##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805297_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18385741
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.45262505329538	37.0	37.0	37.0	35.0	37.0
2	36.460428328670574	37.0	37.0	37.0	35.0	37.0
3	38.279716003831446	39.0	39.0	39.0	37.0	39.0
4	38.24100850762556	39.0	39.0	39.0	37.0	39.0
5	38.20966296653477	39.0	39.0	39.0	37.0	39.0
6	38.21086134086192	39.0	39.0	39.0	37.0	39.0
7	38.11761679880077	39.0	39.0	39.0	37.0	39.0
8	39.12243982986598	40.0	40.0	40.0	37.0	40.0
9	39.125194410168184	40.0	40.0	40.0	37.0	40.0
10	38.837960623942216	40.0	39.0	40.0	36.0	40.0
11	39.000265096739916	40.0	39.0	40.0	37.0	40.0
12	39.07431286016702	40.0	40.0	40.0	37.0	40.0
13	39.12811885036344	40.0	40.0	40.0	37.0	40.0
14	39.097553642249174	40.0	40.0	40.0	37.0	40.0
15	39.076224341461135	40.0	40.0	40.0	37.0	40.0
16	39.02646670591085	40.0	40.0	40.0	37.0	40.0
17	38.97557444108453	40.0	40.0	40.0	37.0	40.0
18	38.944857212989135	40.0	40.0	40.0	37.0	40.0
19	38.89123109044123	40.0	39.0	40.0	37.0	40.0
20	38.822003257850746	40.0	39.0	40.0	36.0	40.0
21	38.68383819830814	40.0	39.0	40.0	36.0	40.0
22	38.74453926007116	40.0	39.0	40.0	36.0	40.0
23	38.27676926374629	40.0	38.0	40.0	35.0	40.0
24	38.10947744776781	40.0	38.0	40.0	35.0	40.0
25	38.49759860100281	40.0	39.0	40.0	36.0	40.0
26	38.59141450975514	40.0	39.0	40.0	36.0	40.0
27	38.64197428866207	40.0	39.0	40.0	36.0	40.0
28	38.646977785665534	40.0	39.0	40.0	36.0	40.0
29	38.66561973216092	40.0	39.0	40.0	36.0	40.0
30	38.66473306678257	40.0	39.0	40.0	36.0	40.0
31	38.613279116680694	40.0	39.0	40.0	36.0	40.0
32	38.59627719111239	40.0	39.0	40.0	36.0	40.0
33	38.51401425702668	40.0	39.0	40.0	35.0	40.0
34	38.50308453708774	40.0	39.0	40.0	35.0	40.0
35	38.46457784867088	40.0	39.0	40.0	35.0	40.0
36	38.35089741555698	40.0	39.0	40.0	35.0	40.0
37	38.20428396114141	40.0	38.0	40.0	35.0	40.0
38	38.03392471372244	40.0	38.0	40.0	35.0	40.0
39	37.98801973768694	40.0	38.0	40.0	35.0	40.0
40	37.91901642691475	40.0	38.0	40.0	34.0	40.0
41	37.84259367082349	40.0	37.0	40.0	34.0	40.0
42	37.79010625679977	40.0	37.0	40.0	34.0	40.0
43	37.751936895010104	40.0	37.0	40.0	34.0	40.0
44	37.688119940338545	40.0	37.0	40.0	34.0	40.0
45	37.531342848787006	40.0	37.0	40.0	33.0	40.0
46	37.48209403145623	40.0	36.0	40.0	33.0	40.0
47	37.413949538395	40.0	36.0	40.0	33.0	40.0
48	37.30531339476609	40.0	36.0	40.0	33.0	40.0
49	37.1732431126926	40.0	36.0	40.0	33.0	40.0
50	37.021210023572074	40.0	35.0	40.0	33.0	40.0
51	36.45843471851366	39.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	8.0
8	62.0
9	149.0
10	196.0
11	215.0
12	216.0
13	223.0
14	291.0
15	388.0
16	721.0
17	1267.0
18	2177.0
19	3772.0
20	6881.0
21	10759.0
22	16132.0
23	22605.0
24	31444.0
25	41653.0
26	53905.0
27	68533.0
28	85721.0
29	107283.0
30	131437.0
31	161828.0
32	203106.0
33	257417.0
34	344161.0
35	495643.0
36	784832.0
37	1584880.0
38	3003217.0
39	1.0964619E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.716909261367274	40.249506397376095	22.468819722849354	24.564764618407274
2	23.9798602623631	40.25005029712971	19.113600044730315	16.65648939577687
3	34.02574854067617	24.547887409052482	20.794663647225313	20.631700403046036
4	19.769021003831174	23.291196150321056	22.186628213679285	34.75315463216848
