FastQCFastQC Report
Thu 26 May 2016
SRR805303_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805303_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20340675
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT422250.2075889811916271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC224300.032.54123712
CAACGCA226700.032.23643513
AACGCAG234850.031.15605514
ATCAACG237500.030.77052311
ACGCAGA238350.030.75519215
GTATCAA240200.030.4808489
GGTATCA240150.030.4590858
AGTGGTA242700.030.3708255
GTGGTAT242300.030.2816736
AAGCAGT242600.030.0123651
TGGTATC251450.029.1618587
TATCAAC252550.029.08829910
CGCAGAG258200.028.12064216
AGAGTAC260500.027.61324119
CAGTGGT288700.025.749914
CAGAGTA286500.025.35863718
GCAGAGT300150.024.31783917
GCAGTGG304500.024.3103433
AGCAGTG301200.024.2928282
GTACATG200050.023.61909322
Produced by FastQC (version 0.11.5)