##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805301_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11278606
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.28354505867126	37.0	35.0	37.0	33.0	37.0
2	35.3636529195186	37.0	35.0	37.0	33.0	37.0
3	37.28690664431402	39.0	37.0	39.0	34.0	39.0
4	37.43515156039674	39.0	37.0	39.0	34.0	39.0
5	37.490006211760566	39.0	37.0	39.0	35.0	39.0
6	37.494772935591506	39.0	37.0	39.0	35.0	39.0
7	37.45651758736851	39.0	37.0	39.0	35.0	39.0
8	38.294847519276765	40.0	38.0	40.0	34.0	40.0
9	38.25667524869652	40.0	38.0	40.0	34.0	40.0
10	38.25896666662529	40.0	38.0	40.0	34.0	40.0
11	38.17146436359245	40.0	38.0	40.0	34.0	40.0
12	38.082085498863954	40.0	38.0	40.0	34.0	40.0
13	38.16276798746228	40.0	38.0	40.0	34.0	40.0
14	38.053007614593504	40.0	38.0	40.0	34.0	40.0
15	37.98157680124654	40.0	38.0	40.0	34.0	40.0
16	37.933448335725174	40.0	38.0	40.0	34.0	40.0
17	37.83624882365782	40.0	38.0	40.0	34.0	40.0
18	37.733662475664104	40.0	37.0	40.0	33.0	40.0
19	37.61383020206575	40.0	37.0	40.0	33.0	40.0
20	37.39524095442292	39.0	37.0	40.0	33.0	40.0
21	37.262266099197014	39.0	37.0	40.0	32.0	40.0
22	36.23342485764641	39.0	36.0	40.0	30.0	40.0
23	36.613741095309116	39.0	36.0	40.0	31.0	40.0
24	36.57588109736256	38.0	36.0	40.0	31.0	40.0
25	36.51790371966181	38.0	36.0	40.0	31.0	40.0
26	36.37266653343507	38.0	36.0	40.0	30.0	40.0
27	36.21438881719957	38.0	35.0	40.0	30.0	40.0
28	36.08921315276019	38.0	35.0	40.0	30.0	40.0
29	35.955134615040194	38.0	35.0	40.0	30.0	40.0
30	35.69987328221236	38.0	35.0	40.0	30.0	40.0
31	35.10837279004161	38.0	34.0	39.0	27.0	40.0
32	35.14484210194061	38.0	34.0	39.0	28.0	40.0
33	35.060236256147256	37.0	34.0	39.0	29.0	40.0
34	34.93504427763502	37.0	34.0	39.0	28.0	40.0
35	34.740884644786775	37.0	33.0	39.0	27.0	40.0
36	34.521286407203156	37.0	33.0	38.0	27.0	40.0
37	34.25730635505842	36.0	33.0	38.0	27.0	40.0
38	34.01820331342366	36.0	32.0	38.0	26.0	40.0
39	33.75168855087233	36.0	32.0	38.0	25.0	39.0
40	33.44684405147232	35.0	31.0	38.0	25.0	39.0
41	33.39055181110148	35.0	31.0	38.0	25.0	39.0
42	33.210701482080324	35.0	31.0	38.0	24.0	39.0
43	33.08611835540669	35.0	31.0	38.0	24.0	39.0
44	32.853070672031635	35.0	31.0	38.0	24.0	39.0
45	32.504990776342396	35.0	30.0	38.0	23.0	39.0
46	32.257728393030135	34.0	30.0	38.0	23.0	39.0
47	31.997572838345448	34.0	30.0	37.0	23.0	39.0
48	31.667003528627564	34.0	29.0	37.0	22.0	38.0
49	31.128279682790588	33.0	29.0	37.0	20.0	38.0
50	30.890618486007934	33.0	28.0	37.0	19.0	38.0
51	32.13655286832433	34.0	30.0	37.0	23.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	13.0
8	65.0
9	128.0
10	217.0
11	380.0
12	823.0
13	1342.0
14	2111.0
15	3060.0
16	4557.0
17	6532.0
18	9837.0
19	14581.0
20	21575.0
21	31038.0
22	43660.0
23	58666.0
24	76837.0
25	96583.0
26	120260.0
27	144565.0
28	173342.0
29	205148.0
30	241543.0
31	287589.0
32	348134.0
33	439273.0
34	595654.0
35	918780.0
36	1777280.0
37	3468275.0
38	2122295.0
39	64463.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.067305303510025	27.36122708781564	26.524297417606395	31.04717019106794
2	25.015866322487017	24.71042963997501	29.56723552538319	20.706468512154782
3	17.449798317274315	24.956488417096935	31.709725474939017	25.883987790689737
4	18.40915446465636	27.495055683299867	30.8055445859178	23.29024526612597
