##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805296_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27886171
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.57086991254554	37.0	37.0	37.0	35.0	37.0
2	36.50004064738755	37.0	37.0	37.0	35.0	37.0
3	38.3150324940631	39.0	39.0	39.0	37.0	39.0
4	38.12501361337847	39.0	39.0	39.0	37.0	39.0
5	38.19373122254755	39.0	39.0	39.0	37.0	39.0
6	38.17069589797753	39.0	39.0	39.0	37.0	39.0
7	38.01543725741336	39.0	39.0	39.0	37.0	39.0
8	39.08434252232047	40.0	40.0	40.0	37.0	40.0
9	39.14196685518424	40.0	40.0	40.0	38.0	40.0
10	38.839639224761264	40.0	39.0	40.0	36.0	40.0
11	39.04778228606573	40.0	39.0	40.0	37.0	40.0
12	38.9652953429856	40.0	40.0	40.0	37.0	40.0
13	39.09528805514389	40.0	40.0	40.0	37.0	40.0
14	39.135546827135215	40.0	40.0	40.0	37.0	40.0
15	39.13620744131563	40.0	40.0	40.0	38.0	40.0
16	39.113672830880944	40.0	40.0	40.0	37.0	40.0
17	39.01429870024106	40.0	40.0	40.0	37.0	40.0
18	39.01151900703758	40.0	40.0	40.0	37.0	40.0
19	38.826039078653	40.0	40.0	40.0	37.0	40.0
20	38.87757311679685	40.0	40.0	40.0	37.0	40.0
21	38.78622859337698	40.0	39.0	40.0	36.0	40.0
22	38.16934042325137	40.0	39.0	40.0	35.0	40.0
23	38.103992512991475	40.0	38.0	40.0	34.0	40.0
24	38.50071947848272	40.0	38.0	40.0	36.0	40.0
25	38.659279110064986	40.0	39.0	40.0	36.0	40.0
26	38.555583590160154	40.0	39.0	40.0	36.0	40.0
27	38.673067521532445	40.0	39.0	40.0	36.0	40.0
28	38.70348858579401	40.0	39.0	40.0	36.0	40.0
29	38.66184884256788	40.0	39.0	40.0	36.0	40.0
30	38.629191149978965	40.0	39.0	40.0	36.0	40.0
31	38.53655275943047	40.0	39.0	40.0	36.0	40.0
32	38.463503146416194	40.0	39.0	40.0	35.0	40.0
33	38.373037409832996	40.0	39.0	40.0	35.0	40.0
34	38.34653879157522	40.0	38.0	40.0	35.0	40.0
35	38.28609219960675	40.0	38.0	40.0	35.0	40.0
36	38.156446397750344	40.0	38.0	40.0	35.0	40.0
37	37.78044099349459	40.0	37.0	40.0	34.0	40.0
38	37.623679134722366	40.0	37.0	40.0	33.0	40.0
39	37.55091754977763	40.0	37.0	40.0	33.0	40.0
40	37.54840709396783	40.0	37.0	40.0	33.0	40.0
41	37.53347589384	40.0	37.0	40.0	33.0	40.0
42	37.47138300199048	40.0	37.0	40.0	33.0	40.0
43	37.395000159756606	40.0	36.0	40.0	33.0	40.0
44	37.18230129191993	40.0	36.0	40.0	33.0	40.0
45	37.44041381658314	40.0	37.0	40.0	33.0	40.0
46	37.46037718839205	40.0	37.0	40.0	33.0	40.0
47	37.431051111319654	40.0	37.0	40.0	33.0	40.0
48	37.26922936103347	40.0	36.0	40.0	33.0	40.0
49	37.206155803892905	40.0	36.0	40.0	33.0	40.0
50	37.101018458217155	40.0	36.0	40.0	33.0	40.0
51	36.413839569441066	39.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	100.0
9	277.0
10	320.0
11	388.0
12	337.0
13	389.0
14	457.0
15	546.0
16	846.0
17	1525.0
18	2846.0
19	5494.0
20	9750.0
21	16310.0
22	24509.0
23	35368.0
24	48563.0
25	66043.0
26	86531.0
27	108963.0
28	135358.0
29	166574.0
30	202674.0
31	247269.0
32	304940.0
33	389621.0
34	522558.0
35	756136.0
36	1234917.0
37	2530686.0
38	5395852.0
39	1.5590018E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.007258759189277	41.98785125430092	26.574537608623285	20.430352377886514
2	24.08346416580462	47.35798973620294	13.046133153239289	15.512412944753153
3	37.225913876810125	25.507349144491727	18.51902148918186	18.747715489516292
4	20.009685087278566	22.900383132556996	20.496750880570875	36.59318089959356
5	18.029395287004444	38.30956211234594	23.567204690812517	20.093837909837102
