FastQCFastQC Report
Thu 26 May 2016
SRR805305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24603623
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8013503.2570406399090084No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT735640.29899661525459076No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG548450.22291432444725723No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC484760.19702789300583903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA3398250.043.87574813
TCAACGC3389750.043.8689412
GTATCAA3391050.043.787769
GGTATCA3398500.043.7036938
ACGCAGA3412200.043.70230515
AACGCAG3412100.043.70028714
GTGGTAT3401050.043.6795276
AAGCAGT3402950.043.6710051
AGTGGTA3402700.043.6682625
ATCAACG3403150.043.6624911
TGGTATC3407250.043.5993847
CGCAGAG3404400.043.5023816
AGAGTAC3363800.043.4695919
TATCAAC3416900.043.44859310
CAGAGTA3404750.043.0881918
CAGTGGT3462950.042.9695784
GCAGAGT3435300.042.95454417
AGCAGTG3469100.042.888862
GCAGTGG3476100.042.8859943
GTACATG2266350.042.84267822