##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805305_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24603623
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.32789735885646	37.0	37.0	37.0	35.0	37.0
2	36.257091282857	37.0	37.0	37.0	35.0	37.0
3	38.058376565109945	39.0	39.0	39.0	35.0	39.0
4	38.00006267369647	39.0	38.0	39.0	35.0	39.0
5	37.966015167766145	39.0	38.0	39.0	35.0	39.0
6	37.995081740603815	39.0	38.0	39.0	35.0	39.0
7	37.83997694160734	39.0	38.0	39.0	35.0	39.0
8	38.78613296098709	40.0	39.0	40.0	36.0	40.0
9	38.758122289550606	40.0	39.0	40.0	36.0	40.0
10	38.559210771519304	40.0	38.0	40.0	36.0	40.0
11	38.56308422544111	40.0	38.0	40.0	36.0	40.0
12	38.51450889976651	40.0	38.0	40.0	36.0	40.0
13	38.65778040087836	40.0	39.0	40.0	36.0	40.0
14	38.55603888094042	40.0	38.0	40.0	36.0	40.0
15	38.5097607779147	40.0	38.0	40.0	36.0	40.0
16	38.475293008676	40.0	38.0	40.0	36.0	40.0
17	38.280800352045716	40.0	38.0	40.0	35.0	40.0
18	38.275895708530406	40.0	38.0	40.0	35.0	40.0
19	38.214305023288645	40.0	38.0	40.0	35.0	40.0
20	38.004088625484144	40.0	38.0	40.0	34.0	40.0
21	37.66704915776022	39.0	37.0	40.0	34.0	40.0
22	37.329211555550174	39.0	37.0	40.0	33.0	40.0
23	37.254106681767965	39.0	37.0	40.0	33.0	40.0
24	37.27986203495315	39.0	37.0	40.0	33.0	40.0
25	37.13832048231271	38.0	36.0	40.0	33.0	40.0
26	37.14052093059628	38.0	36.0	40.0	33.0	40.0
27	37.03523903776285	38.0	36.0	40.0	33.0	40.0
28	36.812490461262556	38.0	36.0	40.0	32.0	40.0
29	36.524427073199746	38.0	36.0	40.0	31.0	40.0
30	36.29564243444959	38.0	35.0	40.0	31.0	40.0
31	35.91254479065949	38.0	35.0	40.0	30.0	40.0
32	35.81893122000772	38.0	35.0	39.0	30.0	40.0
33	35.59080372024884	38.0	34.0	39.0	30.0	40.0
34	35.38739652286169	37.0	34.0	39.0	30.0	40.0
35	35.26466378549208	37.0	34.0	39.0	29.0	40.0
36	34.947989326612586	37.0	33.0	39.0	29.0	40.0
37	34.68159396687228	37.0	33.0	38.0	29.0	40.0
38	34.36593220437494	36.0	33.0	38.0	28.0	40.0
39	34.10268804720346	36.0	32.0	38.0	27.0	39.0
40	33.82100591445414	35.0	32.0	38.0	27.0	39.0
41	33.71132962816086	35.0	32.0	38.0	26.0	39.0
42	33.58336079202644	35.0	32.0	38.0	26.0	39.0
43	33.51105058795609	35.0	32.0	38.0	26.0	39.0
44	33.25509661727462	35.0	31.0	38.0	26.0	39.0
45	32.951154226351136	35.0	31.0	38.0	25.0	39.0
46	32.68487356516559	34.0	31.0	37.0	24.0	39.0
47	32.39309840668588	34.0	30.0	37.0	24.0	39.0
48	32.04470004275387	34.0	30.0	37.0	24.0	38.0
49	31.52561051679259	33.0	29.0	37.0	23.0	38.0
50	31.312972280545836	33.0	29.0	36.0	22.0	38.0
51	32.13822574016843	34.0	30.0	37.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	35.0
8	211.0
9	409.0
10	566.0
11	578.0
12	629.0
13	604.0
14	680.0
15	964.0
16	1386.0
17	2740.0
18	5341.0
19	10547.0
20	18766.0
21	31531.0
22	49206.0
23	71724.0
24	98790.0
25	130517.0
26	168566.0
27	210993.0
28	260080.0
29	315938.0
30	383658.0
31	473938.0
32	600648.0
33	806433.0
34	1188670.0
35	2035276.0
36	4410047.0
37	8619784.0
38	4579084.0
39	125284.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.45359990274603	38.42482873355684	27.846906937242533	20.274664426454592
2	26.692491589551672	44.05889327762826	11.805834449666214	17.442780683153856
3	38.18518110117359	24.384225851615433	16.896365222308926	20.53422782490205
4	21.99901616115643	22.0171273149487	18.53417685679869	37.44967966709618
5	20.25402925414684	35.97341741092359	21.892808225845435	21.879745109084137
