##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805298_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19935942
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.98527568950592	37.0	37.0	37.0	35.0	37.0
2	36.050980736199975	37.0	37.0	37.0	35.0	37.0
3	37.90458589817326	39.0	39.0	39.0	35.0	39.0
4	37.95728829869188	39.0	39.0	39.0	35.0	39.0
5	37.98792136333462	39.0	39.0	39.0	35.0	39.0
6	37.97086749148849	39.0	39.0	39.0	35.0	39.0
7	37.957560420270084	39.0	39.0	39.0	35.0	39.0
8	38.90715297024841	40.0	40.0	40.0	37.0	40.0
9	38.88218861190507	40.0	40.0	40.0	36.0	40.0
10	38.869248867196745	40.0	39.0	40.0	36.0	40.0
11	38.8426243415034	40.0	39.0	40.0	36.0	40.0
12	38.82097640532863	40.0	39.0	40.0	36.0	40.0
13	38.870837906731474	40.0	40.0	40.0	36.0	40.0
14	38.83044217323666	40.0	39.0	40.0	36.0	40.0
15	38.802350498411364	40.0	39.0	40.0	36.0	40.0
16	38.75748951316171	40.0	39.0	40.0	36.0	40.0
17	38.72525210998307	40.0	39.0	40.0	36.0	40.0
18	38.68055936358563	40.0	39.0	40.0	36.0	40.0
19	38.6202691099322	40.0	39.0	40.0	36.0	40.0
20	38.505882039584584	40.0	39.0	40.0	36.0	40.0
21	38.44674202001591	40.0	39.0	40.0	35.0	40.0
22	38.413019209225226	40.0	39.0	40.0	35.0	40.0
23	38.29890014728173	40.0	38.0	40.0	35.0	40.0
24	37.73764605655454	40.0	38.0	40.0	33.0	40.0
25	38.15503350681899	40.0	38.0	40.0	34.0	40.0
26	38.2596459199169	40.0	38.0	40.0	35.0	40.0
27	38.282925482026386	40.0	39.0	40.0	35.0	40.0
28	38.28762538534673	40.0	39.0	40.0	35.0	40.0
29	38.32579574117943	40.0	39.0	40.0	35.0	40.0
30	38.33113017684341	40.0	39.0	40.0	35.0	40.0
31	38.250855765932705	40.0	39.0	40.0	35.0	40.0
32	38.284636161160584	40.0	39.0	40.0	35.0	40.0
33	38.253519898884136	40.0	39.0	40.0	35.0	40.0
34	38.252827982745934	40.0	39.0	40.0	35.0	40.0
35	38.20062738946572	40.0	39.0	40.0	35.0	40.0
36	38.13907940743407	40.0	39.0	40.0	35.0	40.0
37	38.09998975719331	40.0	38.0	40.0	34.0	40.0
38	38.047087315964305	40.0	38.0	40.0	34.0	40.0
39	38.02005297768222	40.0	38.0	40.0	34.0	40.0
40	37.94677146432308	40.0	38.0	40.0	34.0	40.0
41	37.86069481943718	40.0	38.0	40.0	34.0	40.0
42	37.79693174267862	40.0	38.0	40.0	33.0	40.0
43	37.75721443210459	40.0	38.0	40.0	33.0	40.0
44	37.696806401222474	40.0	38.0	40.0	33.0	40.0
45	37.482113160240935	40.0	38.0	40.0	33.0	40.0
46	37.452715301840264	40.0	37.0	40.0	33.0	40.0
47	37.40727340599205	40.0	37.0	40.0	33.0	40.0
48	37.30476638625855	40.0	37.0	40.0	32.0	40.0
49	37.2353736783544	40.0	37.0	40.0	32.0	40.0
50	37.142727140759135	40.0	37.0	40.0	32.0	40.0
51	36.63664124825403	39.0	36.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	43.0
9	122.0
10	236.0
11	308.0
12	468.0
13	822.0
14	1181.0
15	1757.0
16	2747.0
17	4076.0
18	6369.0
19	9526.0
20	14444.0
21	21367.0
22	30407.0
23	41060.0
24	53964.0
25	69350.0
26	85930.0
27	105125.0
28	128017.0
29	155809.0
30	187145.0
31	225870.0
32	272129.0
33	333953.0
34	419763.0
35	561834.0
36	802980.0
37	1326278.0
38	2898948.0
39	1.2173909E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.19645773447776	28.659011949372648	24.749635607888507	31.39489470826109
2	24.120656049260177	25.003558898796957	30.32375896759732	20.552026084345552
3	17.409009315938018	25.18444826936194	31.778347870394086	25.62819454430596
4	18.17750071704663	27.670490815031464	30.919241237760424	23.232767230161482
5	23.484137343497487	28.11225574392221	28.48773336118253	19.915873551397773
6	22.986252668672492	26.37419390566044	29.138236858835164	21.501316566831907
