FastQCFastQC Report
Thu 26 May 2016
SRR805300_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805300_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27357190
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7569792.7670202970407414No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT479050.17510935881938167No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG394660.14426189239465018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC2297450.042.2411819
CAACGCA2396100.042.1585113
TCAACGC2397950.042.128812
AACGCAG2415200.041.8083414
GTATCAA2413400.041.8040969
GGTATCA2415400.041.7806638
ATCAACG2415500.041.7761411
ACGCAGA2415700.041.7754715
AAGCAGT2430650.041.7425351
TGGTATC2424450.041.6451157
GTGGTAT2426050.041.6195076
CGCAGAG2426450.041.61171716
AGTGGTA2422650.041.5989765
TATCAAC2438900.041.37162810
GAGTACT987650.041.14767520
GTACATG1357300.041.03975322
ACATGGG1344350.040.9999324
GAGTACA1376500.040.87595720
CAGAGTA2391100.040.8718818
CAGTGGT2481400.040.773664
Produced by FastQC (version 0.11.5)