5	23.76284799735003	27.572024415073994	28.476223036783093	20.18890455079289
6	22.942932841168492	26.211076085111934	28.953844118679207	21.892146955040364
7	23.495527727451424	26.877860615044092	28.549884622266262	21.07672703523822
8	22.196058626394077	25.29450004725761	28.79540255240763	23.71403877394068
9	22.23929091946292	24.74076140260596	29.931252142330354	23.088695535600763
10	22.66481336434662	25.79246938850422	28.305306524582914	23.237410722566246
11	22.474568222349465	27.054433854680266	27.4371052592847	23.033892663685567
12	22.566459010980612	26.180105945717052	28.095715020100887	23.15772002320145
13	22.80827967569751	26.339966127019597	26.773273221885752	24.078480975397138
14	23.07137069953503	27.155057992095834	26.663020234947478	23.11055107342166
15	22.781077732478643	27.1420067338109	26.754183983375253	23.32273155033521
16	23.108733472913233	26.276084118906184	26.435864503113237	24.179317905067347
17	24.354676455583252	25.978662611319166	26.450892956097587	23.215767976999995
18	23.175266517865772	25.96471585229593	26.405559339514124	24.454458290324176
19	23.353249506188973	26.833573227045964	26.3186603025232	23.494516964241857
20	24.253280946244598	26.042526886744692	26.309332908694564	23.394859258316142
21	23.325054532448426	26.8898479120558	26.404122991795266	23.380974563700512
22	24.334806978805716	25.66521075388217	26.348867936338944	23.651114330973172
23	23.313457354570236	26.03193160573213	27.423876674120894	23.230734365576737
24	23.124072247935604	26.863036087970443	26.506759789286015	23.506131874807934
25	23.136742253430963	25.998780345727123	26.376921048576392	24.487556352265518
26	23.30616035350468	26.512407650378066	26.849798636462697	23.331633359654553
27	23.20977432849414	25.851368511321343	27.496855551120415	23.442001609064096
28	23.918904517100785	25.855464762223274	26.643638407086833	23.581992313589108
29	24.059622261829166	25.93965069796746	26.643221688921486	23.357505351281887
30	23.87231187967733	25.8177207360555	26.723905418807963	23.58606196545921
31	23.82785603114427	25.733738726222015	26.538980083176945	23.899425159456765
32	23.781139264905608	26.16832257461605	26.56940050924733	23.481137651231013
33	23.546597868566383	26.064896672514315	26.637485164389997	23.751020294529308
34	23.627201801357366	26.070810523924674	26.487103104763126	23.814884569954835
35	23.681685484890597	26.115470298368432	26.576688643968943	23.626155572772024
36	23.545276783318787	26.048103817085195	26.6265174969318	23.780101902664214
37	23.64781605102616	26.06447995434897	26.424409186738146	23.86329480788672
38	23.833220169230135	26.15267347755565	26.545434781567863	23.468671571646354
39	23.611960556118373	26.01802917842861	26.632005763832872	23.738004501620146
40	23.656966117975927	26.05991378721803	26.458908131022575	23.82421196378347
41	23.72380061862255	26.113466504637184	26.60164740217009	23.561085474570174
42	23.533644140064826	26.00968594877771	26.728108065837215	23.728561845320247
43	23.628762277891436	25.995224941805752	26.527985816686922	23.848026963615894
44	23.76677578771703	26.01099816768136	26.59207175071104	23.630154293890573
45	23.620117592546457	25.85900243345676	26.76384829827374	23.757031675723045
46	23.678990116331754	25.937309983166358	26.51482816227466	23.868871738227224