6	34.07626310546543	23.323876196556352	21.95942569526666	20.64043500271156
7	20.44616308205239	22.736861220566997	37.038806797821046	19.77816889955957
8	33.316076273074565	24.573219464228345	23.090079308485915	19.020624954211172
9	33.67033430297763	23.896805337670777	23.284222132898776	19.148638226452817
10	19.046354553301708	24.326183038897668	37.63030069635591	18.997161711444715
11	18.869399459682004	38.709175956785174	23.389887410501785	19.031537173031037
12	18.988110630175793	23.82824447286076	38.005282259798236	19.17836263716521
13	18.771211006344327	24.135766075593526	23.703802863433634	33.38922005462851
14	19.111515883625614	38.804861377347216	23.147577342188715	18.936045396838455
15	18.952716742646384	38.44117932146368	23.269992857750175	19.33611107813977
16	19.215216029479272	23.731422288129842	23.486211140281682	33.567150542109204
17	33.73601919030045	23.717720155986992	23.401233536149512	19.14502711756304
18	19.259126683258167	23.72600741779859	23.381826067121224	33.63303983182202
19	19.594927535946045	37.91555678260741	23.459520491357527	19.02999519008902
20	33.78701937960575	24.069059176320764	23.205921673506197	18.93799977056728
21	19.410033023178404	38.152738861136584	23.316047943620514	19.1211801720645
22	33.56564083322877	23.251532094528145	23.856520136809028	19.326306935434054
23	19.274299078206184	23.853784013588673	37.72696509678578	19.144951811419357
24	19.31749611662354	37.85174379085605	23.378591488949844	19.452168603570566
25	19.090293895135336	23.826071352714575	23.58225157552107	33.50138317662902
26	19.46145994729789	31.498135760553144	29.994874520420893	19.045529771728074
27	19.487820683592595	23.5888892741854	37.68622805906196	19.23706198316004
28	27.03232724205844	23.667523232214275	30.04891205752127	19.251237468206014
29	27.166996142998624	23.92519933984483	29.749609582470104	19.158194934686442
30	27.11626131819962	23.722392722901972	29.85893617305868	19.30240978583973
31	23.821531468052747	25.43076638237641	30.576159057476914	20.171543092093927
32	22.284669343812027	25.95697702635475	31.391261998644417	20.367091631188806
33	22.225457198838807	25.367190067076617	31.502284770469206	20.90506796361537
34	21.38842223982633	25.414496669334774	31.716247454697168	21.48083363614173
35	21.120422018497987	25.575009921584428	32.03759311380541	21.266974946112178
36	21.429718694617485	25.53366684870433	32.03234678579573	21.00426767088246
37	22.15480210603313	25.30541751321829	31.80549240697118	20.734287973777395
38	21.895160866653224	25.666488956120936	31.773888928673642	20.664461248552193
39	21.866408263794984	25.381752840861516	31.96992516469902	20.781913730644483
40	21.244451237138293	26.106811150229266	31.669640123773178	20.979097488859264
41	21.667223513762433	26.208603540443036	31.422765068750387	20.701407877044144
42	21.527168430545736	26.1238769567898	31.614498096565498	20.734456516098966
43	21.316221578071797	25.9624779608502	32.08706566419606	20.63423479688194
44	21.864586572319304	25.7004914729957	31.701709782960165	20.73321217172483
45	21.688201653787466	25.347990586445157	31.903996428910947	21.05981133085643
46	21.363757684767837	25.553307408177332	31.582166658879057	21.500768248175774
47	21.412193879181192	25.68346152650359	31.683123509498667	21.22122108481656
48	21.29417480800788	25.520875562299324	31.664655574262955	21.52029405542984
49	21.02820426655205	26.18932516766106	31.371911905725604	21.41055866006129
