##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805299_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28976113
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.38332615558201	37.0	37.0	37.0	35.0	37.0
2	36.322352794524235	37.0	37.0	37.0	35.0	37.0
3	38.123639599279585	39.0	39.0	39.0	37.0	39.0
4	38.116581061096774	39.0	39.0	39.0	37.0	39.0
5	38.12275386971331	39.0	39.0	39.0	37.0	39.0
6	38.07508705532726	39.0	39.0	39.0	37.0	39.0
7	38.055534674371266	39.0	39.0	39.0	37.0	39.0
8	39.01881460084036	40.0	40.0	40.0	37.0	40.0
9	38.98648452261351	40.0	40.0	40.0	37.0	40.0
10	38.98946635803084	40.0	40.0	40.0	37.0	40.0
11	38.955922314355966	40.0	40.0	40.0	37.0	40.0
12	38.92542019007173	40.0	40.0	40.0	37.0	40.0
13	38.977823630105256	40.0	40.0	40.0	37.0	40.0
14	38.93217168224047	40.0	40.0	40.0	37.0	40.0
15	38.90438310342039	40.0	40.0	40.0	37.0	40.0
16	38.84729791052375	40.0	39.0	40.0	36.0	40.0
17	38.81714434920929	40.0	39.0	40.0	36.0	40.0
18	38.78322931029431	40.0	39.0	40.0	36.0	40.0
19	38.73535039016448	40.0	39.0	40.0	36.0	40.0
20	38.62975641349825	40.0	39.0	40.0	36.0	40.0
21	38.575373480908226	40.0	39.0	40.0	36.0	40.0
22	38.54531009732051	40.0	39.0	40.0	36.0	40.0
23	38.46400008862472	40.0	39.0	40.0	35.0	40.0
24	38.061900952691616	40.0	38.0	40.0	34.0	40.0
25	38.35421393476758	40.0	39.0	40.0	35.0	40.0
26	38.42115075959291	40.0	39.0	40.0	35.0	40.0
27	38.41417729148144	40.0	39.0	40.0	35.0	40.0
28	38.408681799384205	40.0	39.0	40.0	35.0	40.0
29	38.45766424916965	40.0	39.0	40.0	35.0	40.0
30	38.46396119451909	40.0	39.0	40.0	35.0	40.0
31	38.39648416611296	40.0	39.0	40.0	35.0	40.0
32	38.41053970903551	40.0	39.0	40.0	35.0	40.0
33	38.37416305630779	40.0	39.0	40.0	35.0	40.0
34	38.379519847952004	40.0	39.0	40.0	35.0	40.0
35	38.3360880736488	40.0	39.0	40.0	35.0	40.0
36	38.27490819765922	40.0	39.0	40.0	35.0	40.0
37	38.236519232237946	40.0	39.0	40.0	35.0	40.0
38	38.182706493448585	40.0	39.0	40.0	35.0	40.0
39	38.14220992995161	40.0	38.0	40.0	35.0	40.0
40	38.072482634230475	40.0	38.0	40.0	34.0	40.0
41	37.97973472149284	40.0	38.0	40.0	34.0	40.0
42	37.90555051327968	40.0	38.0	40.0	34.0	40.0
43	37.877274532992054	40.0	38.0	40.0	34.0	40.0
44	37.808796542172516	40.0	38.0	40.0	34.0	40.0
45	37.61872436099348	40.0	38.0	40.0	33.0	40.0
46	37.57274189950874	40.0	37.0	40.0	33.0	40.0
47	37.53229133942154	40.0	37.0	40.0	33.0	40.0
48	37.418167647261726	40.0	37.0	40.0	33.0	40.0
49	37.341180302547826	40.0	37.0	40.0	33.0	40.0
50	37.26401098035475	40.0	37.0	40.0	33.0	40.0
51	36.73846005501152	39.0	36.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	78.0
9	236.0
10	418.0
11	511.0
12	553.0
13	719.0
14	886.0
15	1218.0
16	2002.0
17	3372.0
18	5994.0
19	10021.0
20	17030.0
21	25791.0
22	37218.0
23	50750.0
24	67067.0
25	86509.0
26	108439.0
27	133727.0
28	163923.0
29	200193.0
30	244298.0
31	295377.0
32	359824.0
33	444328.0
34	568080.0
35	772506.0
36	1122493.0
37	1867708.0
38	4112098.0
39	1.827274E7
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.45294253235415	29.217431613412053	22.43917256948853	25.89045328474527
2	24.901455899209118	23.82463790088063	29.174889675506165	22.09901652440408
3	21.12540077407898	25.41075471371885	27.586170719309383	25.877673792892786
4	21.761051939575193	28.102699627103195	26.698594804624072	23.43765362869754
5	25.221871546400997	27.903183563647755	25.157073345206793	21.71787154474446
6	24.611582650854515	25.745143939768596	27.129656762451194	22.513616646925694