47	23.7500627293834	25.983698694679113	26.55893822339392	23.707300352543566
48	23.5680100891901	25.87661985887263	26.682295666680794	23.873074385256476
49	23.912423219678033	25.90074518074308	26.427946857971634	23.758884741607254
50	23.770251394542907	26.02766689429527	26.54707505519742	23.655006655964396
51	23.673138329328996	25.946841302905693	26.598357988567027	23.78166237919828
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3143.0
1	2218.5
2	1294.0
3	1146.0
4	998.0
5	1016.5
6	1035.0
7	1198.0
8	1361.0
9	1768.0
10	2175.0
11	3135.0
12	4095.0
13	5662.0
14	7229.0
15	10133.0
16	13037.0
17	18649.0
18	24261.0
19	32486.0
20	40711.0
21	52915.0
22	65119.0
23	96458.5
24	127798.0
25	134728.0
26	163048.0
27	184438.0
28	207754.0
29	231070.0
30	255598.5
31	280127.0
32	306912.0
33	333697.0
34	368467.0
35	403237.0
36	446931.5
37	490626.0
38	527025.5
39	563425.0
40	604588.5
41	645752.0
42	679238.0
43	712724.0
44	743298.5
45	773873.0
46	796045.5
47	818218.0
48	825436.5
49	832655.0
50	833374.0
51	834093.0
52	817395.5
53	800698.0
54	762998.5
55	725299.0
56	673262.5
57	621226.0
58	564051.0
59	506876.0
60	445683.0
61	384490.0
62	328965.0
63	273440.0
64	229306.5
65	185173.0
66	147377.0
67	109581.0
68	88554.0
69	67527.0
70	51064.5
71	34602.0
72	25208.0
73	15814.0
74	11900.0
75	5970.0
76	3954.0
77	2965.5
78	1977.0
79	1540.5
80	1104.0
81	899.0
82	694.0
83	459.5
84	225.0
85	142.0
86	59.0
87	41.0
88	23.0
89	15.0
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.1278606E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.33164314565845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.71710144936881	48.891808186989394
2	11.61962734628725	14.253016757760479
3	3.5654640008786105	6.560272972517356
4	1.5946342507263895	3.9120615525358144
5	0.8573343343380221	2.6290861725070096
6	0.5464460578148274	2.01086607697505
7	0.3806573269665905	1.6342437536806607
8	0.27219307184087854	1.3355238679092267
9	0.21358177783136587	1.178938924233177
>10	1.1486368860735503	12.571867252960555
>50	0.065362802952765	2.72076441747449
>100	0.018665996626687723	1.880566027420239
>500	1.8633132420114296E-4	0.06843581394297221
>1k	9.288604548471089E-5	0.11385133096263526
>5k	0.0	0.0
>10k+	1.548092444354379E-5	0.23869689213103307
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25140	0.2228998867413225	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.09307515485513E-5	0.0	0.0	0.0	4.4331719717844566E-5
2	7.979709549212021E-5	8.866343943568912E-6	0.0	0.0	4.4331719717844566E-5
3	8.866343943568913E-5	8.866343943568912E-6	0.0	8.866343943568912E-6	5.3198063661413477E-5
4	8.866343943568913E-5	8.866343943568912E-6	0.0	8.866343943568912E-6	5.3198063661413477E-5
5	3.5465375774275653E-4	8.866343943568912E-6	0.0	8.866343943568912E-6	7.09307515485513E-5
6	3.5465375774275653E-4	1.7732687887137824E-5	0.0	8.866343943568912E-6	9.752978337925804E-5
7	3.5465375774275653E-4	1.7732687887137824E-5	0.0	8.866343943568912E-6	1.0639612732282695E-4
8	3.5465375774275653E-4	1.7732687887137824E-5	0.0	8.866343943568912E-6	1.1526247126639587E-4
9	3.5465375774275653E-4	1.7732687887137824E-5	0.0	8.866343943568912E-6	1.241288152099648E-4
10	3.8125278957346325E-4	1.7732687887137824E-5	0.0	8.866343943568912E-6	1.241288152099648E-4
11	3.9011913351703214E-4	1.7732687887137824E-5	0.0	4.4331719717844566E-5	1.241288152099648E-4
