FastQCFastQC Report
Thu 26 May 2016
SRR805296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27886171
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14247285.109084355826406No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT768280.27550573364840947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG512730.18386532880401543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC4098900.043.87085712
CAACGCA4087950.043.82435213
AAGCAGT4074450.043.808861
GTATCAA4114250.043.6989759
ACGCAGA4104800.043.68556615
AACGCAG4104750.043.6789714
GGTATCA4114950.043.6784178
ATCAACG4115450.043.6638211
AGTGGTA4111400.043.63245
TGGTATC4105500.043.6276977
CGCAGAG4070500.043.5766516
GTGGTAT4113900.043.5364276
TATCAAC4140850.043.41554610
AGAGTAC4043900.043.3035619
GCAGTGG4141600.043.145833
CAGTGGT4137750.043.1261564
AGCAGTG4143150.043.063432
GCAGAGT4102150.043.0188517
CAGAGTA4093900.042.95989218
GAGTACT1869200.042.81524720
Produced by FastQC (version 0.11.5)