##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805304_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22836239
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.7132216474	35.0	35.0	35.0	33.0	37.0
2	35.5616412142122	36.0	35.0	37.0	35.0	37.0
3	37.922297712858935	39.0	38.0	39.0	35.0	39.0
4	37.9366511271843	39.0	38.0	39.0	35.0	39.0
5	37.930732245357916	39.0	38.0	39.0	35.0	39.0
6	37.953750790574574	39.0	38.0	39.0	35.0	39.0
7	37.810655642551296	39.0	38.0	39.0	35.0	39.0
8	38.77960626528738	40.0	39.0	40.0	36.0	40.0
9	38.761037577159705	40.0	39.0	40.0	36.0	40.0
10	38.63453185088841	40.0	38.0	40.0	36.0	40.0
11	38.62570504714021	40.0	38.0	40.0	36.0	40.0
12	38.52099130684348	40.0	38.0	40.0	36.0	40.0
13	38.64570374307258	40.0	39.0	40.0	36.0	40.0
14	38.5423057185555	40.0	38.0	40.0	36.0	40.0
15	38.49952218489218	40.0	38.0	40.0	36.0	40.0
16	38.45736922791883	40.0	38.0	40.0	36.0	40.0
17	38.32207010970589	40.0	38.0	40.0	35.0	40.0
18	38.266668561316074	40.0	38.0	40.0	35.0	40.0
19	38.20025915826157	40.0	38.0	40.0	35.0	40.0
20	38.00564208493351	40.0	38.0	40.0	34.0	40.0
21	37.76811133391974	40.0	37.0	40.0	34.0	40.0
22	37.281013567952236	39.0	37.0	40.0	33.0	40.0
23	37.31467410198326	39.0	37.0	40.0	33.0	40.0
24	37.288811655894825	39.0	37.0	40.0	33.0	40.0
25	37.18081304894383	38.0	36.0	40.0	33.0	40.0
26	37.11836848440761	38.0	36.0	40.0	33.0	40.0
27	36.98916056185959	38.0	36.0	40.0	33.0	40.0
28	36.804701991426874	38.0	36.0	40.0	32.0	40.0
29	36.609465507871064	38.0	36.0	40.0	31.0	40.0
30	36.36565237384317	38.0	35.0	40.0	31.0	40.0
31	35.98236710519626	38.0	35.0	40.0	30.0	40.0
32	35.89234212341183	38.0	35.0	39.0	30.0	40.0
33	35.71112419168498	38.0	34.0	39.0	30.0	40.0
34	35.499017504589965	38.0	34.0	39.0	30.0	40.0
35	35.365222049042316	37.0	34.0	39.0	30.0	40.0
36	35.055086522785125	37.0	33.0	39.0	29.0	40.0
37	34.77479789907611	36.0	33.0	38.0	29.0	40.0
38	34.530844593104845	36.0	33.0	38.0	28.0	40.0
39	34.26146731079492	36.0	33.0	38.0	27.0	39.0
40	33.972312297134394	36.0	32.0	38.0	27.0	39.0
41	33.88021197360914	35.0	32.0	38.0	27.0	39.0
42	33.756719440534845	35.0	32.0	38.0	27.0	39.0
43	33.6523870677654	35.0	32.0	38.0	27.0	39.0
44	33.388169610591305	35.0	31.0	38.0	26.0	39.0
45	33.091081066369995	35.0	31.0	38.0	26.0	39.0
46	32.807493957301816	34.0	31.0	37.0	24.0	39.0
47	32.530742737453394	34.0	30.0	37.0	24.0	39.0
48	32.178367593718036	34.0	30.0	37.0	24.0	38.0
49	31.667139672167558	34.0	29.0	37.0	23.0	38.0
50	31.45274224008603	33.0	29.0	36.0	23.0	38.0
51	32.511726471245986	34.0	31.0	37.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	37.0
8	219.0
9	460.0
10	577.0
11	661.0
12	695.0
13	699.0
14	728.0
15	922.0
16	1455.0
17	2626.0
18	4839.0
19	9244.0
20	16453.0
21	27437.0
22	42785.0
23	61553.0
24	84881.0
25	112968.0
26	145908.0
27	184345.0
28	230638.0
29	283841.0
30	349328.0
31	436445.0
32	561950.0
33	761882.0
34	1125804.0
35	1933414.0
36	4173648.0
37	8083736.0
38	4106812.0
39	89249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.621279756268095	43.64949499784093	21.1084846326928	21.620740613198173
2	27.93115801599379	40.32403934816061	17.205350670922652	14.539451964922945
3	33.69731329226323	24.23882496587989	21.332514517824062	20.73134722403282
4	21.83325371572788	25.2109289975464	21.161120270286187	31.794697016439528
5	20.418484847701933	33.17574754757121	24.662874652870816	21.74289295185604
6	29.49325850022852	23.27010590491718	23.523391921060206	23.713243673794096
7	23.489126208567008	23.660463529042588	30.677026107495198	22.17338415489521
