FastQCFastQC Report
Thu 26 May 2016
SRR805295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18276975
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8587114.6983212484560495No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA471590.2580240986268242No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT283530.15512960979593177No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG194690.10652200377797748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2000600.043.2815131
TCAACGC2012850.043.2383212
CAACGCA2008200.043.20958713
AGTGGTA2019400.043.052395
AACGCAG2017650.043.0373214
GGTATCA2023800.043.033288
ACGCAGA2017900.043.00299515
GTGGTAT2016850.042.9908036
ATCAACG2023900.042.9822411
GTATCAA2027400.042.979069
TGGTATC2018450.042.9712147
CGCAGAG2002200.042.8266416
TATCAAC2041850.042.66277710
AGAGTAC1993700.042.5803819
CAGTGGT2041800.042.3938454
GCAGTGG2047650.042.3001983
GAGTACT1102600.042.222720
CAGAGTA2020400.042.20364818
GCAGAGT2026450.042.1864617
AGCAGTG2052550.042.1279642