.0
9	166.0
10	236.0
11	387.0
12	592.0
13	966.0
14	1385.0
15	2135.0
16	3196.0
17	4747.0
18	7334.0
19	11471.0
20	17605.0
21	25984.0
22	36053.0
23	48741.0
24	63665.0
25	80803.0
26	100811.0
27	122188.0
28	147349.0
29	177108.0
30	212734.0
31	254502.0
32	306852.0
33	377783.0
34	478797.0
35	658422.0
36	989750.0
37	1745378.0
38	4146907.0
39	1.2509E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.321591885078975	27.755086970022646	25.72421320111663	31.199107943781744
2	24.719873320632317	24.811911219175304	29.952413579502057	20.515801880690322
3	17.37329722232294	24.869533094612024	31.93037409973031	25.82679558333473
4	18.449744329555234	27.502995702901007	30.869548449794475	23.17771151774928
5	23.742977500352925	27.854491805813392	28.376102028353035	20.02642866548065
6	23.248616069801997	26.082721208156407	29.116683365797414	21.551979356244182
7	23.70808662044816	26.79821057981835	28.677707102024847	20.815995697708647
8	22.39210754272746	25.24509156725776	28.79753078015111	23.56527010986367
9	22.38557937122658	24.723326017727956	29.93610424553487	22.954990365510593
10	22.546888669468558	25.854607990402034	28.485017313855803	23.113486026273605
11	22.40331327558927	27.30449668529984	27.406204798459015	22.88598524065188
12	22.67695949103162	26.0166628581968	28.344082478809295	22.96229517196229
13	22.809604302717393	26.323673311126306	26.671801037309145	24.194921348847156
14	23.014866701261685	27.42041943701599	26.53911628439733	23.025597577324994
15	22.745312242531647	27.344078440307552	26.632889407316142	23.277719909844667
16	23.117036357477346	26.245885554452343	26.291498434592402	24.345579653477913
17	24.632823637430594	25.894269627861828	26.362363045419073	23.110543689288505
18	23.13288858630205	25.984767570245186	26.255617899864596	24.626725943588166
19	23.686655933719578	26.849730323498633	26.25445960430938	23.209154138472414
20	24.426052348124706	26.014417273710432	26.233459395239244	23.326070982925614
21	23.365492973029152	26.999589803608547	26.331306523214348	23.30361070014795
22	24.671429051357272	25.316901453767432	26.456575521824348	23.555093973050944
23	23.300153565138302	26.04070303836013	27.557373463200307	23.10176993330126
24	23.137708160911128	27.056159893426155	26.366849776477803	23.439282169184906
25	23.18355625049344	25.95449655881929	26.2568560778719	24.60509111281537
26	23.477412726313087	26.49987624876798	26.916401105590886	23.106309919328044
27	23.23208927804858	25.791660618159707	27.60353664467695	23.372713459114763
28	24.038950861152806	25.862494163588345	26.555310232600394	23.54324474265846
29	24.187936072203794	25.915855641580578	26.617130374685534	23.279077911530095
30	24.016499454203146	25.775488859526664	26.664624930363583	23.54338675590661
31	23.851923851253726	25.96758396721908	26.465557859769817	23.714934321757376
32	23.84624332132774	26.191654245315192	26.51547551649442	23.446626916862648
33	23.624928987828532	26.048851048472716	26.57075861125067	23.75546135244808
34	23.68566184098253	26.0573762812757	26.440230684545128	23.81673119319664
35	23.748453886234696	26.138141878246806	26.53632483648839	23.57707939903011
36	23.59190646971974	26.066447377501255	26.564146119383704	23.777500033395302
37	24.170832351629773	25.818021827347483	26.416332517629947	23.594813303392797
38	23.99036901654361	26.114416789977806	26.49709811460105	23.398116078877532
39	23.865432861483963	25.982397724166688	26.555208160578285	23.596961253771063
40	23.754964306079557	26.141337176330172	26.3294559130744	23.774242604515873
41	23.81287908384058	26.213293514001997	26.485062491820372	23.48876491033705