5	20.292943319499607	36.031726977988	23.55253454293738	20.12279515957502
6	33.000715065006084	23.273851187178153	22.528577988779457	21.19685575903631
7	21.352721111430863	23.0952617030774	35.17192480846978	20.380092377021956
8	32.13633325956239	23.70820409142063	23.76312708854106	20.392335560475914
9	32.3528651904756	23.222104564618853	24.132755922102895	20.292274322802655
10	20.24646164655534	23.838876007227558	35.58774160910893	20.326920737108175
11	20.102257504878374	36.145075686642166	23.539274266944147	20.213392541535313
12	20.030756443267638	23.745450346548445	35.657121461680546	20.566671748503364
13	20.217955860468177	23.910986236562344	23.237529561631483	32.633528341338
14	20.49321808677714	36.21950292892737	22.99036519659447	20.296913787701023
15	20.338810385722283	36.046705977202656	22.971290632235057	20.64319300484
16	20.67835612391146	23.580654160199472	23.014476272672393	32.726513443216675
17	33.06098459670459	23.423521521378987	23.00018258714729	20.515311294769138
18	20.671742302907454	23.491362137647865	22.915927076314194	32.92096848313049
19	20.708901534074695	35.810941751001494	22.94776696789104	20.53238974703277
20	33.03895121768549	23.733533502946656	22.94418810751223	20.283327171855625
21	20.8047312316648	35.698697158847175	23.009282030025332	20.487289579462693
22	32.75892442953482	23.4429115475955	23.275695007342918	20.522469015526763
23	20.6638883904652	23.58838841469593	35.438201810848966	20.30952138398991
24	20.692715077406998	35.02440831729328	23.11525001902289	21.167626586276832
25	20.385612959521186	23.59400146015328	23.161677301991798	32.85870827833374
26	20.609732291997368	29.527626871280304	29.413348094047446	20.449292742674878
27	20.77583383775503	23.36210980019788	35.36216462529305	20.49989173675404
28	26.7044009811734	23.38476866393364	29.300244140282405	20.610586214610553
29	26.851487791544542	23.477987642706378	29.189201566583584	20.481322999165492
30	26.771142919939965	23.35079668532261	29.176251313449917	20.701809081287504
31	24.671363531119034	24.250831119616013	29.64950936706875	21.428295982196204
32	23.441176507381453	25.234919821833667	29.899931691629945	21.42397197915493
33	23.062518937909545	24.74067267672268	30.245933519894574	21.950874865473192
34	22.440792568545376	24.56371489188279	30.56967897024112	22.42581356933071
35	22.21565070453239	24.85534850077568	30.794505372397012	22.13449542229492
36	22.501442830071415	24.790586357112286	30.71882172168095	21.989149091135353
37	22.815398084852824	24.680381389034032	30.570108651046485	21.934111875066662
38	22.857718924681905	24.806549814881	30.66306655793748	21.672664702499617
39	22.898304724296942	24.700652532851407	30.605908132829672	21.795134610021975
40	22.367540149727986	25.171452159583886	30.394282177694116	22.066725512994008
41	22.65093911635109	25.37386445289314	30.303886038642663	21.671310392113107
42	22.4913262946541	25.348448017406533	30.358749206790197	21.80147648114917
43	22.398275924804988	25.032599991482527	30.881469504003128	21.68765457970935
44	22.685155849851252	24.82727783449141	30.512362814204767	21.97520350145257
45	22.8620320497281	24.49499315801305	30.482274279834577	22.160700512424274
46	22.438535384567857	24.712460596502474	30.267787411994977	22.58121660693469
47	22.4097902825891	24.800730087517277	30.45805986280346	22.33141976709016