7	23.62257836308134	26.65514867366786	27.34922727558386	22.37304568766694
8	23.39616083081951	25.61831188330885	27.36592723806675	23.61960004780489
9	23.327659579461194	25.576729356349485	27.883791728724965	23.211819335464355
10	22.732144922267526	26.3182263266298	27.952538009497687	22.99709074160499
11	22.655019325746004	27.291907648206642	27.001330371675458	23.051742654371896
12	22.699566363507763	26.058950695008676	27.817119570178374	23.424363371305184
13	23.04911290206523	26.385322972753457	26.388356505926104	24.17720761925521
14	23.11727249269079	27.301194608124284	26.418995536081734	23.16253736310319
15	23.08263706729747	27.10521594114435	26.367394412080046	23.444752579478138
16	23.3402561620325	26.164793048674266	26.233987974853633	24.260962814439605
17	24.438395170532363	26.0118153183624	26.25886708821159	23.290922422893644
18	23.298770266391493	25.975740776549287	26.159188432209664	24.566300524849556
19	23.494024198483764	27.04459704446901	26.15576147152656	23.305617285520665
20	24.43254552465336	26.041243005920084	26.23746670231442	23.28874476711214
21	23.467278030010444	26.870332815170894	26.267163577116087	23.395225577702572
22	24.434712827079323	25.93808907357588	26.28633108933555	23.340867010009244
23	23.364086135362598	26.041039389927832	27.376781005789148	23.21809346892042
24	23.438688964251348	26.608220364132347	26.142902603948293	23.810188067668015
25	23.379005320692944	26.007501420221544	26.180902869891487	24.432590389194022
26	23.45830857299597	26.561143656500786	26.740877908641508	23.239669861861735
27	23.454784980994518	25.752764009444608	27.33195097630935	23.460500033251527
28	24.228415315746457	25.943773065766273	26.490399178109225	23.337412440378046
29	24.229747447492354	25.910269607245112	26.56988533969342	23.29009760556911
30	24.113575896118295	25.808589302505823	26.505249341069316	23.572585460306563
31	23.917628289204973	26.06419639514796	26.474555093017482	23.543620222629585
32	23.819002224349415	26.19156337497717	26.49473723407967	23.49469716659374
33	23.691538613201846	26.004457533693355	26.534576946190125	23.76942690691467
34	23.781543784012715	26.145463333884706	26.440737582711666	23.632255299390916
35	23.768391571360866	26.133091074016722	26.49447840019122	23.60403895443119
36	23.683507860422825	26.06021380438432	26.44516536776344	23.811112967429413
37	23.82688112791388	26.16262919736681	26.307434679040632	23.70305499567868
38	23.83162296475031	26.177365473415982	26.448550915024388	23.54246064680932
39	23.782679202003386	26.04397629178213	26.415295937036138	23.758048569178346
40	23.836975649563488	26.157814887041614	26.30607493834663	23.699134525048272
41	23.71691123650712	26.186807733666694	26.52650477998895	23.569776249837236
42	23.719423650784353	26.053987986587433	26.51314204910783	23.713446313520382
43	23.86632741251389	26.112173844711332	26.279301160925208	23.742197581849574
44	23.809787737920544	26.031359002499748	26.46895392767139	23.68989933190832
45	23.820348160569363	25.828405625005672	26.614228761462932	23.737017452962032
46	23.80706135429552	26.085382811697343	26.398568365605147	23.70898746840199
47	23.791707328032576	26.063406089008556	26.428151353495892	23.71673522946297
48	23.714443686770547	25.955065125539782	26.45004870045889	23.880442487230773
49	23.86547153512274	26.17564336527815	26.23691452335239	23.72197057624672
50	23.865530204137457	26.13002302965895	26.3741551532464	23.630291612957198
