##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805302_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18239639
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.50641994614038	37.0	37.0	37.0	35.0	37.0
2	36.487829172496234	37.0	37.0	37.0	35.0	37.0
3	38.293604933737996	39.0	39.0	39.0	37.0	39.0
4	38.251499495138034	39.0	39.0	39.0	37.0	39.0
5	38.21792240515286	39.0	39.0	39.0	37.0	39.0
6	38.22082630034509	39.0	39.0	39.0	37.0	39.0
7	38.1211879248268	39.0	39.0	39.0	37.0	39.0
8	39.144002301799944	40.0	40.0	40.0	38.0	40.0
9	39.15615522873013	40.0	40.0	40.0	38.0	40.0
10	38.90701789657131	40.0	39.0	40.0	37.0	40.0
11	39.04106863079911	40.0	39.0	40.0	37.0	40.0
12	39.089326164843506	40.0	40.0	40.0	37.0	40.0
13	39.14421392879541	40.0	40.0	40.0	37.0	40.0
14	39.11979771090864	40.0	40.0	40.0	37.0	40.0
15	39.08960944895894	40.0	40.0	40.0	37.0	40.0
16	39.03601973701343	40.0	40.0	40.0	37.0	40.0
17	39.00362112429966	40.0	40.0	40.0	37.0	40.0
18	38.97186567124492	40.0	40.0	40.0	37.0	40.0
19	38.943647897855875	40.0	40.0	40.0	37.0	40.0
20	38.86149950665142	40.0	40.0	40.0	37.0	40.0
21	38.76097015955195	40.0	39.0	40.0	36.0	40.0
22	38.793959244478465	40.0	39.0	40.0	36.0	40.0
23	38.411588683306725	40.0	38.0	40.0	35.0	40.0
24	38.17585030054597	40.0	38.0	40.0	35.0	40.0
25	38.5494855462874	40.0	39.0	40.0	36.0	40.0
26	38.60972242926518	40.0	39.0	40.0	36.0	40.0
27	38.64729652818238	40.0	39.0	40.0	36.0	40.0
28	38.659501923256265	40.0	39.0	40.0	36.0	40.0
29	38.693612302304885	40.0	39.0	40.0	36.0	40.0
30	38.69493486137527	40.0	39.0	40.0	36.0	40.0
31	38.63467785738523	40.0	39.0	40.0	36.0	40.0
32	38.62029703548409	40.0	39.0	40.0	36.0	40.0
33	38.506966393358994	40.0	39.0	40.0	35.0	40.0
34	38.491899812271505	40.0	39.0	40.0	35.0	40.0
35	38.45262047127139	40.0	39.0	40.0	35.0	40.0
36	38.389727559849185	40.0	39.0	40.0	35.0	40.0
37	38.32706151695217	40.0	38.0	40.0	35.0	40.0
38	38.2392377941252	40.0	38.0	40.0	35.0	40.0
39	38.19261751836207	40.0	38.0	40.0	35.0	40.0
40	38.12839793594599	40.0	38.0	40.0	35.0	40.0
41	38.073357098789074	40.0	38.0	40.0	35.0	40.0
42	38.0174764972048	40.0	38.0	40.0	34.0	40.0
43	37.972348520713595	40.0	38.0	40.0	34.0	40.0
44	37.89578127067098	40.0	38.0	40.0	34.0	40.0
45	37.73001494163344	40.0	37.0	40.0	34.0	40.0
46	37.677656065451735	40.0	37.0	40.0	34.0	40.0
47	37.61936297094476	40.0	37.0	40.0	34.0	40.0
48	37.52158954461763	40.0	37.0	40.0	33.0	40.0
49	37.39678071479375	40.0	36.0	40.0	33.0	40.0
50	37.27933129597576	40.0	36.0	40.0	33.0	40.0
51	36.614027064899695	39.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	6.0
8	31.0
9	159.0
10	191.0
11	216.0
12	224.0
13	241.0
14	298.0
15	403.0
16	644.0
17	1073.0
18	1938.0
19	3339.0
20	5889.0
21	9836.0
22	14729.0
23	20745.0
24	29109.0
25	38265.0
26	50190.0
27	63907.0
28	81000.0
29	99884.0
30	124593.0
31	154180.0
32	193116.0
33	244578.0
34	324269.0
35	460641.0
36	711029.0
37	1464381.0
38	2800529.0
39	1.1340005E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.783715785164388	35.70153992631104	28.535329016106076	21.979415272418496
2	28.04871850808012	41.06571955727852	12.254908115231885	18.630653819409474
3	36.166910978885056	24.518676054937274	17.363095837587576	21.9513171285901
4	22.839530979752396	22.574114542508212	18.690101267903383	35.896253209836004