8	28.570234354264727	24.39740186639315	24.773786086228995	22.25857769311313
9	29.6203284612672	23.570763994894257	24.566663538597577	22.24224400524097
10	22.3118351493869	24.626577082154377	31.042051188901993	22.019536579556732
11	21.895085263383343	32.03821785189759	24.072983296417593	21.99371358830147
12	22.16450353317812	24.076394541150144	31.414060782951168	22.345041142720568
13	22.14217498774645	24.64641397385971	23.734521258075816	29.47688978031803
14	22.24242792344221	32.15588171064421	23.696835542840482	21.9048548230731
15	22.192191980474544	31.758981853360353	23.611773374766308	22.43705279139879
16	22.44509264419592	24.250985462185785	23.68838844259775	29.615533451020546
17	29.85062470225504	24.085235751824108	23.874776402541592	22.189363143379257
18	22.327787863842204	23.853306142049046	23.705821260672565	30.113084733436185
19	22.42763793109715	31.708054027635633	23.59735331198802	22.266954729279195
20	30.01285369276438	24.25864434156605	23.72767687358676	22.000825092082806
21	22.65248231111962	31.349400398200423	23.560258762399535	22.437858528280426
22	29.82751669397049	24.100579784613394	23.755080685571738	22.31682283584438
23	22.398158470841018	24.171335744033858	31.37117718902837	22.059328596096755
24	22.53130649053025	31.205917927203338	23.65582178396364	22.606953798302776
25	22.305704542678853	24.28940685022608	23.549731634880857	29.855156972214207
26	22.412420889446814	28.864805627581667	26.55131609018455	22.171457392786962
27	22.642922943659855	23.815594152784968	31.02358492569639	22.517897977858787
28	27.22421586146475	24.110787244782294	26.31797644086664	22.34702045288631
29	27.29684165593117	24.119124869905242	26.35990103274011	22.224132441423475
30	27.355108693686383	23.76798561269218	26.304734330377254	22.572171363244184
31	25.95840759943001	24.5652666360691	26.5130786203455	22.963247144155392
32	24.698471582820623	25.390783482341376	26.913573640563143	22.99717129427486
33	24.526166502286127	24.79091237396841	27.004162112684142	23.678759011061324
34	23.984860203994188	24.930423087619637	27.24607585338374	23.83864085500244
35	23.81652250180076	25.049821032263676	27.592205529115365	23.5414509368202
36	24.13283553390731	24.76022868739463	27.455098889094653	23.651836889603405
37	24.325590566817944	24.926644006484604	27.29916252847065	23.448602898226806
38	24.30610399549593	24.989999447807495	27.58230897828666	23.121587578409912
39	24.43703185975589	24.71128017183565	27.38926493105979	23.46242303734866
40	23.99920582369102	25.343871204010433	27.07238262833035	23.5845403439682
41	24.12792229053129	25.486254544804858	27.183083869458542	23.202739295205312
42	24.210847504267228	25.111486177737063	27.23148500941858	23.446181308577128
43	24.017689602915787	25.27314589762351	27.463151878906157	23.24601262055455
44	24.19169811631416	25.025999246198115	27.415753531043357	23.366549106444367
45	24.4213812966312	24.543682521451977	27.200604267629185	23.834331914287638
46	24.069042192105275	24.882845200560393	27.01926967921469	24.028842928119644
47	23.868904157116237	24.93661937939956	27.348951812949586	23.845524650534617
48	23.993478085423785	24.584499225113206	27.26236575120798	24.159656938255026
49	23.844386109288838	25.382634154424466	26.98964133279565	23.783338403491047
50	24.15608367034519	25.212409101165917	27.171974334302597	23.459532894186296
51	24.09906902796034	24.946240052926402	27.12793030411006	23.8267606150032
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5455.0
1	3607.5
2	1760.0
3	1544.0
4	1328.0
5	1385.0
6	1442.0