51	23.785578141554044	25.976061730570972	26.414381390630275	23.82397873724471
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15599.0
1	9994.5
2	4390.0
3	4002.5
4	3615.0
5	3919.0
6	4223.0
7	4663.5
8	5104.0
9	6154.5
10	7205.0
11	9846.5
12	12488.0
13	16696.5
14	20905.0
15	27839.0
16	34773.0
17	49325.0
18	63877.0
19	85449.5
20	107022.0
21	138774.5
22	170527.0
23	262238.5
24	353950.0
25	359652.5
26	421168.0
27	476981.0
28	533185.5
29	589390.0
30	639045.0
31	688700.0
32	751431.5
33	814163.0
34	892681.5
35	971200.0
36	1075319.0
37	1179438.0
38	1263838.5
39	1348239.0
40	1448192.0
41	1548145.0
42	1634467.5
43	1720790.0
44	1801555.0
45	1882320.0
46	1946756.0
47	2011192.0
48	2041216.5
49	2071241.0
50	2096839.5
51	2122438.0
52	2109701.0
53	2096964.0
54	2010475.0
55	1923986.0
56	1805875.5
57	1687765.0
58	1542897.5
59	1398030.0
60	1247022.5
61	1096015.0
62	959296.0
63	822577.0
64	705806.5
65	589036.0
66	466304.5
67	343573.0
68	285389.0
69	227205.0
70	168447.0
71	109689.0
72	77913.0
73	46137.0
74	33793.0
75	15910.0
76	10371.0
77	7545.5
78	4720.0
79	3594.5
80	2469.0
81	2044.5
82	1620.0
83	1147.0
84	674.0
85	463.5
86	253.0
87	192.0
88	131.0
89	106.5
90	82.0
91	62.0
92	42.0
93	41.0
94	40.0
95	26.0
96	12.0
97	7.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.8976113E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.70541312669546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.89242862788	31.24569896237452
2	15.556686780753681	13.90936218832391
3	5.756387775424777	7.720250808534725
4	2.702343683991016	4.832375632125383
5	1.5131503729771674	3.38230062733788
6	0.9153697310388854	2.455318919385929
7	0.6305733263766452	1.9733028743639751
8	0.47153007069799446	1.6863957289771012
9	0.33613223889641325	1.3524237544558724
>10	1.9691962321819383	17.156610431477425
>50	0.17472747996542723	5.316914370674207
>100	0.07590275555892805	6.151904388999637
>500	0.003497256139567302	1.086650125706595
>1k	0.002041788990644332	1.310358333081337
>5k	7.96978172830186E-6	0.02483357921312466
>10k+	2.390934518482159E-5	0.39529927496816014
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	87968	0.3035879933240183	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2785625870523075E-4	0.0	0.0	1.035335553805992E-5	6.902237025373279E-6
2	3.313073772179174E-4	0.0	0.0	1.035335553805992E-5	6.902237025373279E-6
3	3.313073772179174E-4	0.0	0.0	1.035335553805992E-5	6.902237025373279E-6
4	3.313073772179174E-4	0.0	0.0	1.035335553805992E-5	1.035335553805992E-5
5	3.382096142432907E-4	0.0	0.0	1.7255592563433197E-5	1.7255592563433197E-5
6	3.4511185126866395E-4	0.0	0.0	2.070671107611984E-5	2.070671107611984E-5
7	3.4511185126866395E-4	0.0	0.0	2.070671107611984E-5	2.4157829588806477E-5
8	3.4511185126866395E-4	0.0	0.0	2.4157829588806477E-5	2.4157829588806477E-5
9	3.4511185126866395E-4	0.0	3.4511185126866393E-6	2.4157829588806477E-5	2.7608948101493115E-5
10	3.485629697813506E-4	0.0	3.4511185126866393E-6	3.4511185126866394E-5	3.106006661417975E-5
11	3.589163253194105E-4	0.0	3.4511185126866393E-6	3.7962303639553036E-5	3.4511185126866394E-5
12	3.6236744383209715E-4	0.0	3.4511185126866393E-6	4.486454066492631E-5	4.486454066492631E-5
13	3.6581856234478376E-4	0.0	3.4511185126866393E-6	4.486454066492631E-5	5.521789620298623E-5
14	3.761719178828437E-4	0.0	3.4511185126866393E-6	4.486454066492631E-5	5.521789620298623E-5