5	21.51753661352618	33.20203870263003	22.299964379777474	22.980460304066323
6	33.13268974237922	22.39170961662125	21.001314773828582	23.474285867170945
7	23.06240819788155	21.774350906835384	32.43578998465924	22.727450910623833
8	32.345333150508075	23.712678743258024	22.185334918086923	21.75665318814698
9	32.497918407266724	23.20183529948153	22.241109048265702	22.059137244986044
10	21.791856735761055	23.43921390110846	32.8386817304882	21.930247632642292
11	21.776774200410436	33.65421322209283	22.573895239922237	21.9951173375745
12	21.564319337679873	23.095265207825662	33.155508176450205	22.184907278044264
13	21.684371055808725	23.139926179460023	22.91139643717729	32.26430632755396
14	22.095453753223953	33.65266713886169	22.474523755651084	21.777355352263278
15	21.813567691772846	33.448847315453996	22.557146004918188	22.180438987854966
16	22.025096001077653	22.78537968870985	22.818275076606508	32.371249233605994
17	32.45784634224395	22.80365855925109	22.65339242733916	22.085102671165803
18	21.967907369219315	22.849169328406116	22.68360135855759	32.49932194381698
19	22.009925744692644	33.249079107322245	22.750378995987806	21.990616151997305
20	32.64930846493179	22.963656243415784	22.629603579325227	21.757431712327204
21	22.129286659675664	33.13701000332298	22.627503757064492	22.10619957993686
22	32.25909789113699	22.71663929313513	23.014496065410068	22.009766750317812
23	22.199183876391412	22.873572223660787	32.97169971401298	21.95554418593482
24	22.276795061568926	32.486629806653525	22.641456884097323	22.595118247680233
25	21.867724465380046	22.973349417715998	22.777994674127047	32.38093144277691
26	22.238828301371534	31.520497746693344	24.225682317506394	22.01499163442873
27	22.343638489774932	22.844448840242944	32.77954130561466	22.032371364367464
28	30.773421557301656	22.792512505318776	24.3461616756779	22.08790426170167
29	31.043048604196606	22.923178468608945	24.01085898684727	22.022913940347173
30	30.866537435307794	22.84566596959512	24.102648084208244	22.185148510888837
31	28.01400290871985	23.975178456108697	24.932154633104307	23.078664002067146
32	26.307253120525026	25.117229567975553	25.078785824653654	23.496731486845764
33	25.81538483300026	24.399841466160595	25.723392880747255	24.061380820091888
34	24.7890432480599	24.261472499537955	26.217366473097414	24.732117779304733
35	24.52953701550782	24.43316997666456	26.554412617486562	24.48288039034106
36	24.731525662322593	24.500802894180087	26.6140629208725	24.15360852262482
37	25.45053112070913	24.31131449476604	26.25071691385997	23.98743747066485
38	25.50369006755013	24.550343348352456	26.18223968138843	23.763726902708985
39	25.272210705486003	24.503955368853518	26.308766308368277	23.91506761729221
40	24.780605581064403	25.107585736757184	25.946198825535966	24.165609856642448
41	25.219062723774304	25.410875730599713	25.602436539451247	23.76762500617474
42	25.0527655728274	25.361784846728604	25.913774938199165	23.671674642244838
43	24.725198782717136	25.033856207351473	26.613821688027926	23.627123321903465
44	25.269770964217	24.596205001645043	26.15728304710417	23.97674098703379
45	25.572622352887574	24.0873517288363	26.111015684027517	24.229010234248605
46	25.02453584744742	24.20917979791157	25.847885476242155	24.918398878398854
47	24.718904798499576	24.391869817160305	26.08540662454997	24.803818759790147
