FastQCFastQC Report
Thu 26 May 2016
SRR805302_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805302_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18239639
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2322551.2733530526563601No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT754810.4138294623045993No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG683360.37465653788432984No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC255530.14009597448721436No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTCA203160.11138378341808189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1973900.043.4338112
CAACGCA1974550.043.41039713
GTATCAA1976800.043.3006639
AACGCAG1984400.043.2153314
AGTGGTA1981200.043.209045
GGTATCA1983050.043.1925548
ACGCAGA1988200.043.1587615
AAGCAGT1992350.043.156951
ATCAACG1984600.043.1520211
GTGGTAT1988100.043.1496166
TGGTATC1991450.043.0566947
TATCAAC1992600.042.95279710
AGAGTAC1931800.042.90467519
CGCAGAG2001850.042.87009416
ACATGGG1604550.042.46050324
CAGAGTA1963400.042.3768718
GTACATG1623900.042.36744722
GAGTACA1643300.042.31637220
AGTACAT1631400.042.31472821
CAGTGGT2028600.042.2959184