15	3.7962303639553036E-4	3.4511185126866393E-6	3.4511185126866393E-6	5.521789620298623E-5	5.521789620298623E-5
16	3.899763919335903E-4	3.4511185126866393E-6	3.4511185126866393E-6	5.866901471567287E-5	8.282684430447935E-5
17	4.1758534003508336E-4	3.4511185126866393E-6	3.4511185126866393E-6	6.21201332283595E-5	1.0698467389328582E-4
18	4.4864540664926316E-4	3.4511185126866393E-6	3.4511185126866393E-6	6.557125174104615E-5	1.0698467389328582E-4
19	4.6935211772538296E-4	3.4511185126866393E-6	3.4511185126866393E-6	6.902237025373279E-5	1.138869109186591E-4
20	5.142166583903092E-4	3.4511185126866393E-6	3.4511185126866393E-6	6.902237025373279E-5	1.138869109186591E-4
21	5.763367916186688E-4	3.4511185126866393E-6	3.4511185126866393E-6	7.247348876641944E-5	1.2078914794403239E-4
22	6.177502137709084E-4	3.4511185126866393E-6	3.4511185126866393E-6	7.592460727910607E-5	1.311425034820923E-4
23	6.936748210500146E-4	6.902237025373279E-6	3.4511185126866393E-6	7.93757257917927E-5	1.3459362199477894E-4
24	7.695994283291206E-4	6.902237025373279E-6	3.4511185126866393E-6	8.282684430447935E-5	1.414958590201522E-4
25	8.524262726335999E-4	1.035335553805992E-5	3.4511185126866393E-6	8.282684430447935E-5	1.414958590201522E-4
26	9.387042354507659E-4	1.035335553805992E-5	3.4511185126866393E-6	8.282684430447935E-5	1.414958590201522E-4
27	0.0010629445019074849	1.035335553805992E-5	3.4511185126866393E-6	8.627796281716599E-5	1.4494697753283887E-4
28	0.0011354179906739043	1.035335553805992E-5	3.4511185126866393E-6	8.972908132985262E-5	1.5530033307089878E-4
29	0.0012665604941559966	1.035335553805992E-5	3.4511185126866393E-6	8.972908132985262E-5	1.6220257009627205E-4
30	0.001397702997638089	1.035335553805992E-5	3.4511185126866393E-6	8.972908132985262E-5	1.6910480712164534E-4
31	0.0015357477381455545	1.035335553805992E-5	3.4511185126866393E-6	8.972908132985262E-5	1.760070441470186E-4
32	0.0017083036637798865	1.035335553805992E-5	3.4511185126866393E-6	1.0008243686791254E-4	1.7945816265970525E-4
33	0.0018877618264395918	1.035335553805992E-5	3.4511185126866393E-6	1.0353355538059919E-4	1.8636039968507854E-4
34	0.002039611040997804	1.035335553805992E-5	3.4511185126866393E-6	1.0353355538059919E-4	1.8636039968507854E-4
35	0.002184558018530643	1.035335553805992E-5	3.4511185126866393E-6	1.0698467389328582E-4	1.8981151819776518E-4
36	0.002326053877550795	1.3804474050746557E-5	3.4511185126866393E-6	1.138869109186591E-4	2.0361599224851174E-4
37	0.00251586539574856	1.3804474050746557E-5	3.4511185126866393E-6	1.1733802943134574E-4	2.0361599224851174E-4
38	0.002802308232301551	1.3804474050746557E-5	6.902237025373279E-6	1.2078914794403239E-4	2.0361599224851174E-4
39	0.003112908898443349	1.3804474050746557E-5	6.902237025373279E-6	1.2078914794403239E-4	2.10518229273885E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	42520	0.0	33.379585	1
TCAACGC	45215	0.0	31.559217	12
CAACGCA	45385	0.0	31.4063	13
AACGCAG	46445	0.0	30.718594	14
ACGCAGA	46715	0.0	30.555496	15
GGTATCA	46920	0.0	30.388426	8
GTGGTAT	47065	0.0	30.313927	6
GTATCAA	47100	0.0	30.27707	9
AGTGGTA	47240	0.0	30.173054	5
TGGTATC	47320	0.0	30.145815	7
ATCAACG	48065	0.0	29.67388	11
TATCAAC	49040	0.0	29.152733	10
CGCAGAG	49135	0.0	29.064312	16
AGAGTAC	49605	0.0	27.995161	19
AGCAGTG	52115	0.0	27.307398	2
CAGTGGT	52675	0.0	27.23066	4
GCAGTGG	53210	0.0	26.918812	3
CAGAGTA	53570	0.0	26.082695	18
GCAGAGT	55130	0.0	25.9201	17
GTACATG	36035	0.0	24.863329	22
>>END_MODULE