48	24.39427666304141	24.373667702524155	26.25173118832012	24.980324446114313
49	24.14923343603456	25.133896564509854	25.93919759047863	24.777672408976954
50	25.185147578852852	25.014727539289566	25.56871328429252	24.23141159756506
51	24.871024037263016	24.77784785104574	26.11097730607497	24.240150805616274
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	372.0
1	236.5
2	101.0
3	104.5
4	108.0
5	130.0
6	152.0
7	230.5
8	309.0
9	416.5
10	524.0
11	883.0
12	1242.0
13	1894.0
14	2546.0
15	3699.0
16	4852.0
17	7691.0
18	10530.0
19	15341.5
20	20153.0
21	29121.0
22	38089.0
23	172180.0
24	306271.0
25	217021.5
26	140201.5
27	152631.0
28	178857.0
29	205083.0
30	232677.5
31	260272.0
32	295655.0
33	331038.0
34	378170.0
35	425302.0
36	493164.5
37	561027.0
38	626092.0
39	691157.0
40	764911.5
41	838666.0
42	911167.5
43	983669.0
44	1066511.0
45	1149353.0
46	1229837.0
47	1310321.0
48	1359786.5
49	1409252.0
50	1454686.0
51	1500120.0
52	1521281.5
53	1542443.0
54	1545974.5
55	1549506.0
56	1465576.5
57	1381647.0
58	1226796.5
59	1071946.0
60	941362.5
61	810779.0
62	703935.0
63	597091.0
64	502950.0
65	408809.0
66	324960.5
67	241112.0
68	194874.0
69	148636.0
70	112379.5
71	76123.0
72	57629.0
73	39135.0
74	30203.5
75	15887.0
76	10502.0
77	7869.0
78	5236.0
79	3825.5
80	2415.0
81	1807.0
82	1199.0
83	850.5
84	502.0
85	354.5
86	207.0
87	142.0
88	77.0
89	60.5
90	44.0
91	31.0
92	18.0
93	19.0
94	20.0
95	12.0
96	4.0
97	4.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.8239639E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.065777104883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.77397682371742	26.867088492117336
2	15.314656802617094	11.96557938177639
3	5.543061291987572	6.496319907344737
4	2.681462127602483	4.190136071684157
5	1.571625482843924	3.0698385402567427
6	1.0089999283300006	2.3650419779389633
7	0.7101881562687252	1.9420836550725284
8	0.5353187272612435	1.6730113663405912
9	0.4183831084037376	1.471001513161366
>10	3.1025589202465835	23.462436072534086
>50	0.2447865031704864	6.518337897916325
>100	0.09177224011186676	5.9843642451601555
>500	0.0021642561469096457	0.5583556717036251
>1k	8.278506638194466E-4	0.6317622789335023
>5k	1.3066837695898049E-4	0.3344517620127892
>10k+	8.711225130598686E-5	2.4701911660468694
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	232255	1.2733530526563601	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT	75481	0.4138294623045993	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG	68336	0.37465653788432984	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC	25553	0.14009597448721436	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTCA	20316	0.11138378341808189	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4671540922493036E-4	0.0	0.0	0.0	2.7412823247214486E-5
2	2.7961079712158777E-4	0.0	0.0	0.0	3.837795254610028E-5
3	2.9057592642047354E-4	0.0	0.0	0.0	6.579077579331477E-5
4	3.0154105571935936E-4	0.0	0.0	0.0	6.579077579331477E-5
5	3.0154105571935936E-4	0.0	0.0	0.0	9.320359904052925E-5
6	3.344364436160167E-4	0.0	0.0	0.0	1.0416872833941505E-4
7	3.344364436160167E-4	0.0	0.0	0.0	1.2061642228774374E-4
8	3.344364436160167E-4	0.0	0.0	0.0	1.3158155158662953E-4
9	3.4540157291490254E-4	0.0	0.0	0.0	1.6447693948328692E-4
10	3.4540157291490254E-4	0.0	0.0	0.0	2.083374566788301E-4
