##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805303_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20340675
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.103367071151766	37.0	37.0	37.0	35.0	37.0
2	36.04740437571516	37.0	37.0	37.0	35.0	37.0
3	37.836274509080944	39.0	38.0	39.0	35.0	39.0
4	37.86512192933617	39.0	38.0	39.0	35.0	39.0
5	37.8795379209392	39.0	38.0	39.0	35.0	39.0
6	37.872114519306756	39.0	38.0	39.0	35.0	39.0
7	37.81246949769366	39.0	38.0	39.0	35.0	39.0
8	38.67376530031574	40.0	39.0	40.0	36.0	40.0
9	38.6356811167771	40.0	39.0	40.0	36.0	40.0
10	38.61421939045779	40.0	39.0	40.0	36.0	40.0
11	38.54122200959408	40.0	38.0	40.0	36.0	40.0
12	38.36354029549167	40.0	38.0	40.0	35.0	40.0
13	38.47135574409404	40.0	38.0	40.0	35.0	40.0
14	38.37836064929015	40.0	38.0	40.0	35.0	40.0
15	38.32619222321777	40.0	38.0	40.0	35.0	40.0
16	38.28250714393696	40.0	38.0	40.0	35.0	40.0
17	38.19014482066107	40.0	38.0	40.0	34.0	40.0
18	38.07603931531279	40.0	38.0	40.0	34.0	40.0
19	37.97842977187335	40.0	38.0	40.0	34.0	40.0
20	37.79732855473085	40.0	38.0	40.0	34.0	40.0
21	37.68419219126209	40.0	37.0	40.0	34.0	40.0
22	36.87770971218998	39.0	37.0	40.0	32.0	40.0
23	37.125216247740056	39.0	37.0	40.0	32.0	40.0
24	37.05102519950788	39.0	36.0	40.0	32.0	40.0
25	36.982203638768134	38.0	36.0	40.0	32.0	40.0
26	36.83937922414079	38.0	36.0	40.0	32.0	40.0
27	36.69584200131018	38.0	36.0	40.0	31.0	40.0
28	36.54072251781221	38.0	36.0	40.0	31.0	40.0
29	36.43412866092202	38.0	36.0	40.0	31.0	40.0
30	36.153718005916716	38.0	35.0	40.0	30.0	40.0
31	35.71616817042699	38.0	35.0	39.0	30.0	40.0
32	35.66946681956228	38.0	34.0	39.0	30.0	40.0
33	35.56573963253432	38.0	34.0	39.0	30.0	40.0
34	35.369649778092416	37.0	34.0	39.0	30.0	40.0
35	35.21652034654701	37.0	34.0	39.0	29.0	40.0
36	34.9369220539633	37.0	33.0	39.0	29.0	40.0
37	34.70942419560806	37.0	33.0	38.0	28.0	40.0
38	34.46307656948454	36.0	33.0	38.0	27.0	40.0
39	34.205993163943674	36.0	33.0	38.0	27.0	40.0
40	33.918357429141366	36.0	32.0	38.0	27.0	39.0
41	33.81119898921742	36.0	32.0	38.0	26.0	39.0
42	33.65621352290423	35.0	32.0	38.0	26.0	39.0
43	33.55454467464821	35.0	31.0	38.0	26.0	39.0
44	33.27567679047033	35.0	31.0	38.0	25.0	39.0
45	32.98791893582686	35.0	31.0	38.0	24.0	39.0
46	32.74742460611558	34.0	31.0	38.0	24.0	39.0
47	32.44544037992839	34.0	30.0	37.0	24.0	39.0
48	32.11568514810841	34.0	30.0	37.0	23.0	39.0
49	31.64622973426398	34.0	29.0	37.0	23.0	38.0
50	31.45888054354145	33.0	29.0	37.0	22.0	38.0
51	32.56817303260585	34.0	31.0	37.0	25.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	35.0
8	156.0
9	394.0
10	564.0
11	611.0
12	854.0
13	1089.0
14	1416.0
15	2065.0
16	3265.0
17	5304.0
18	9069.0
19	14713.0
20	23800.0
21	36413.0
22	53314.0
23	73354.0
24	98115.0
25	125648.0
26	157357.0
27	193547.0
28	235690.0
29	282882.0
30	339939.0
31	412697.0
32	511857.0
33	665842.0
34	945757.0
35	1555952.0
36	3339895.0
37	7013455.0
38	4111013.0
39	124610.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.258824989829492	28.46926171329123	21.735232483681095	26.53668081319819
2	25.629916411328534	23.228531009909947	28.430251208477596	22.711301370283927
3	21.57095081652895	25.00977474936304	26.801475368934412	26.617799065173596
4	22.515767052961618	27.64505602690176	25.993866968524888	23.845309951611735