6	34.96590725682962	22.149977667923135	20.411717412512782	22.472397662734465
7	21.93411515043943	21.39653985106177	34.88965832389807	21.77968667460073
8	34.41459820775176	23.331031368835394	21.58793442738088	20.666435996031968
9	34.635459988961784	22.802629515173436	21.593714063981555	20.968196431883225
10	20.850689347662335	23.0499670719227	35.336405536696766	20.762938043718197
11	20.782353883409773	36.36629857318168	22.01992771552385	20.831419827884698
12	20.72156608805134	22.670169348636175	35.63729211750643	20.970972445806048
13	20.65260876416453	22.817147702190038	22.347745289382786	34.182498244262646
14	20.98655551664078	36.38252789030298	21.990480832843197	20.640435760213037
15	20.769063157893452	36.16105644278487	22.032625032500295	21.037255366821384
16	20.96547732014915	22.43320018356646	22.287599675868876	34.313722820415514
17	34.425787616726204	22.450417160106866	22.1647641081153	20.959031115051634
18	20.896454965189477	22.503730446528138	22.16775147302493	34.432063115257456
19	20.932734174962768	35.938829009044724	22.2325224215962	20.895914394396307
20	34.5693762256071	22.627886957949244	22.124221298627443	20.67851551781622
21	21.053224559651234	35.84618818130972	22.13031389726627	20.970273361772776
22	34.2502077844389	22.317457067196976	22.497024117139173	20.935311031224956
23	21.09785213340328	22.529149467133358	35.535217719764276	20.837780679699083
24	21.05741093496677	35.659378295627434	22.140954606563433	21.14225616284236
25	20.805899196228133	22.627017167349702	22.261294606895905	34.30578902952626
26	21.129554781423856	31.487350460539897	26.468951341028106	20.914143417008138
27	21.21440813818355	22.532880624938855	35.31524198692201	20.937469249955587
28	30.005206956715273	22.41681641764711	26.598350982698765	20.979625642938846
29	30.166536855161535	22.586076042540565	26.328329774846576	20.919057327451327
30	30.021363113879612	22.49947091125563	26.405676107132674	21.073489867732082
31	26.895022737098518	23.726176425317526	27.260773748646695	22.118027088937268
32	24.882437029700867	25.173670560632473	27.464003980226813	22.479888429439843
33	24.386473488071246	24.313736233074295	28.135535160817575	23.164255118036884
34	23.566899070108498	24.06846341288842	28.699277338138373	23.665360178864713
35	23.475294675097242	24.331054820666047	28.835932008875275	23.357718495361436
36	23.601511858639682	24.358611737791623	28.84292284920802	23.19695355436067
37	24.164896365059732	24.09478880407166	28.74324647227768	22.997068358590926
38	24.288609852296954	24.329485946033234	28.60587645973928	22.776027741930527
39	24.20663818495349	24.242795461465168	28.602832192640896	22.947734160940445
40	23.66668518697429	24.720505593830634	28.339915629498957	23.27289358969612
41	24.03574465435436	25.080505419872512	28.045625638142806	22.838124287630322
42	23.903300745585316	24.987576016751678	28.319764125795622	22.789359111867384
43	23.75327406049101	24.615143062466856	28.96117779076683	22.670405086275302
44	24.169038031512677	24.363692290359026	28.348276186803872	23.118993491324428
45	24.173504853329934	23.988312615585112	28.40146347552147	23.436719055563486
46	23.83591636077337	24.01559721509308	28.232724099210916	23.915762324922635
47	23.74993715356474	24.262325105534256	28.287329878205338	23.70040786269567
48	23.5750929852892	24.20578871656422	28.31565903932116	23.903459258825418
49	23.37146850282985	24.964107928332343	28.113672527009538	23.55075104182827