50	21.73851332977912	26.06969956542259	31.06934616444832	21.122440940349968
51	21.518841722658877	26.11348112295517	31.33547090419836	21.032206250187592
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16052.0
1	9687.0
2	3322.0
3	2723.0
4	2124.0
5	2301.5
6	2479.0
7	2898.0
8	3317.0
9	4251.0
10	5185.0
11	7803.0
12	10421.0
13	14527.0
14	18633.0
15	26122.5
16	33612.0
17	49424.0
18	65236.0
19	87797.0
20	110358.0
21	158767.5
22	207177.0
23	1004028.0
24	1800879.0
25	1180748.0
26	578613.5
27	596610.0
28	653479.5
29	710349.0
30	754917.0
31	799485.0
32	855673.5
33	911862.0
34	996425.5
35	1080989.0
36	1201413.5
37	1321838.0
38	1402599.0
39	1483360.0
40	1562183.5
41	1641007.0
42	1688071.0
43	1735135.0
44	1764335.0
45	1793535.0
46	1816804.0
47	1840073.0
48	1837014.0
49	1833955.0
50	1792689.0
51	1751423.0
52	1693462.5
53	1635502.0
54	1588982.5
55	1542463.0
56	1422780.5
57	1303098.0
58	1141554.5
59	980011.0
60	853272.5
61	726534.0
62	624710.0
63	522886.0
64	440056.5
65	357227.0
66	283655.0
67	210083.0
68	169980.5
69	129878.0
70	99091.5
71	68305.0
72	51719.0
73	35133.0
74	26536.5
75	13465.0
76	8990.0
77	6605.0
78	4220.0
79	3240.0
80	2260.0
81	1829.5
82	1399.0
83	1034.5
84	670.0
85	476.0
86	282.0
87	202.0
88	122.0
89	91.5
90	61.0
91	43.0
92	25.0
93	27.0
94	29.0
95	20.5
96	12.0
97	9.0
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.7886171E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.734707328733585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.64126610648569	20.71621922923898
2	20.075040426612553	13.946013076617641
3	8.653671361987076	9.017482262329931
4	4.139756803996287	5.751729639957782
5	2.174980028191956	3.7773647362544156
6	1.2012552219779593	2.5035149137510384
7	0.7611271547670326	1.850627027254921
8	0.5034604215169061	1.3990040314392458
9	0.36814109117525295	1.1508545751887733
>10	2.1362347274598252	14.68792666346223
>50	0.1997283193233596	4.541117996991624
>100	0.1299401646356525	9.798979601595766
>500	0.013488655311307956	3.0150951097622727
>1k	0.0015852009962397484	0.9065809257265331
>5k	1.6215778150327652E-4	0.3705548677323156
>10k+	1.6215778149999418E-4	6.5669353426963974
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1424728	5.109084355826406	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT	76828	0.27550573364840947	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG	51273	0.18386532880401543	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0399419841469092E-4	0.0	0.0	7.1720136837717875E-6	7.1720136837717875E-6
2	1.4344027367543574E-4	0.0	0.0	1.7930034209429468E-5	1.0758020525657682E-5
3	1.5419829420109345E-4	0.0	0.0	3.9446075260744835E-5	1.7930034209429468E-5
4	1.5778430104297934E-4	3.5860068418858938E-6	0.0	4.303208210263073E-5	2.5102047893201257E-5
5	1.757143352524088E-4	3.5860068418858938E-6	0.0	6.813412999583198E-5	3.5860068418858936E-5
6	1.757143352524088E-4	3.5860068418858938E-6	0.0	6.813412999583198E-5	5.379010262828841E-5
7	1.828863489361806E-4	3.5860068418858938E-6	0.0	7.530614367960378E-5	6.09621163120602E-5
8	1.9005836261995238E-4	3.5860068418858938E-6	0.0	8.606416420526146E-5	6.813412999583198E-5
9	2.0440238998749595E-4	3.5860068418858938E-6	0.0	8.965017104714735E-5	6.813412999583198E-5
10	2.0440238998749595E-4	3.5860068418858938E-6	0.0	9.323617788903324E-5	7.889215052148967E-5