5	25.84065179744527	27.161645323962947	24.570049912306253	22.42765296628553
6	24.97437277769789	25.28713525976891	26.478064272694983	23.260427689838217
7	24.28597871014605	26.140504186807963	26.502901206572542	23.070615896473445
8	23.72833251600549	25.18093426103116	26.802124314950216	24.28860890801313
9	23.723195026713714	25.05533862568474	27.250511598066435	23.970954749535107
10	23.44987076387583	25.71340921577086	27.155755647243762	23.680964373109546
11	23.34814847589866	26.54479755465342	26.433665549447106	23.67338842000081
12	23.49376802883877	25.474361101585863	27.056299754064213	23.975571115511162
13	23.668118191751255	25.801390563489168	25.945835130840056	24.58465611391952
14	23.673796469389536	26.512659978098075	26.015341182138744	23.798202370373648
15	23.656024197820376	26.35545280576972	25.922202680097882	24.06632031631202
16	23.979764683325406	25.67024447320455	25.740301145365134	24.609689698104905
17	24.780539485538213	25.590763335041732	25.77701084157728	23.85168633784277
18	23.870112471685427	25.48559966667773	25.73377235514554	24.910515506491304
19	24.092587881178968	26.25610015400177	25.67847920484448	23.97283275997478
20	24.7077936204182	25.5625243508389	25.797408394755827	23.932273633987073
21	23.991204814982787	26.134432608554043	25.770786859334805	24.103575717128365
22	24.898136369614086	25.330388494973743	25.730483378747266	24.04099175666491
23	23.922052734238168	25.533484016631704	26.675196373768323	23.869266875361806
24	23.886203383122734	26.14682157794665	25.77668636856938	24.190288670361234
25	24.003170986213583	25.55439777686827	25.660190726217298	24.78224051070085
26	24.008121657712934	25.966237600276294	26.151408446376536	23.87423229563424
27	23.986947335818503	25.33170605203613	26.546867299143216	24.13447931300215
28	24.62559870800748	25.497128290973624	25.869264417232955	24.008008583785937
29	24.590098411188418	25.49786572962795	25.99560240749139	23.91643345169224
30	24.524191060522817	25.333505402352674	25.970755641098442	24.171547896026066
31	24.542686021973214	25.417416088699124	25.816812863879885	24.22308502544778
32	24.32601179656034	25.673862838868427	25.933254427397323	24.066870937173913
33	24.24305486420682	25.47998530038949	25.943072194015194	24.3338876413885
34	24.31936993241375	25.637531694498833	25.823395732934134	24.21970264015329
35	24.262847717688818	25.658234055654493	25.94487154433174	24.134046682324946
36	24.18616884641242	25.490456929280864	25.93673022158802	24.38664400271869
37	24.377740660032178	25.631946825756767	25.742331559793367	24.247980954417688
38	24.333730321142244	25.665844422567098	25.913009278207337	24.087415978083325
39	24.259794721659926	25.484867144281104	25.91707993957919	24.33825819447978
40	24.36306563081117	25.634321378223685	25.739553874195426	24.26305911676972
41	24.254082030217777	25.649581442110453	25.969693729436216	24.126642798235558
42	24.247926875583037	25.49801813361651	25.964693895359915	24.28936109544054
43	24.38726345118832	25.593604931989724	25.730896344393685	24.288235272428274
44	24.31450775355292	25.553709500790905	25.972014203068483	24.1597685425877
45	24.290157529187205	25.36000403133131	25.965396920210367	24.384441519271117
46	24.398211957076153	25.56282915881602	25.748471965655025	24.290486918452807
