FastQCFastQC Report
Thu 26 May 2016
SRR805306_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805306_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28621012
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11650484.070603792765958No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT616990.21557239136058504No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG508640.1777155888128624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3087150.043.3105771
TCAACGC3114650.043.1976312
CAACGCA3107500.043.16814413
GGTATCA3124750.043.0637668
AGTGGTA3123900.043.06185
GTATCAA3129200.043.02059
TGGTATC3119300.043.0084467
GTGGTAT3118050.043.004766
ACGCAGA3121700.042.9984515
AACGCAG3127200.042.94009414
ATCAACG3138150.042.8676911
CGCAGAG3105750.042.7251916
TATCAAC3151600.042.72472810
AGAGTAC3080050.042.5601119
AGCAGTG3159750.042.330412
CAGTGGT3160450.042.315334
GCAGTGG3169500.042.2477533
CAGAGTA3123500.042.1510318
GAGTACT1516700.042.10127320
GCAGAGT3147750.041.956417