50	24.115074434362775	24.7171036558315	27.89219701504937	23.27562489475635
51	23.768328753858732	24.596991264254047	28.22322143368885	23.411458548198368
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3711.0
1	2331.5
2	952.0
3	805.5
4	659.0
5	712.0
6	765.0
7	903.5
8	1042.0
9	1408.5
10	1775.0
11	2827.5
12	3880.0
13	5547.0
14	7214.0
15	10198.0
16	13182.0
17	19615.0
18	26048.0
19	36099.0
20	46150.0
21	65482.0
22	84814.0
23	532146.0
24	979478.0
25	642706.5
26	299962.5
27	293990.0
28	321278.5
29	348567.0
30	379971.0
31	411375.0
32	456745.5
33	502116.0
34	565247.5
35	628379.0
36	713376.0
37	798373.0
38	879113.5
39	959854.0
40	1050432.5
41	1141011.0
42	1228278.0
43	1315545.0
44	1421108.0
45	1526671.0
46	1629597.0
47	1732523.0
48	1774059.0
49	1815595.0
50	1866238.0
51	1916881.0
52	1930489.0
53	1944097.0
54	1922779.0
55	1901461.0
56	1784290.0
57	1667119.0
58	1490099.5
59	1313080.0
60	1152655.5
61	992231.0
62	858370.0
63	724509.0
64	613723.5
65	502938.0
66	399750.0
67	296562.0
68	243270.0
69	189978.0
70	143667.0
71	97356.0
72	73977.0
73	50598.0
74	38183.0
75	20033.0
76	14298.0
77	10863.0
78	7428.0
79	6073.5
80	4719.0
81	3969.5
82	3220.0
83	2275.5
84	1331.0
85	827.5
86	324.0
87	199.0
88	74.0
89	50.0
90	26.0
91	20.0
92	14.0
93	9.0
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.4603623E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.999684971533874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.990091083563	27.685911883594528
2	14.20675808463746	11.081181795353059
3	5.12495498176977	5.996148893469424
4	2.433448106698087	3.7961483822320377
5	1.4828885726993972	2.8916093591581995
6	0.9730290073873995	2.2768694853763733
7	0.6627592846845675	1.8093182320260086
8	0.5072000687646542	1.5824514319489464
9	0.39884362507410104	1.3999298157715054
>10	2.871204957847328	21.638436532013408
>50	0.24155682091299072	6.439282328664079
>100	0.10217149564224555	6.717208456636928
>500	0.0036063793252392494	0.9280386172392208
>1k	0.0012293334156409134	0.8801625830710081
>5k	1.5061525393102537E-4	0.39016017700553063
>10k+	1.0758232423608171E-4	4.487142026439506
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	801350	3.2570406399090084	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT	73564	0.29899661525459076	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG	54845	0.22291432444725723	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC	48476	0.19702789300583903	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.941772518624595E-5	8.12888410784054E-6	0.0	0.0	1.625776821568108E-5
2	9.754660929408649E-5	8.12888410784054E-6	0.0	4.06444205392027E-6	2.0322210269601352E-5
3	1.1380437750976757E-4	8.12888410784054E-6	0.0	8.12888410784054E-6	2.4386652323521622E-5
4	1.1786881956368783E-4	8.12888410784054E-6	0.0	8.12888410784054E-6	2.8451094377441892E-5
5	1.2193326161760811E-4	8.12888410784054E-6	0.0	1.2193326161760811E-5	4.4708862593122974E-5
6	1.259977036715284E-4	8.12888410784054E-6	0.0	1.2193326161760811E-5	5.2837746700963515E-5
7	1.259977036715284E-4	8.12888410784054E-6	0.0	2.4386652323521622E-5	6.909551491664459E-5
8	1.259977036715284E-4	8.12888410784054E-6	0.0	2.8451094377441892E-5	7.315995697056487E-5
9	1.259977036715284E-4	8.12888410784054E-6	0.0	2.8451094377441892E-5	9.754660929408649E-5
10	1.3006214572544865E-4	1.2193326161760811E-5	0.0	2.8451094377441892E-5	9.754660929408649E-5