42	23.61415817053918	26.094020137212315	26.603576985315254	23.68824470693325
43	23.746328125426455	26.073587981134782	26.378783327236377	23.801300566202382
44	24.120572975526812	25.904911745645045	26.54579090706036	23.428724371767785
45	23.692087940461303	25.92670634132201	26.612923232209102	23.76828248600759
46	23.708099934190173	25.99796148858109	26.39689001637546	23.897048560853275
47	23.8053967608287	26.052325935138377	26.456579959738356	23.685697344294567
48	23.848830625192466	25.840051632466697	26.55773777156095	23.753379970779886
49	23.750122541900453	26.112796951366096	26.242770138821058	23.894310367912393
50	23.888931616138724	26.160899501262787	26.377052540774553	23.573116341823937
51	23.76594814324113	25.962187463789395	26.47910237331209	23.792762019657385
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12157.0
1	8240.0
2	4323.0
3	3709.0
4	3095.0
5	3092.0
6	3089.0
7	3551.0
8	4013.0
9	4853.0
10	5693.0
11	7901.5
12	10110.0
13	13860.0
14	17610.0
15	23506.5
16	29403.0
17	41450.0
18	53497.0
19	70973.5
20	88450.0
21	114668.0
22	140886.0
23	213618.5
24	286351.0
25	290043.0
26	336721.5
27	379708.0
28	425588.5
29	471469.0
30	517552.0
31	563635.0
32	615034.0
33	666433.0
34	729770.5
35	793108.0
36	876070.5
37	959033.0
38	1026279.5
39	1093526.0
40	1173195.0
41	1252864.0
42	1320914.5
43	1388965.0
44	1444840.5
45	1500716.0
46	1549702.0
47	1598688.0
48	1618219.0
49	1637750.0
50	1648157.5
51	1658565.0
52	1628568.0
53	1598571.0
54	1526554.0
55	1454537.0
56	1352785.0
57	1251033.0
58	1135821.0
59	1020609.0
60	904175.0
61	787741.0
62	681427.5
63	575114.0
64	489054.5
65	402995.0
66	320567.5
67	238140.0
68	192106.0
69	146072.0
70	110166.0
71	74260.0
72	53787.0
73	33314.0
74	24963.0
75	12469.5
76	8327.0
77	6270.5
78	4214.0
79	3229.0
80	2244.0
81	1814.0
82	1384.0
83	980.5
84	577.0
85	410.5
86	244.0
87	181.0
88	118.0
89	89.5
90	61.0
91	49.0
92	37.0
93	27.0
94	17.0
95	12.5
96	8.0
97	7.0
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.2533109E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.92220232449465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.67356142692	33.16159148266542
2	15.180446221776705	14.24599937988634
3	5.58157486454703	7.856993552507688
4	2.5394400660380514	4.76624482278262
5	1.4593553931139664	3.4238084509517988
6	0.9439810157879689	2.657620092797104
7	0.6147032570051078	2.019026141630365
8	0.44633656187632753	1.6754475568944291
9	0.3434781205028578	1.4505074877845026
>10	2.0056595789222547	17.55972732918383
>50	0.14477270980562917	4.630361415686538
>100	0.06390257962948236	5.0789782857197645
>500	0.0022866458571755275	0.7374670853532375
>1k	4.427310149573023E-4	0.26753225376554085
>5k	3.921813505326747E-5	0.11431330275295075
>10k+	1.96090675264256E-5	0.35438135963805345
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	66592	0.29552956939941133	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.686407321777035E-4	0.0	0.0	1.3313742014029223E-5	8.875828009352815E-6
2	1.9526821620576194E-4	4.437914004676407E-6	0.0	1.775165601870563E-5	1.3313742014029223E-5
3	1.9526821620576194E-4	1.3313742014029223E-5	0.0	2.2189570023382036E-5	2.2189570023382036E-5
4	1.9970613021043834E-4	1.3313742014029223E-5	0.0	2.6627484028058446E-5	3.9941226042087665E-5
5	8.432036608885175E-4	1.3313742014029223E-5	0.0	3.9941226042087665E-5	4.8817054051440485E-5
6	8.742690589212523E-4	1.3313742014029223E-5	0.0	4.437914004676407E-5	7.988245208417533E-5
7	9.008965429493107E-4	1.3313742014029223E-5	0.0	5.325496805611689E-5	8.875828009352814E-5
8	9.1421028496334E-4	1.3313742014029223E-5	0.0	6.21307960654697E-5	1.0207202210755737E-4
