FastQCFastQC Report
Thu 26 May 2016
SRR805297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18385741
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9511785.173454798476711No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT468770.2549638875039086No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370570.2015529316985375No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC345300.1878085849245891No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG335720.18259802528492053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2335850.043.56381
GTGGTAT2352850.043.19076
TCAACGC2356300.043.11887412
AGTGGTA2351250.043.1100465
CAACGCA2358550.043.0729713
GTATCAA2359400.043.0345659
GGTATCA2360050.043.0265248
ACGCAGA2362950.042.98895315
AACGCAG2364750.042.96384414
TGGTATC2364250.042.9634177
ATCAACG2366500.042.90545311
CGCAGAG2376750.042.73745316
TATCAAC2379750.042.69492310
AGCAGTG2388300.042.6089672
AGAGTAC2311200.042.58372519
GCAGTGG2391850.042.5137443
CAGTGGT2392800.042.455494
CAGAGTA2331800.042.31173318
GCAGAGT2395650.042.20211417
GAGTACT1167100.042.1949720