7	23.247815428034453	27.17743661172369	28.743166487944237	20.83158147229762
8	22.20235191294196	25.479678863431687	28.93161005384145	23.386359169784903
9	22.292355184420177	24.928729226840648	30.068260631978163	22.710654956761008
10	22.331977089419702	26.064150868817737	28.756454046666068	22.847417995096496
11	22.209108553786923	27.537951304232326	27.599368015817866	22.65357212616289
12	22.249357467031153	26.3394074882441	28.496210512650972	22.91502453207378
13	22.57760380723419	26.537908266386406	26.807135574531664	24.077352351847733
14	22.825352320948767	27.682795224825597	26.691003615480017	22.80084883874562
15	22.54358484790937	27.64969922163698	26.747148441744063	23.059567488709586
16	22.89319461302606	26.464222257468446	26.45060363839341	24.191979491112082
17	24.429103977128346	26.15020148032132	26.465852478904683	22.95484206364565
18	22.925392740408252	26.15106424366604	26.38309742273528	24.54044559319043
19	23.125282968820837	27.35280830973525	26.332028855220386	23.189879866223528
20	24.359736800999922	26.184511371471686	26.353286942748934	23.10246488477946
21	23.140306086364014	27.291241116170983	26.488364582922642	23.080088214542357
22	24.285805004850033	26.086838535144214	26.408052350874616	23.219304109131137
23	23.106272078841318	26.215074261351685	27.78448592998515	22.894167729821845
24	23.078503137699737	26.88606337237538	26.44534178520383	23.59009170472105
25	22.933002112466017	26.171399375058375	26.3673519916942	24.528246520781412
26	23.057566078392483	26.88110248314326	27.071647780676727	22.989683657787527
27	23.020843459516485	25.944577888519138	27.837244911727772	23.1973337402366
28	23.877366818181954	26.031375893850413	26.791169436588447	23.300087851379185
29	24.044010561427196	26.081671987207827	26.81720783497464	23.057109616390335
30	23.854929955153363	25.970370499673407	26.857617262329512	23.31708228284372
31	23.611565483085776	26.169282595224242	26.708961131608426	23.510190790081552
32	23.5979869925384	26.42445488655615	26.717167415515153	23.260390705390293
33	23.400258688553567	26.25637654844702	26.783725594707285	23.559639168292122
34	23.493251535342548	26.29120811045698	26.63680000674159	23.578740347458876
35	23.537362819374174	26.347904703976365	26.714358418578865	23.400374058070593
36	23.365096066190404	26.26192431739619	26.75595163749975	23.61702797891366
37	23.532437042603753	26.283132244265158	26.527916262998758	23.65651445013233
38	23.665382854745467	26.387832588999306	26.666454988683252	23.280329567571975
39	23.471135700535246	26.284225746643923	26.71801011459604	23.52662843822479
40	23.53881246243594	26.33871527114194	26.536393414467195	23.586078851954927
41	23.598744418498008	26.388183713616343	26.72633176801979	23.28674009986586
42	23.42773168180365	26.244899789535904	26.82518337984731	23.502185148813133
43	23.504136398470663	26.250537847672312	26.63455782525852	23.61076792859851
44	23.610983619434688	26.242010535544296	26.697384051378158	23.44962179364286
45	23.631479264937667	26.006486174568526	26.892554161724586	23.469480398769218
46	23.499285862689607	26.196258997944515	26.65298183552099	23.651473303844885
47	23.593226745944587	26.215495610892127	26.694469717056762	23.496807926106527
48	23.464685039713697	26.094894337072212	26.787447515647866	23.652973107566226
49	23.579733528518492	26.250417462089327	26.49084753557168	23.6790014738205