47	24.270059867728087	25.566776913745485	25.95489579377282	24.208267424753604
48	24.253369172851933	25.409323928532363	25.919326669346027	24.417980229269677
49	24.512967244204038	25.562175296542517	25.73220898519838	24.192648474055066
50	24.318008129032098	25.59800006636948	25.91173105120651	24.17226075339191
51	24.314837142818515	25.43768090291989	25.868856367844234	24.378625586417364
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3512.0
1	2562.0
2	1612.0
3	1490.0
4	1368.0
5	1511.5
6	1655.0
7	1919.0
8	2183.0
9	2708.5
10	3234.0
11	4563.0
12	5892.0
13	8270.0
14	10648.0
15	14227.0
16	17806.0
17	24772.0
18	31738.0
19	42949.0
20	54160.0
21	70480.5
22	86801.0
23	132413.5
24	178026.0
25	183591.5
26	217537.5
27	245918.0
28	280122.0
29	314326.0
30	351728.5
31	389131.0
32	434669.0
33	480207.0
34	537474.5
35	594742.0
36	666499.5
37	738257.0
38	807584.5
39	876912.0
40	956506.5
41	1036101.0
42	1110992.5
43	1185884.0
44	1262928.5
45	1339973.0
46	1405456.5
47	1470940.0
48	1509757.0
49	1548574.0
50	1574765.0
51	1600956.0
52	1595913.0
53	1590870.0
54	1533573.0
55	1476276.0
56	1384627.0
57	1292978.0
58	1190451.5
59	1087925.0
60	968104.5
61	848284.0
62	732149.5
63	616015.0
64	519738.5
65	423462.0
66	342089.5
67	260717.0
68	213325.0
69	165933.0
70	125448.0
71	84963.0
72	63157.5
73	41352.0
74	31122.0
75	15867.0
76	10842.0
77	7991.0
78	5140.0
79	4001.5
80	2863.0
81	2239.5
82	1616.0
83	1095.0
84	574.0
85	374.0
86	174.0
87	112.5
88	51.0
89	36.0
90	21.0
91	15.0
92	9.0
93	6.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.0340675E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.31760483193618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.31507145018033	38.87986541187637
2	13.364578916801124	13.984055170288537
3	4.821919781168542	7.568138809274158
4	2.2147145831832153	4.634742495140228
5	1.2698387212901434	3.321746021037444
6	0.8139679317375124	2.5550911559106946
7	0.5488510724790846	2.010020146508158
8	0.38378600366677	1.6063011583892868
9	0.30627576132450063	1.4421252825518065
>10	1.8360757557846508	17.944426508277072
>50	0.10106006192174705	3.4892109847454065
>100	0.02335179151250476	2.1263819650091897
>500	4.302143181180853E-4	0.13288767881241198
>1k	6.821573860863253E-5	0.08986426554023695
>5k	0.0	0.0
>10k+	9.738893151188092E-6	0.21514294663902414
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	42225	0.2075889811916271	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8514294633781817E-4	0.0	0.0	0.0	1.4748773086438872E-5
2	2.900592040332978E-4	0.0	0.0	0.0	1.4748773086438872E-5
3	2.9497546172877745E-4	0.0	0.0	1.4748773086438872E-5	2.9497546172877743E-5
4	2.9497546172877745E-4	0.0	0.0	1.4748773086438872E-5	4.424631925931662E-5
5	2.9497546172877745E-4	0.0	0.0	1.4748773086438872E-5	6.882760773671474E-5
6	2.998917194242571E-4	0.0	0.0	1.4748773086438872E-5	8.849263851863324E-5
7	2.998917194242571E-4	0.0	0.0	2.4581288477398118E-5	1.032414116050721E-4
8	2.998917194242571E-4	4.916257695479624E-6	0.0	3.933006156383699E-5	1.032414116050721E-4
9	3.0480797711973667E-4	4.916257695479624E-6	0.0	3.933006156383699E-5	1.0815766930055172E-4
10	3.0480797711973667E-4	9.832515390959248E-6	0.0	3.933006156383699E-5	1.229064423869906E-4
11	3.0480797711973667E-4	9.832515390959248E-6	0.0	4.424631925931662E-5	1.2782270008247022E-4