11	1.3819102983328918E-4	1.2193326161760811E-5	0.0	3.251553643136216E-5	1.1380437750976757E-4
12	1.3819102983328918E-4	1.625776821568108E-5	0.0	3.657997848528243E-5	1.2193326161760811E-4
13	1.4631991394112973E-4	1.625776821568108E-5	0.0	4.0644420539202704E-5	1.259977036715284E-4
14	1.5851324010289055E-4	1.625776821568108E-5	0.0	4.4708862593122974E-5	1.3819102983328918E-4
15	1.7070656626465134E-4	1.625776821568108E-5	0.0	4.4708862593122974E-5	1.4225547188720947E-4
16	1.788354503724919E-4	1.625776821568108E-5	0.0	4.4708862593122974E-5	1.4631991394112973E-4
17	2.032221026960135E-4	1.625776821568108E-5	0.0	4.8773304647043244E-5	1.6664212421073108E-4
18	2.1947987091169459E-4	2.0322210269601352E-5	0.0	4.8773304647043244E-5	1.8696433448033242E-4
19	2.519954073430568E-4	2.0322210269601352E-5	0.0	4.8773304647043244E-5	1.8696433448033242E-4
20	3.048331540440203E-4	2.0322210269601352E-5	0.0	4.8773304647043244E-5	2.0728654474993377E-4
21	3.7799311101458514E-4	2.4386652323521622E-5	0.0	5.2837746700963515E-5	2.0728654474993377E-4
22	4.2676641566162835E-4	2.8451094377441892E-5	0.0	5.2837746700963515E-5	2.3980208118129593E-4
23	4.8773304647043244E-4	2.8451094377441892E-5	0.0	5.2837746700963515E-5	2.519954073430568E-4
24	5.40570793171396E-4	2.8451094377441892E-5	0.0	5.2837746700963515E-5	2.7638205966657836E-4
25	6.015374239802E-4	2.8451094377441892E-5	0.0	5.2837746700963515E-5	3.007687119901E-4
26	6.990840332742865E-4	2.8451094377441892E-5	0.0	5.2837746700963515E-5	3.2109092225970134E-4
27	7.722439902448513E-4	2.8451094377441892E-5	0.0	5.2837746700963515E-5	3.3328424842146216E-4
28	8.413395051614959E-4	2.8451094377441892E-5	0.0	5.2837746700963515E-5	3.6173534279890403E-4
29	9.592083247251837E-4	2.8451094377441892E-5	0.0	6.0966630808804055E-5	3.7392866896066485E-4
30	0.0010648838181271107	2.8451094377441892E-5	0.0	6.0966630808804055E-5	3.8205755306850537E-4
31	0.0011908815217986392	2.8451094377441892E-5	0.0	6.0966630808804055E-5	3.8612199512242566E-4
32	0.0013778458562789715	2.8451094377441892E-5	0.0	6.909551491664459E-5	3.983153212841865E-4
33	0.0015322946543279419	2.8451094377441892E-5	0.0	6.909551491664459E-5	4.06444205392027E-4
34	0.00165422791594555	2.8451094377441892E-5	0.0	6.909551491664459E-5	4.105086474459473E-4
35	0.0018289989242641215	2.8451094377441892E-5	0.0	6.909551491664459E-5	4.227019736077081E-4
36	0.0020037699325826932	2.8451094377441892E-5	0.0	6.909551491664459E-5	4.3083085771554864E-4
37	0.002227314245548308	2.8451094377441892E-5	0.0	6.909551491664459E-5	4.4302418387730946E-4
38	0.002467116326729604	3.657997848528243E-5	0.0	6.909551491664459E-5	4.470886259312297E-4
39	0.0026418873350481757	3.657997848528243E-5	0.0	6.909551491664459E-5	4.674108362008311E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGCA	339825	0.0	43.875748	13
TCAACGC	338975	0.0	43.86894	12
GTATCAA	339105	0.0	43.78776	9
GGTATCA	339850	0.0	43.703693	8
ACGCAGA	341220	0.0	43.702305	15
AACGCAG	341210	0.0	43.700287	14
GTGGTAT	340105	0.0	43.679527	6
AAGCAGT	340295	0.0	43.671005	1
AGTGGTA	340270	0.0	43.668262	5
ATCAACG	340315	0.0	43.66249	11
TGGTATC	340725	0.0	43.599384	7
CGCAGAG	340440	0.0	43.50238	16
AGAGTAC	336380	0.0	43.46959	19
TATCAAC	341690	0.0	43.448593	10
CAGAGTA	340475	0.0	43.08819	18
CAGTGGT	346295	0.0	42.969578	4
GCAGAGT	343530	0.0	42.954544	17
AGCAGTG	346910	0.0	42.88886	2
GCAGTGG	347610	0.0	42.885994	3
GTACATG	226635	0.0	42.842678	22
>>END_MODULE