48	22.26809895777385	24.73255769239869	30.382408846072618	22.61693450375484
49	21.993957164957344	25.441868239088105	30.113580953848963	22.450593642105588
50	22.691068040173086	25.227120299366774	30.111748011679268	21.97006364878087
51	22.622634573172764	24.958439260076602	30.186566861787078	22.23235930496356
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	38853.0
1	22765.5
2	6678.0
3	5430.0
4	4182.0
5	4158.0
6	4134.0
7	4571.0
8	5008.0
9	5689.0
10	6370.0
11	8701.0
12	11032.0
13	14208.5
14	17385.0
15	21906.0
16	26427.0
17	36243.5
18	46060.0
19	59058.0
20	72056.0
21	95401.5
22	118747.0
23	641961.5
24	1165176.0
25	733681.0
26	305508.5
27	308831.0
28	335306.5
29	361782.0
30	384302.5
31	406823.0
32	435288.0
33	463753.0
34	507131.5
35	550510.0
36	605998.5
37	661487.0
38	710218.5
39	758950.0
40	819061.5
41	879173.0
42	935917.0
43	992661.0
44	1048773.5
45	1104886.0
46	1169185.5
47	1233485.0
48	1247544.5
49	1261604.0
50	1279245.5
51	1296887.0
52	1288632.0
53	1280377.0
54	1252223.0
55	1224069.0
56	1144237.5
57	1064406.0
58	947791.0
59	831176.0
60	731901.0
61	632626.0
62	551898.5
63	471171.0
64	402519.0
65	333867.0
66	263420.5
67	192974.0
68	158245.5
69	123517.0
70	93034.5
71	62552.0
72	46509.0
73	30466.0
74	22913.0
75	11685.5
76	8011.0
77	6038.5
78	4066.0
79	3310.5
80	2555.0
81	2263.0
82	1971.0
83	1400.5
84	830.0
85	571.0
86	312.0
87	233.0
88	154.0
89	113.5
90	73.0
91	57.5
92	42.0
93	31.0
94	20.0
95	17.5
96	15.0
97	9.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.8385741E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.87369548825569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.23829681278443	17.501395577368484
2	21.103893777258403	13.87525955300174
3	10.010348396359236	9.872314347397865
4	5.301401290906003	6.971066067931582
5	2.9245168271058253	4.806983781227831
6	1.7218406799135229	3.3961959714460974
7	1.0718383775597053	2.466470190556665
8	0.7255079094839095	1.908010087255601
9	0.5238491816456563	1.5498772631272122
>10	3.099753624118292	19.873457785245467
>50	0.1907775523989666	4.180161623445777
>100	0.08059853403251033	4.843415229704609
>500	0.005882777034489795	1.2997632353277726
>1k	0.0012882054453311638	0.8217411062155191
>5k	3.434232562876102E-5	0.06854475510058558
>10k+	1.71711628143805E-4	6.5653434256472
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	951178	5.173454798476711	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT	46877	0.2549638875039086	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37057	0.2015529316985375	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC	34530	0.1878085849245891	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG	33572	0.18259802528492053	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.8950977825696556E-5	0.0	0.0	0.0	0.0
2	8.15849630428276E-5	0.0	0.0	0.0	0.0
3	8.15849630428276E-5	0.0	0.0	0.0	0.0
4	8.15849630428276E-5	0.0	0.0	0.0	0.0
5	8.702396057901609E-5	1.0877995072377012E-5	0.0	0.0	5.438997536188506E-6
6	1.3597493840471266E-4	1.0877995072377012E-5	0.0	0.0	5.438997536188506E-6
7	1.3597493840471266E-4	1.0877995072377012E-5	0.0	0.0	5.438997536188506E-6
8	1.3597493840471266E-4	1.0877995072377012E-5	0.0	0.0	5.438997536188506E-6
9	1.3597493840471266E-4	1.0877995072377012E-5	0.0	0.0	5.438997536188506E-6
10	1.4141393594090115E-4	1.0877995072377012E-5	0.0	0.0	5.438997536188506E-6