11	2.1157440367126774E-4	7.1720136837717875E-6	0.0	1.3626825999166396E-4	8.606416420526146E-5
12	2.1874641735503952E-4	7.1720136837717875E-6	0.0	1.4702628051732166E-4	8.606416420526146E-5
13	2.330904447225831E-4	1.0758020525657682E-5	0.0	1.5061228735920756E-4	8.965017104714735E-5
14	2.4026245840635488E-4	1.0758020525657682E-5	0.0	1.5778430104297934E-4	8.965017104714735E-5
15	2.438484652482408E-4	1.0758020525657682E-5	0.0	1.5778430104297934E-4	8.965017104714735E-5
16	2.5819249261578434E-4	1.0758020525657682E-5	0.0	1.6495631472675113E-4	1.0040819157280503E-4
17	2.797085336670997E-4	1.0758020525657682E-5	0.0	1.757143352524088E-4	1.1116621209846271E-4
18	3.1198259524407276E-4	1.0758020525657682E-5	0.0	1.8647235577806649E-4	1.219242326241204E-4
19	3.2632662261161634E-4	1.0758020525657682E-5	0.0	1.9005836261995238E-4	1.219242326241204E-4
20	3.406706499791599E-4	1.0758020525657682E-5	0.0	1.9723037630372416E-4	1.2551023946600628E-4
21	3.8011672523990476E-4	1.0758020525657682E-5	0.0	2.0798839682938184E-4	1.3268225314977806E-4
22	4.2314880734253547E-4	1.0758020525657682E-5	0.0	2.0798839682938184E-4	1.3626825999166396E-4
23	4.661808894451662E-4	1.0758020525657682E-5	0.0	2.0798839682938184E-4	1.6137030788486523E-4
24	5.235569989153405E-4	1.0758020525657682E-5	0.0	2.259184310388113E-4	1.6137030788486523E-4
25	5.73761094701743E-4	1.0758020525657682E-5	0.0	2.295044378806972E-4	1.6854232156863702E-4
26	6.167931768043738E-4	1.0758020525657682E-5	0.0	2.295044378806972E-4	1.7212832841052291E-4
27	6.669972725907762E-4	1.0758020525657682E-5	0.0	2.330904447225831E-4	1.9364436946183827E-4
28	7.351314025866082E-4	1.0758020525657682E-5	0.0	2.438484652482408E-4	1.9364436946183827E-4
29	7.960935188986685E-4	1.0758020525657682E-5	0.0	2.5819249261578434E-4	1.9364436946183827E-4
30	8.857436899458158E-4	1.0758020525657682E-5	0.0	2.725365199833279E-4	1.9364436946183827E-4
31	0.001014839936253708	1.0758020525657682E-5	0.0	2.725365199833279E-4	2.0081638314561006E-4
32	0.001312478504130237	1.0758020525657682E-5	0.0	2.832945405089856E-4	2.0440238998749595E-4
33	0.0014344027367543576	1.0758020525657682E-5	0.0	2.940525610346433E-4	2.1874641735503952E-4
34	0.0014953648530664177	1.0758020525657682E-5	0.0	3.04810581560301E-4	2.259184310388113E-4
35	0.001585015024113565	1.0758020525657682E-5	0.0	3.2991262945350226E-4	2.4026245840635488E-4
36	0.001689009222528256	1.0758020525657682E-5	0.0	3.334986362953881E-4	2.546064857738985E-4
37	0.0018216914756780341	1.0758020525657682E-5	0.0	3.334986362953881E-4	2.5819249261578434E-4
38	0.001922099667250839	1.0758020525657682E-5	0.0	3.3708464313727404E-4	2.5819249261578434E-4
39	0.0020404378930330737	1.0758020525657682E-5	0.0	3.550146773467035E-4	2.6536450629955613E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	409890	0.0	43.870857	12
CAACGCA	408795	0.0	43.824352	13
AAGCAGT	407445	0.0	43.80886	1
GTATCAA	411425	0.0	43.698975	9
ACGCAGA	410480	0.0	43.685566	15
AACGCAG	410475	0.0	43.67897	14
GGTATCA	411495	0.0	43.678417	8
ATCAACG	411545	0.0	43.66382	11
AGTGGTA	411140	0.0	43.6324	5
TGGTATC	410550	0.0	43.627697	7
CGCAGAG	407050	0.0	43.57665	16
GTGGTAT	411390	0.0	43.536427	6
TATCAAC	414085	0.0	43.415546	10
AGAGTAC	404390	0.0	43.30356	19
GCAGTGG	414160	0.0	43.14583	3
CAGTGGT	413775	0.0	43.126156	4
AGCAGTG	414315	0.0	43.06343	2
GCAGAGT	410215	0.0	43.01885	17
CAGAGTA	409390	0.0	42.959892	18
GAGTACT	186920	0.0	42.815247	20
>>END_MODULE