FastQCFastQC Report
Thu 26 May 2016
SRR805301_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805301_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11278606
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT251400.2228998867413225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC139600.035.07163212
CAACGCA139700.035.03042213
AACGCAG144100.033.9920214
ACGCAGA144300.033.9449115
GTATCAA148700.032.8648269
GGTATCA149000.032.828868
ATCAACG150450.032.55732711
AGTGGTA152850.032.0019655
GTGGTAT153000.031.9411776
CGCAGAG152600.031.70052116
TGGTATC154900.031.6074897
AAGCAGT155600.031.1616331
TATCAAC157900.031.0069710
AGAGTAC155000.030.78871219
CAGAGTA170750.028.14641418
CAGTGGT174800.028.0992574
GCAGAGT175200.027.72688317
GCAGTGG178850.027.551023
AGCAGTG180850.027.0970422
ACATGGG113050.026.96815524
Produced by FastQC (version 0.11.5)