12	4.0785182140417E-4	1.7732687887137824E-5	0.0	4.4331719717844566E-5	1.418615030971026E-4
13	4.965152608398592E-4	2.6599031830706738E-5	0.0	4.4331719717844566E-5	1.7732687887137826E-4
14	5.231142926705658E-4	2.6599031830706738E-5	0.0	4.4331719717844566E-5	2.03925910702085E-4
15	5.674460123884104E-4	2.6599031830706738E-5	0.0	4.4331719717844566E-5	2.03925910702085E-4
16	6.02911388162686E-4	2.6599031830706738E-5	0.0	4.4331719717844566E-5	2.3052494253279174E-4
17	6.383767639369617E-4	2.6599031830706738E-5	0.0	4.4331719717844566E-5	2.5712397436349847E-4
18	7.093075154855131E-4	2.6599031830706738E-5	0.0	5.3198063661413477E-5	2.5712397436349847E-4
19	7.447728912597886E-4	2.6599031830706738E-5	0.0	5.3198063661413477E-5	2.6599031830706736E-4
20	7.713719230904954E-4	2.6599031830706738E-5	0.0	7.09307515485513E-5	2.9258935013777413E-4
21	8.1570364280834E-4	2.6599031830706738E-5	0.0	7.09307515485513E-5	3.1032203802491197E-4
22	8.423026746390467E-4	2.6599031830706738E-5	0.0	7.09307515485513E-5	3.369210698556187E-4
23	8.777680504133224E-4	2.6599031830706738E-5	0.0	7.09307515485513E-5	3.635201016863254E-4
24	9.398324580183047E-4	2.6599031830706738E-5	0.0	7.09307515485513E-5	3.635201016863254E-4
25	9.930305216797183E-4	2.6599031830706738E-5	0.0	7.09307515485513E-5	3.635201016863254E-4
26	0.0010728276171718385	2.6599031830706738E-5	0.0	7.979709549212021E-5	3.635201016863254E-4
27	0.0011348920247768208	2.6599031830706738E-5	0.0	7.979709549212021E-5	3.723864456298943E-4
28	0.0011614910566075276	2.6599031830706738E-5	0.0	7.979709549212021E-5	3.8125278957346325E-4
29	0.001214689120268941	3.546537577427565E-5	0.0	8.866343943568913E-5	3.8125278957346325E-4
30	0.001312218903648199	3.546537577427565E-5	0.0	8.866343943568913E-5	3.8125278957346325E-4
31	0.0013654169673096126	3.546537577427565E-5	0.0	8.866343943568913E-5	3.989854774606011E-4
32	0.0014186150309710261	3.546537577427565E-5	0.0	8.866343943568913E-5	4.167181653477389E-4
33	0.001525011158293853	4.4331719717844566E-5	0.0	9.752978337925804E-5	4.6991622900915237E-4
34	0.0015870755658988355	4.4331719717844566E-5	0.0	9.752978337925804E-5	4.876489168962902E-4
35	0.001728937068995938	4.4331719717844566E-5	0.0	9.752978337925804E-5	5.05381604783428E-4
36	0.0018619322281494717	4.4331719717844566E-5	0.0	1.1526247126639587E-4	5.319806366141347E-4
37	0.0019594620115287297	4.4331719717844566E-5	0.0	1.241288152099648E-4	5.408469805577037E-4
38	0.002065858138851557	4.4331719717844566E-5	0.0	1.241288152099648E-4	5.408469805577037E-4
39	0.002136788890400108	5.3198063661413477E-5	0.0	1.3299515915353368E-4	5.763123563319793E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	13960	0.0	35.071632	12
CAACGCA	13970	0.0	35.030422	13
AACGCAG	14410	0.0	33.99202	14
ACGCAGA	14430	0.0	33.94491	15
GTATCAA	14870	0.0	32.864826	9
GGTATCA	14900	0.0	32.82886	8
ATCAACG	15045	0.0	32.557327	11
AGTGGTA	15285	0.0	32.001965	5
GTGGTAT	15300	0.0	31.941177	6
CGCAGAG	15260	0.0	31.700521	16
TGGTATC	15490	0.0	31.607489	7
AAGCAGT	15560	0.0	31.161633	1
TATCAAC	15790	0.0	31.00697	10
AGAGTAC	15500	0.0	30.788712	19
CAGAGTA	17075	0.0	28.146414	18
CAGTGGT	17480	0.0	28.099257	4
GCAGAGT	17520	0.0	27.726883	17
GCAGTGG	17885	0.0	27.55102	3
AGCAGTG	18085	0.0	27.097042	2
ACATGGG	11305	0.0	26.968155	24
>>END_MODULE