11	3.4540157291490254E-4	0.0	0.0	0.0	2.521979738743733E-4
12	3.4540157291490254E-4	5.482564649442897E-6	0.0	0.0	2.576805385238162E-4
13	3.8377952546100283E-4	5.482564649442897E-6	0.0	0.0	2.7961079712158777E-4
14	4.166749133576602E-4	5.482564649442897E-6	0.0	0.0	3.0154105571935936E-4
15	4.27640042656546E-4	5.482564649442897E-6	0.0	5.482564649442897E-6	3.0702362036880225E-4
16	4.550528659037605E-4	5.482564649442897E-6	0.0	1.0965129298885794E-5	3.1798874966768807E-4
17	5.153610770476324E-4	5.482564649442897E-6	0.0	1.0965129298885794E-5	3.2895387896657383E-4
18	6.140472407376045E-4	5.482564649442897E-6	0.0	1.0965129298885794E-5	3.344364436160167E-4
19	7.072508397781337E-4	5.482564649442897E-6	0.0	1.0965129298885794E-5	3.4540157291490254E-4
20	8.05937003468106E-4	5.482564649442897E-6	0.0	1.0965129298885794E-5	3.892620901104457E-4
21	8.772103439108636E-4	5.482564649442897E-6	0.0	1.0965129298885794E-5	4.057097840587744E-4
22	9.923442015491643E-4	5.482564649442897E-6	0.0	1.0965129298885794E-5	4.166749133576602E-4
23	0.0011348908824346797	5.482564649442897E-6	0.0	1.0965129298885794E-5	4.331226073059889E-4
24	0.0013487109037629529	5.482564649442897E-6	0.0	1.0965129298885794E-5	4.550528659037605E-4
25	0.0015789786190395544	5.482564649442897E-6	0.0	1.0965129298885794E-5	4.550528659037605E-4
26	0.001814728898965599	5.482564649442897E-6	0.0	1.0965129298885794E-5	4.8246568915097496E-4
27	0.002028548920293872	5.482564649442897E-6	0.0	1.0965129298885794E-5	5.263262063465181E-4
28	0.0022752643295188024	5.482564649442897E-6	0.0	1.0965129298885794E-5	5.372913356454039E-4
29	0.0025329448680426188	5.482564649442897E-6	0.0	1.0965129298885794E-5	5.537390295937327E-4
30	0.003059271074389137	5.482564649442897E-6	0.0	1.0965129298885794E-5	5.756692881915042E-4
31	0.0036952485737245127	5.482564649442897E-6	0.0	1.0965129298885794E-5	5.811518528409471E-4
32	0.004336708637709332	5.482564649442897E-6	0.0	1.0965129298885794E-5	5.866344174903901E-4
33	0.0047917615036130925	5.482564649442897E-6	0.0	1.0965129298885794E-5	6.030821114387187E-4
34	0.005296157451361839	5.482564649442897E-6	0.0	1.0965129298885794E-5	6.030821114387187E-4
35	0.0057512103172656	5.482564649442897E-6	0.0	1.0965129298885794E-5	6.195298053870474E-4
36	0.006266571394313232	5.482564649442897E-6	0.0	1.0965129298885794E-5	6.41460063984819E-4
37	0.006787415036010307	5.482564649442897E-6	0.0	1.0965129298885794E-5	6.688728872320334E-4
38	0.0074343576646445685	5.482564649442897E-6	0.0	1.0965129298885794E-5	6.743554518814764E-4
39	0.008103230551876603	5.482564649442897E-6	0.0	1.0965129298885794E-5	6.798380165309193E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	197390	0.0	43.43381	12
CAACGCA	197455	0.0	43.410397	13
GTATCAA	197680	0.0	43.300663	9
AACGCAG	198440	0.0	43.21533	14
AGTGGTA	198120	0.0	43.20904	5
GGTATCA	198305	0.0	43.192554	8
ACGCAGA	198820	0.0	43.15876	15
AAGCAGT	199235	0.0	43.15695	1
ATCAACG	198460	0.0	43.15202	11
GTGGTAT	198810	0.0	43.149616	6
TGGTATC	199145	0.0	43.056694	7
TATCAAC	199260	0.0	42.952797	10
AGAGTAC	193180	0.0	42.904675	19
CGCAGAG	200185	0.0	42.870094	16
ACATGGG	160455	0.0	42.460503	24
CAGAGTA	196340	0.0	42.37687	18
GTACATG	162390	0.0	42.367447	22
GAGTACA	164330	0.0	42.316372	20
AGTACAT	163140	0.0	42.314728	21
CAGTGGT	202860	0.0	42.295918	4
>>END_MODULE