7	1621.5
8	1801.0
9	2285.0
10	2769.0
11	4189.5
12	5610.0
13	7781.0
14	9952.0
15	13427.5
16	16903.0
17	23897.5
18	30892.0
19	42376.5
20	53861.0
21	72791.0
22	91721.0
23	369149.5
24	646578.0
25	452636.5
26	266184.0
27	273673.0
28	306182.0
29	338691.0
30	377062.0
31	415433.0
32	462205.0
33	508977.0
34	572277.0
35	635577.0
36	713716.0
37	791855.0
38	868486.5
39	945118.0
40	1032063.5
41	1119009.0
42	1202438.0
43	1285867.0
44	1382340.0
45	1478813.0
46	1568736.0
47	1658659.0
48	1689470.0
49	1720281.0
50	1757475.0
51	1794669.0
52	1791275.5
53	1787882.0
54	1747518.0
55	1707154.0
56	1596905.5
57	1486657.0
58	1336752.5
59	1186848.0
60	1043240.0
61	899632.0
62	773607.5
63	647583.0
64	543685.5
65	439788.0
66	348850.5
67	257913.0
68	208828.5
69	159744.0
70	120403.0
71	81062.0
72	61195.5
73	41329.0
74	31174.5
75	16010.5
76	11001.0
77	8319.5
78	5638.0
79	4651.5
80	3665.0
81	3133.5
82	2602.0
83	1769.0
84	936.0
85	601.0
86	266.0
87	175.5
88	85.0
89	56.0
90	27.0
91	17.0
92	7.0
93	5.0
94	3.0
95	3.0
96	3.0
97	2.5
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.2836239E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.83145931364272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.35865067661027	30.400908751810007
2	15.252869499200091	13.371110577409818
3	5.765311455828803	7.581060435199155
4	2.7897570906032616	4.891164976468927
5	1.5706419695507785	3.4421764792332303
6	1.0405843414019396	2.7366198133543693
7	0.704061875689605	2.1602041621003134
8	0.5105493783946184	1.790249944536744
9	0.4005443167832502	1.580079773195627
>10	2.3926484277780244	19.132525234162365
>50	0.15784425890975165	4.681488986842241
>100	0.053229718424213875	3.985982413346022
>500	0.0024080734705450666	0.7013515459670754
>1k	7.855064700567678E-4	0.6490290973896035
>5k	6.186048252208252E-5	0.2035700374681333
>10k+	5.155040210161062E-5	2.692477771516448
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	478310	2.0945217818047883	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT	42546	0.18630913785759556	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG	33540	0.1468718207056775	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC	31021	0.13584110763598156	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.692706228902229E-5	0.0	0.0	0.0	1.3137014374389758E-5
2	7.006407666341204E-5	0.0	0.0	0.0	1.3137014374389758E-5
3	7.006407666341204E-5	0.0	4.3790047914632525E-6	0.0	1.3137014374389758E-5
4	8.32010910378018E-5	0.0	4.3790047914632525E-6	0.0	1.3137014374389758E-5
5	8.32010910378018E-5	4.3790047914632525E-6	4.3790047914632525E-6	0.0	1.751601916585301E-5
6	8.758009582926505E-5	8.758009582926505E-6	8.758009582926505E-6	4.3790047914632525E-6	1.751601916585301E-5
7	8.758009582926505E-5	8.758009582926505E-6	8.758009582926505E-6	8.758009582926505E-6	2.1895023957316263E-5
8	8.758009582926505E-5	8.758009582926505E-6	8.758009582926505E-6	8.758009582926505E-6	2.1895023957316263E-5
9	8.758009582926505E-5	8.758009582926505E-6	1.3137014374389758E-5	1.3137014374389758E-5	2.1895023957316263E-5
10	9.195910062072831E-5	8.758009582926505E-6	2.6274028748779517E-5	1.3137014374389758E-5	3.065303354024277E-5
11	9.633810541219156E-5	8.758009582926505E-6	2.6274028748779517E-5	1.3137014374389758E-5	3.065303354024277E-5
12	9.633810541219156E-5	1.3137014374389758E-5	3.503203833170602E-5	1.3137014374389758E-5	4.816905270609578E-5
13	9.633810541219156E-5	1.3137014374389758E-5	3.503203833170602E-5	1.751601916585301E-5	5.2548057497559034E-5