12	3.0480797711973667E-4	9.832515390959248E-6	0.0	4.9162576954796236E-5	1.4257147316890908E-4
13	3.1464049251069595E-4	9.832515390959248E-6	0.0	5.407883465027586E-5	1.6223650395082758E-4
14	3.1464049251069595E-4	9.832515390959248E-6	0.0	5.407883465027586E-5	1.7206901934178683E-4
15	3.1464049251069595E-4	9.832515390959248E-6	0.0	5.407883465027586E-5	1.7698527703726647E-4
16	3.1464049251069595E-4	9.832515390959248E-6	0.0	6.391135004123511E-5	2.064828232101442E-4
17	3.3430552329261445E-4	9.832515390959248E-6	0.0	9.340889621411286E-5	2.1139908090562383E-4
18	3.3922178098809403E-4	1.4748773086438872E-5	0.0	1.032414116050721E-4	2.261478539920627E-4
19	3.5888681177001253E-4	1.4748773086438872E-5	0.0	1.032414116050721E-4	2.4089662707850158E-4
20	4.129656464202884E-4	1.4748773086438872E-5	0.0	1.032414116050721E-4	2.4089662707850158E-4
21	4.4246319259316617E-4	1.4748773086438872E-5	0.0	1.1307392699603135E-4	2.5564540016494045E-4
22	4.7687699646152353E-4	2.4581288477398118E-5	0.0	1.1307392699603135E-4	2.605616578604201E-4
23	5.35872088807279E-4	3.933006156383699E-5	0.0	1.1307392699603135E-4	2.6547791555589967E-4
24	5.997834388485142E-4	4.424631925931662E-5	0.0	1.1307392699603135E-4	2.7531043094685895E-4
25	6.5386227349879E-4	4.9162576954796236E-5	0.0	1.1307392699603135E-4	2.802266886423386E-4
26	7.226898812355047E-4	4.9162576954796236E-5	0.0	1.1307392699603135E-4	2.900592040332978E-4
27	7.571036851038621E-4	4.9162576954796236E-5	0.0	1.1799018469151097E-4	3.0480797711973667E-4
28	8.50512581317975E-4	5.407883465027586E-5	0.0	1.1799018469151097E-4	3.1955675020617553E-4
29	9.488377352275674E-4	5.407883465027586E-5	0.0	1.1799018469151097E-4	3.4413803868357367E-4
30	0.0010176653429642823	5.407883465027586E-5	0.0	1.229064423869906E-4	3.5397055407453295E-4
31	0.001150404300742232	5.407883465027586E-5	0.0	1.229064423869906E-4	3.5888681177001253E-4
32	0.0012880595162156614	5.407883465027586E-5	0.0	1.229064423869906E-4	3.9821687333384953E-4
33	0.0014994585971212853	5.407883465027586E-5	0.0	1.229064423869906E-4	4.080493887248088E-4
34	0.0016813601318540314	5.407883465027586E-5	0.0	1.229064423869906E-4	4.277144195067273E-4
35	0.0017993503165455423	5.407883465027586E-5	0.0	1.229064423869906E-4	4.4246319259316617E-4
36	0.0019960006243647274	5.407883465027586E-5	0.0	1.229064423869906E-4	4.522957079841254E-4
37	0.0021631533860110345	6.391135004123511E-5	0.0	1.229064423869906E-4	4.6704448107056425E-4
38	0.0023843849823076174	6.882760773671474E-5	0.0	1.2782270008247022E-4	4.8179325415700317E-4
39	0.002703941732513793	6.882760773671474E-5	0.0	1.3765521547342947E-4	4.96542027243442E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	22430	0.0	32.541237	12
CAACGCA	22670	0.0	32.236435	13
AACGCAG	23485	0.0	31.156055	14
ATCAACG	23750	0.0	30.770523	11
ACGCAGA	23835	0.0	30.755192	15
GTATCAA	24020	0.0	30.480848	9
GGTATCA	24015	0.0	30.459085	8
AGTGGTA	24270	0.0	30.370825	5
GTGGTAT	24230	0.0	30.281673	6
AAGCAGT	24260	0.0	30.012365	1
TGGTATC	25145	0.0	29.161858	7
TATCAAC	25255	0.0	29.088299	10
CGCAGAG	25820	0.0	28.120642	16
AGAGTAC	26050	0.0	27.613241	19
CAGTGGT	28870	0.0	25.74991	4
CAGAGTA	28650	0.0	25.358637	18
GCAGAGT	30015	0.0	24.317839	17
GCAGTGG	30450	0.0	24.310343	3
AGCAGTG	30120	0.0	24.292828	2
GTACATG	20005	0.0	23.619093	22
>>END_MODULE