FastQCFastQC Report
Thu 26 May 2016
SRR805299_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805299_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28976113
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT879680.3035879933240183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT425200.033.3795851
TCAACGC452150.031.55921712
CAACGCA453850.031.406313
AACGCAG464450.030.71859414
ACGCAGA467150.030.55549615
GGTATCA469200.030.3884268
GTGGTAT470650.030.3139276
GTATCAA471000.030.277079
AGTGGTA472400.030.1730545
TGGTATC473200.030.1458157
ATCAACG480650.029.6738811
TATCAAC490400.029.15273310
CGCAGAG491350.029.06431216
AGAGTAC496050.027.99516119
AGCAGTG521150.027.3073982
CAGTGGT526750.027.230664
GCAGTGG532100.026.9188123
CAGAGTA535700.026.08269518
GCAGAGT551300.025.920117
GTACATG360350.024.86332922
Produced by FastQC (version 0.11.5)