14	1.0509611499511807E-4	1.3137014374389758E-5	3.503203833170602E-5	2.1895023957316263E-5	5.2548057497559034E-5
15	1.0947511978658132E-4	1.3137014374389758E-5	3.9411043123169274E-5	2.1895023957316263E-5	5.2548057497559034E-5
16	1.2699113895243432E-4	1.3137014374389758E-5	3.9411043123169274E-5	2.1895023957316263E-5	7.006407666341204E-5
17	1.664021820756036E-4	1.751601916585301E-5	3.9411043123169274E-5	2.1895023957316263E-5	7.44430814548753E-5
18	1.9705521561584637E-4	1.751601916585301E-5	3.9411043123169274E-5	2.6274028748779517E-5	7.882208624633855E-5
19	2.1895023957316264E-4	1.751601916585301E-5	3.9411043123169274E-5	2.6274028748779517E-5	8.758009582926505E-5
20	2.4522426832194213E-4	1.751601916585301E-5	3.9411043123169274E-5	3.065303354024277E-5	9.195910062072831E-5
21	2.8463531144511144E-4	1.751601916585301E-5	3.9411043123169274E-5	3.065303354024277E-5	9.195910062072831E-5
22	3.28425359359744E-4	1.751601916585301E-5	3.9411043123169274E-5	3.065303354024277E-5	9.633810541219156E-5
23	3.590783928999867E-4	1.751601916585301E-5	3.9411043123169274E-5	3.503203833170602E-5	1.0947511978658132E-4
24	4.0286844081461926E-4	1.751601916585301E-5	3.9411043123169274E-5	3.503203833170602E-5	1.1823312936950783E-4
25	4.597955031036415E-4	1.751601916585301E-5	4.379004791463253E-5	3.9411043123169274E-5	1.2261213416097107E-4
26	5.429965941414433E-4	1.751601916585301E-5	4.379004791463253E-5	4.816905270609578E-5	1.2261213416097107E-4
27	5.955446516390023E-4	1.751601916585301E-5	4.379004791463253E-5	4.816905270609578E-5	1.3137014374389757E-4
28	6.74366737885341E-4	1.751601916585301E-5	4.379004791463253E-5	4.816905270609578E-5	1.3574914853536083E-4
29	7.882208624633855E-4	1.751601916585301E-5	4.816905270609578E-5	5.2548057497559034E-5	1.3574914853536083E-4
30	8.84558967875577E-4	1.751601916585301E-5	4.816905270609578E-5	5.2548057497559034E-5	1.4450715811828733E-4
31	9.765180684963054E-4	1.751601916585301E-5	4.816905270609578E-5	5.2548057497559034E-5	1.4450715811828733E-4
32	0.0011210252266145926	1.751601916585301E-5	4.816905270609578E-5	5.2548057497559034E-5	1.488861629097506E-4
33	0.0013574914853536084	2.1895023957316263E-5	5.2548057497559034E-5	6.130606708048553E-5	1.576441724926771E-4
34	0.0014932406338889692	2.1895023957316263E-5	5.2548057497559034E-5	6.130606708048553E-5	1.576441724926771E-4
35	0.001593957744092624	2.6274028748779517E-5	5.2548057497559034E-5	6.568507187194879E-5	1.751601916585301E-4
36	0.001699053859087742	2.6274028748779517E-5	5.2548057497559034E-5	6.568507187194879E-5	1.8829720603291987E-4
37	0.0018610770363718823	2.6274028748779517E-5	5.2548057497559034E-5	6.568507187194879E-5	1.8829720603291987E-4
38	0.0019793101657413904	2.6274028748779517E-5	5.2548057497559034E-5	7.006407666341204E-5	2.0143422040730963E-4
39	0.0021719863765657732	3.065303354024277E-5	5.2548057497559034E-5	7.006407666341204E-5	2.0143422040730963E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	177510	0.0	43.249535	12
CAACGCA	178245	0.0	43.168396	13
GTATCAA	178495	0.0	42.94784	9
AACGCAG	179255	0.0	42.908844	14
ATCAACG	178920	0.0	42.881035	11
GGTATCA	178810	0.0	42.878475	8
ACGCAGA	179455	0.0	42.863533	15
GTGGTAT	179195	0.0	42.817738	6
AGTGGTA	179805	0.0	42.68374	5
TGGTATC	180070	0.0	42.60593	7
TATCAAC	180120	0.0	42.561626	10
CGCAGAG	179940	0.0	42.47916	16
AAGCAGT	181580	0.0	42.369343	1
AGAGTAC	178435	0.0	42.321712	19
CAGAGTA	181115	0.0	41.843304	18
GCAGAGT	183885	0.0	41.46382	17
CAGTGGT	185580	0.0	41.445198	4
GTACATG	114195	0.0	41.28793	22
AGCAGTG	186655	0.0	41.195656	2
GCAGTGG	187185	0.0	41.16676	3
>>END_MODULE