50	23.638933138950748	26.335324410554566	26.64531728673769	23.380425163756996
51	23.551081759768362	26.15623580766838	26.69354676092055	23.599135671642703
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10890.0
1	7201.5
2	3513.0
3	2892.0
4	2271.0
5	2401.5
6	2532.0
7	2957.5
8	3383.0
9	4249.5
10	5116.0
11	7309.0
12	9502.0
13	13059.0
14	16616.0
15	22149.5
16	27683.0
17	38283.5
18	48884.0
19	66078.5
20	83273.0
21	107761.0
22	132249.0
23	202935.0
24	273621.0
25	277338.0
26	322090.5
27	363126.0
28	406531.5
29	449937.0
30	491040.0
31	532143.0
32	576715.5
33	621288.0
34	680415.0
35	739542.0
36	811326.5
37	883111.0
38	941485.0
39	999859.0
40	1064448.5
41	1129038.0
42	1184233.5
43	1239429.0
44	1284766.0
45	1330103.0
46	1362830.0
47	1395557.0
48	1406694.0
49	1417831.0
50	1424069.5
51	1430308.0
52	1403212.5
53	1376117.0
54	1315042.5
55	1253968.0
56	1161150.5
57	1068333.0
58	971425.0
59	874517.0
60	773774.5
61	673032.0
62	582877.5
63	492723.0
64	417302.0
65	341881.0
66	268907.0
67	195933.0
68	158435.5
69	120938.0
70	89693.5
71	58449.0
72	42022.5
73	25596.0
74	18828.0
75	8800.0
76	5540.0
77	4049.5
78	2559.0
79	1857.5
80	1156.0
81	966.0
82	776.0
83	534.5
84	293.0
85	207.5
86	122.0
87	87.0
88	52.0
89	35.0
90	18.0
91	14.5
92	11.0
93	6.5
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.9935942E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.06109248567418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.06019919481116	31.11928022996468
2	16.237876048807276	14.633928688124367
3	5.826520574253105	7.876481473983079
4	2.7208726181996776	4.904219707617366
5	1.4910798996366394	3.35948446305282
6	0.9296579938688364	2.513484290506198
7	0.614848635046059	1.9394025845950698
8	0.4476170565220523	1.613609086568436
9	0.3394090298717532	1.376472751697162
>10	2.08786203502815	18.217658401313322
>50	0.16808430273884933	5.124093650131163
>100	0.07269990408331084	5.684233294392255
>500	0.0025980291486235804	0.7887912650985365
>1k	6.282196764827432E-4	0.38079861757770217
>5k	3.4843731177374084E-5	0.11228284103000923
>10k+	1.1614577059124464E-5	0.355778654347855
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	67979	0.340987147735482	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2038558298373862E-4	0.0	0.0	0.0	0.0
2	1.5048197872967326E-4	0.0	0.0	0.0	5.016065957655776E-6
3	1.5549804468732904E-4	5.016065957655776E-6	0.0	0.0	5.016065957655776E-6
4	1.5549804468732904E-4	5.016065957655776E-6	5.016065957655776E-6	0.0	1.0032131915311551E-5
5	6.520885744952508E-4	5.016065957655776E-6	5.016065957655776E-6	0.0	1.5048197872967327E-5
6	6.520885744952508E-4	5.016065957655776E-6	5.016065957655776E-6	0.0	2.0064263830623103E-5
7	6.621207064105624E-4	5.016065957655776E-6	5.016065957655776E-6	0.0	2.0064263830623103E-5
8	6.671367723682181E-4	5.016065957655776E-6	5.016065957655776E-6	0.0	2.0064263830623103E-5
9	6.671367723682181E-4	5.016065957655776E-6	5.016065957655776E-6	0.0	2.508032978827888E-5
10	7.022492340718085E-4	5.016065957655776E-6	5.016065957655776E-6	0.0	2.508032978827888E-5
11	7.273295638600875E-4	5.016065957655776E-6	5.016065957655776E-6	0.0	3.511246170359043E-5
12	7.925384213096126E-4	5.016065957655776E-6	5.016065957655776E-6	0.0	4.514459361890198E-5
13	8.426990808861703E-4	5.016065957655776E-6	5.016065957655776E-6	0.0	5.016065957655776E-5
14	8.828276085474165E-4	5.016065957655776E-6	5.016065957655776E-6	0.0	5.5176725534213534E-5