11	1.4141393594090115E-4	1.0877995072377012E-5	0.0	0.0	5.438997536188506E-6
12	1.4685293347708968E-4	2.1755990144754023E-5	0.0	0.0	1.0877995072377012E-5
13	1.4685293347708968E-4	2.1755990144754023E-5	0.0	0.0	2.1755990144754023E-5
14	1.5229193101327817E-4	2.1755990144754023E-5	0.0	0.0	2.1755990144754023E-5
15	1.903649137665977E-4	2.1755990144754023E-5	0.0	0.0	2.1755990144754023E-5
16	2.0124290883897472E-4	2.1755990144754023E-5	0.0	5.438997536188506E-6	2.719498768094253E-5
17	2.3387689405610576E-4	2.1755990144754023E-5	0.0	5.438997536188506E-6	2.719498768094253E-5
18	2.719498768094253E-4	2.1755990144754023E-5	0.0	5.438997536188506E-6	2.719498768094253E-5
19	2.9370586695417935E-4	2.1755990144754023E-5	0.0	5.438997536188506E-6	3.263398521713103E-5
20	3.1002285956274484E-4	3.263398521713103E-5	0.0	5.438997536188506E-6	3.807298275331954E-5
21	3.263398521713104E-4	3.263398521713103E-5	0.0	5.438997536188506E-6	4.3511980289508046E-5
22	3.589738373884414E-4	3.263398521713103E-5	0.0	5.438997536188506E-6	4.8950977825696556E-5
23	3.807298275331954E-4	3.263398521713103E-5	0.0	5.438997536188506E-6	7.070696797045058E-5
24	4.0792481521413794E-4	3.263398521713103E-5	0.0	5.438997536188506E-6	7.070696797045058E-5
25	4.7863178318458856E-4	3.263398521713103E-5	0.0	5.438997536188506E-6	7.070696797045058E-5
26	5.384607560826621E-4	3.263398521713103E-5	0.0	5.438997536188506E-6	7.070696797045058E-5
27	5.928507314445472E-4	3.263398521713103E-5	0.0	5.438997536188506E-6	7.614596550663909E-5
28	6.472407068064323E-4	3.263398521713103E-5	0.0	5.438997536188506E-6	7.614596550663909E-5
29	6.907526870959402E-4	3.263398521713103E-5	0.0	5.438997536188506E-6	8.702396057901609E-5
30	7.777766476749564E-4	3.263398521713103E-5	0.0	5.438997536188506E-6	8.702396057901609E-5
31	9.300685786882345E-4	3.263398521713103E-5	0.0	5.438997536188506E-6	8.702396057901609E-5
32	0.0011530674776719632	3.263398521713103E-5	0.0	5.438997536188506E-6	9.24629581152046E-5
33	0.001278164421004299	3.263398521713103E-5	0.0	5.438997536188506E-6	1.0334095318758161E-4
34	0.0013651883815833151	3.263398521713103E-5	0.0	5.438997536188506E-6	1.0334095318758161E-4
35	0.0015174803125965932	3.263398521713103E-5	0.0	5.438997536188506E-6	1.0334095318758161E-4
36	0.0015664312904222898	3.263398521713103E-5	0.0	5.438997536188506E-6	1.0877995072377012E-4
37	0.0017567962041888875	3.263398521713103E-5	0.0	1.6316992608565517E-5	1.1965794579614714E-4
38	0.0019090881352021656	3.263398521713103E-5	0.0	1.6316992608565517E-5	1.1965794579614714E-4
39	0.0020722580612878207	3.263398521713103E-5	0.0	1.6316992608565517E-5	1.2509694333233564E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	233585	0.0	43.5638	1
GTGGTAT	235285	0.0	43.1907	6
TCAACGC	235630	0.0	43.118874	12
AGTGGTA	235125	0.0	43.110046	5
CAACGCA	235855	0.0	43.07297	13
GTATCAA	235940	0.0	43.034565	9
GGTATCA	236005	0.0	43.026524	8
ACGCAGA	236295	0.0	42.988953	15
AACGCAG	236475	0.0	42.963844	14
TGGTATC	236425	0.0	42.963417	7
ATCAACG	236650	0.0	42.905453	11
CGCAGAG	237675	0.0	42.737453	16
TATCAAC	237975	0.0	42.694923	10
AGCAGTG	238830	0.0	42.608967	2
AGAGTAC	231120	0.0	42.583725	19
GCAGTGG	239185	0.0	42.513744	3
CAGTGGT	239280	0.0	42.45549	4
CAGAGTA	233180	0.0	42.311733	18
GCAGAGT	239565	0.0	42.202114	17
GAGTACT	116710	0.0	42.19497	20
>>END_MODULE