FastQCFastQC Report
Thu 26 May 2016
SRR805304_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805304_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22836239
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4783102.0945217818047883No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT425460.18630913785759556No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG335400.1468718207056775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC310210.13584110763598156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1775100.043.24953512
CAACGCA1782450.043.16839613
GTATCAA1784950.042.947849
AACGCAG1792550.042.90884414
ATCAACG1789200.042.88103511
GGTATCA1788100.042.8784758
ACGCAGA1794550.042.86353315
GTGGTAT1791950.042.8177386
AGTGGTA1798050.042.683745
TGGTATC1800700.042.605937
TATCAAC1801200.042.56162610
CGCAGAG1799400.042.4791616
AAGCAGT1815800.042.3693431
AGAGTAC1784350.042.32171219
CAGAGTA1811150.041.84330418
GCAGAGT1838850.041.4638217
CAGTGGT1855800.041.4451984
GTACATG1141950.041.2879322
AGCAGTG1866550.041.1956562
GCAGTGG1871850.041.166763