15	9.480364659969416E-4	5.016065957655776E-6	5.016065957655776E-6	1.0032131915311551E-5	6.520885744952508E-5
16	0.001003213191531155	5.016065957655776E-6	5.016065957655776E-6	1.0032131915311551E-5	6.520885744952508E-5
17	0.0010433417191924013	5.016065957655776E-6	5.016065957655776E-6	1.0032131915311551E-5	8.025705532249241E-5
18	0.001093502378768959	5.016065957655776E-6	5.016065957655776E-6	1.0032131915311551E-5	8.527312128014819E-5
19	0.001138646972387861	5.016065957655776E-6	5.016065957655776E-6	1.0032131915311551E-5	9.530525319545974E-5
20	0.0011536951702608284	1.5048197872967327E-5	5.016065957655776E-6	1.0032131915311551E-5	1.1035345106842707E-4
21	0.001183791566006763	1.5048197872967327E-5	5.016065957655776E-6	1.0032131915311551E-5	1.1035345106842707E-4
22	0.0012339522255833209	1.5048197872967327E-5	5.016065957655776E-6	1.0032131915311551E-5	1.1536951702608283E-4
23	0.001254016489413944	1.5048197872967327E-5	5.016065957655776E-6	1.0032131915311551E-5	1.4044984681436173E-4
24	0.0013142092809058131	1.5048197872967327E-5	5.016065957655776E-6	1.0032131915311551E-5	1.454659127720175E-4
25	0.0013944663362283056	1.5048197872967327E-5	5.016065957655776E-6	1.0032131915311551E-5	1.5549804468732904E-4
26	0.0014345948638895519	1.5048197872967327E-5	5.016065957655776E-6	1.0032131915311551E-5	1.6051411064498482E-4
27	0.0014646912596354865	1.5048197872967327E-5	5.016065957655776E-6	1.5048197872967327E-5	1.6051411064498482E-4
28	0.0015098358532543885	1.5048197872967327E-5	5.016065957655776E-6	1.5048197872967327E-5	1.6051411064498482E-4
29	0.0015900929085768808	1.5048197872967327E-5	5.016065957655776E-6	1.5048197872967327E-5	1.7054624256029638E-4
30	0.0016753660298570291	1.5048197872967327E-5	5.016065957655776E-6	1.5048197872967327E-5	1.7556230851795214E-4
31	0.001720510623475931	1.5048197872967327E-5	5.016065957655776E-6	1.5048197872967327E-5	1.8057837447560792E-4
32	0.0018007676787984235	1.5048197872967327E-5	5.016065957655776E-6	1.5048197872967327E-5	2.0064263830623104E-4
33	0.0018910568660362274	1.5048197872967327E-5	5.016065957655776E-6	1.5048197872967327E-5	2.5581936384044455E-4
34	0.0019512496575280968	1.5048197872967327E-5	5.016065957655776E-6	2.0064263830623103E-5	2.5581936384044455E-4
35	0.0020415388447659008	1.5048197872967327E-5	5.016065957655776E-6	2.0064263830623103E-5	2.9594789150169074E-4
36	0.002141860163919016	1.5048197872967327E-5	5.016065957655776E-6	2.508032978827888E-5	2.9594789150169074E-4
37	0.002282310010733378	1.5048197872967327E-5	5.016065957655776E-6	3.0096395745934654E-5	2.9594789150169074E-4
38	0.0024026955937171165	1.5048197872967327E-5	5.016065957655776E-6	3.0096395745934654E-5	2.9594789150169074E-4
39	0.002482952649039609	1.5048197872967327E-5	5.016065957655776E-6	3.0096395745934654E-5	3.059800234170023E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	31405	0.0	35.87964	12
CAACGCA	31605	0.0	35.68818	13
AACGCAG	32205	0.0	34.98137	14
ACGCAGA	32260	0.0	34.886856	15
GGTATCA	33100	0.0	33.97432	8
ATCAACG	33290	0.0	33.868275	11
GTATCAA	33335	0.0	33.73481	9
AAGCAGT	33250	0.0	33.65188	1
GTGGTAT	33720	0.0	33.34297	6
CGCAGAG	33815	0.0	33.28922	16
TGGTATC	33835	0.0	33.229645	7
AGTGGTA	33930	0.0	33.090183	5
AGAGTAC	33195	0.0	32.43335	19
TATCAAC	34800	0.0	32.35345	10
CAGAGTA	35835	0.0	30.175806	18
CAGTGGT	37390	0.0	30.14242	4
GCAGAGT	37420	0.0	30.016035	17
GCAGTGG	38320	0.0	29.387396	3
AGCAGTG	38810	0.0	28.952589	2
GTACATG	24040	0.0	28.855032	22
>>END_MODULE