FastQCFastQC Report
Thu 26 May 2016
SRR805298_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805298_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19935942
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT679790.340987147735482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC314050.035.8796412
CAACGCA316050.035.6881813
AACGCAG322050.034.9813714
ACGCAGA322600.034.88685615
GGTATCA331000.033.974328
ATCAACG332900.033.86827511
GTATCAA333350.033.734819
AAGCAGT332500.033.651881
GTGGTAT337200.033.342976
CGCAGAG338150.033.2892216
TGGTATC338350.033.2296457
AGTGGTA339300.033.0901835
AGAGTAC331950.032.4333519
TATCAAC348000.032.3534510
CAGAGTA358350.030.17580618
CAGTGGT373900.030.142424
GCAGAGT374200.030.01603517
GCAGTGG383200.029.3873963
AGCAGTG388100.028.9525892
GTACATG240400.028.85503222
Produced by FastQC (version 0.11.5)