9	9.186481989680163E-4	1.3313742014029223E-5	0.0	6.656871007014612E-5	1.19823678126263E-4
10	9.674652530194569E-4	1.3313742014029223E-5	0.0	6.656871007014612E-5	1.286995061356158E-4
11	0.0010118443930662209	1.775165601870563E-5	0.0	6.656871007014612E-5	1.3313742014029224E-4
12	0.0010961647591550727	3.550331203741126E-5	0.0	6.656871007014612E-5	1.4645116215432143E-4
13	0.001264805491332776	3.550331203741126E-5	0.0	7.544453807949893E-5	1.5088907615899786E-4
14	0.001340250029412275	3.550331203741126E-5	0.0	7.544453807949893E-5	1.8639238819640911E-4
15	0.00148226327756192	3.550331203741126E-5	0.0	7.544453807949893E-5	1.9526821620576194E-4
16	0.0015399561596227134	3.550331203741126E-5	0.0	8.875828009352814E-5	2.0414404421511474E-4
17	0.0016065248696928594	3.9941226042087665E-5	0.0	9.763410810288097E-5	2.307715282431732E-4
18	0.0016686556657583291	3.9941226042087665E-5	0.0	9.763410810288097E-5	2.352094422478496E-4
19	0.0017307864618237988	4.8817054051440485E-5	0.0	1.0207202210755737E-4	2.485231842618788E-4
20	0.0017707276878658866	4.8817054051440485E-5	0.0	1.0650993611223378E-4	2.529610982665552E-4
21	0.0018151068279126507	4.8817054051440485E-5	0.0	1.0650993611223378E-4	2.6627484028058447E-4
22	0.0018639238819640911	5.325496805611689E-5	0.0	1.0650993611223378E-4	2.8402649629929007E-4
23	0.001917178850020208	5.325496805611689E-5	0.0	1.0650993611223378E-4	2.973402383133193E-4
24	0.001979309646085678	5.76928820607933E-5	0.0	1.0650993611223378E-4	3.106539803273485E-4
25	0.0020636300121745295	5.76928820607933E-5	0.0	1.1094785011691018E-4	3.150918943320249E-4
26	0.0021301987222446758	6.21307960654697E-5	0.0	1.19823678126263E-4	3.150918943320249E-4
27	0.0022189570023382035	6.21307960654697E-5	0.0	1.19823678126263E-4	3.150918943320249E-4
28	0.002321029024445761	6.656871007014612E-5	0.0	1.3757533414496864E-4	3.284056363460542E-4
29	0.0024586043585907297	6.656871007014612E-5	0.0	1.4201324814964504E-4	3.284056363460542E-4
30	0.002578428036716993	6.656871007014612E-5	0.0	1.4201324814964504E-4	3.417193783600834E-4
31	0.0026627484028058446	7.100662407482252E-5	0.0	1.5088907615899786E-4	3.5947103437878903E-4
32	0.002809199564960166	7.100662407482252E-5	0.0	1.5088907615899786E-4	3.6834686238814183E-4
33	0.0029157095010723996	7.988245208417533E-5	0.0	1.5088907615899786E-4	3.905364324115239E-4
34	0.003071036491236074	7.988245208417533E-5	0.0	1.5532699016367426E-4	3.949743464162003E-4
35	0.003204173911376366	7.988245208417533E-5	0.0	1.686407321777035E-4	4.3047765845361153E-4
36	0.003386128385568099	7.988245208417533E-5	0.0	1.686407321777035E-4	4.3935348646296433E-4
37	0.0035370174617270966	7.988245208417533E-5	0.0	1.686407321777035E-4	4.526672284769936E-4
38	0.0038032923020076812	7.988245208417533E-5	0.0	1.775165601870563E-4	4.970463685237576E-4
39	0.003954181378166679	8.432036608885174E-5	0.0	1.8195447419173271E-4	5.192359385471397E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGCA	35580	0.0	33.31366	13
TCAACGC	35670	0.0	33.3053	12
AAGCAGT	36040	0.0	32.620007	1
ACGCAGA	36505	0.0	32.49418	15
AACGCAG	36705	0.0	32.36616	14
GGTATCA	36825	0.0	32.34623	8
GTATCAA	37215	0.0	32.03144	9
ATCAACG	37380	0.0	31.829857	11
GTGGTAT	37260	0.0	31.811592	6
AGTGGTA	37460	0.0	31.749866	5
TGGTATC	37715	0.0	31.433779	7
CGCAGAG	37855	0.0	30.758816	16
TATCAAC	38930	0.0	30.574108	10
AGAGTAC	38390	0.0	30.089867	19
CAGTGGT	41845	0.0	28.444258	4
CAGAGTA	41045	0.0	28.286026	18
GCAGTGG	42730	0.0	27.86567	3
GCAGAGT	42225	0.0	27.639433	17
AGCAGTG	43020	0.0	27.630754	2
GTACATG	28630	0.0	27.427525	22
>>END_MODULE