##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805295_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18276975
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.037701151311964	35.0	35.0	35.0	33.0	37.0
2	35.92172342523858	37.0	35.0	37.0	35.0	37.0
3	38.24797238054985	39.0	39.0	39.0	37.0	39.0
4	38.11635831421775	39.0	39.0	39.0	37.0	39.0
5	38.201728021185126	39.0	39.0	39.0	37.0	39.0
6	38.16930389191866	39.0	39.0	39.0	37.0	39.0
7	38.04202593700544	39.0	39.0	39.0	37.0	39.0
8	39.105983293187194	40.0	40.0	40.0	37.0	40.0
9	39.15243064019073	40.0	40.0	40.0	38.0	40.0
10	38.92633020508043	40.0	39.0	40.0	37.0	40.0
11	39.0846823393915	40.0	39.0	40.0	37.0	40.0
12	38.97191220100701	40.0	40.0	40.0	37.0	40.0
13	39.08828085610447	40.0	40.0	40.0	37.0	40.0
14	39.11773562091101	40.0	40.0	40.0	37.0	40.0
15	39.11616588631325	40.0	40.0	40.0	37.0	40.0
16	39.09517297036298	40.0	40.0	40.0	37.0	40.0
17	38.991746281865574	40.0	40.0	40.0	37.0	40.0
18	38.97592079652131	40.0	40.0	40.0	37.0	40.0
19	38.793695893330266	40.0	39.0	40.0	36.0	40.0
20	38.831675482403405	40.0	39.0	40.0	36.0	40.0
21	38.7526792042994	40.0	39.0	40.0	36.0	40.0
22	38.104541643242385	40.0	39.0	40.0	35.0	40.0
23	38.13385913150289	40.0	38.0	40.0	34.0	40.0
24	38.52764699847759	40.0	39.0	40.0	36.0	40.0
25	38.66670808489917	40.0	39.0	40.0	36.0	40.0
26	38.579434178796	40.0	39.0	40.0	36.0	40.0
27	38.67021791078666	40.0	39.0	40.0	36.0	40.0
28	38.69874817906136	40.0	39.0	40.0	36.0	40.0
29	38.66216608601806	40.0	39.0	40.0	36.0	40.0
30	38.63522535868217	40.0	39.0	40.0	36.0	40.0
31	38.562696835772876	40.0	39.0	40.0	35.0	40.0
32	38.502290942565715	40.0	39.0	40.0	35.0	40.0
33	38.42938850657726	40.0	39.0	40.0	35.0	40.0
34	38.394942270260806	40.0	39.0	40.0	35.0	40.0
35	38.33131078857415	40.0	38.0	40.0	35.0	40.0
36	38.19596240625158	40.0	38.0	40.0	35.0	40.0
37	37.73080271762696	40.0	38.0	40.0	34.0	40.0
38	37.693527840356516	40.0	37.0	40.0	34.0	40.0
39	37.61900921788206	40.0	37.0	40.0	33.0	40.0
40	37.61265608778258	40.0	37.0	40.0	33.0	40.0
41	37.59017594541766	40.0	37.0	40.0	33.0	40.0
42	37.52765246984252	40.0	37.0	40.0	33.0	40.0
43	37.450089251640385	40.0	37.0	40.0	33.0	40.0
44	37.23498543933009	40.0	36.0	40.0	33.0	40.0
45	37.469396111774515	40.0	37.0	40.0	33.0	40.0
46	37.48584122919684	40.0	37.0	40.0	33.0	40.0
47	37.45427386096441	40.0	37.0	40.0	33.0	40.0
48	37.29732069995171	40.0	36.0	40.0	33.0	40.0
49	37.24286491610346	40.0	36.0	40.0	33.0	40.0
50	37.14459449662759	40.0	36.0	40.0	33.0	40.0
51	36.501337447799756	39.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	68.0
9	172.0
10	227.0
11	266.0
12	277.0
13	256.0
14	343.0
15	382.0
16	601.0
17	1019.0
18	1841.0
19	3455.0
20	6034.0
21	9788.0
22	14936.0
23	21467.0
24	29603.0
25	39554.0
26	51025.0
27	65431.0
28	81733.0
29	102183.0
30	127072.0
31	157173.0
32	198108.0
33	255589.0
34	350844.0
35	517745.0
36	871095.0
37	1771743.0
38	3741726.0
39	9855214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.836597686433342	47.106744961898784	18.217752117076266	22.83890523459161
2	23.885982226271032	41.97479068609548	19.690085476398583	14.4491416112349
3	32.8895016817608	24.754402738965283	22.760314548769696	19.595781030504227
4	19.44040520928655	25.303727777709383	22.718540677546475	32.53732633545759
5	18.516275258898148	36.79058487523236	25.169274455975344	19.523865409894142
6	30.52467927542714	24.05408991367554	24.25909648615266	21.16213432474466
7	21.402748540171444	24.705603635174857	34.14985247832313	19.74179534633056
8	29.382947670497988	24.909931758400937	25.573953020125046	20.133167550976022
9	30.221587543890603	24.170690171650396	25.68540472370291	19.92231756075609
10	19.981714698411526	25.260361739292197	34.96495453979666	19.79296902249962
11	19.667472325152275	35.77889120054057	24.74910098635031	19.80453548795684
12	19.987114935595194	24.734147746002826	35.14729871874312	20.13143859965886
13	19.86198481969801	25.44170465845688	24.285676376971573	30.410634144873537
14	20.058663974755124	35.93920766428799	24.17736523686223	19.82476312409466
15	19.923745587002227	35.63742906033411	24.149390147986743	20.289435204676924
16	20.269968088264058	25.026214677209985	24.057897983665242	30.645919250860715
17	30.90986336633934	24.744926334910456	24.29171129248686	20.053499006263344
18	20.231887388367056	24.647158515016844	24.136351885363965	30.984602211252138
19	20.591377949578636	35.34017527517546	24.037900144854387	20.030546630391516
20	30.97038760516989	25.06120405592282	24.066176158800896	19.90223218010639
21	20.5131976161263	35.241072442239485	24.078114677073202	20.16761526456101
22	30.809359863981868	24.501477952451104	24.393538865156845	20.295623318410186
23	20.22055619160173	24.882454563733877	34.93923912463632	19.957750120028066
24	20.306281537289404	35.038768724036665	24.171653131877676	20.483296606796255
25	20.128516890787452	24.96140635964102	24.15975838452479	30.75031836504673
26	20.38556708645714	29.497643893478	30.202377581629346	19.91441143843552
27	20.428648613898087	24.517820919490234	34.78928542606203	20.264245040549653
28	25.043367406258422	24.812278837170812	29.90799079169283	20.23636296487794
29	25.15377407913509	24.80107348179882	29.979775099544646	20.065377339521447
30	25.14769539270038	24.519670240835804	29.985290235391798	20.347344131072017
31	23.203708491148014	25.616038759149145	30.30237771841347	20.877875031289367
32	22.28073299875937	26.203433555060396	30.64786158541006	20.867971860770176
33	22.20588472654802	25.631604792368538	30.75929140352821	21.40321907755523
34	21.67687486578058	25.930100577365785	30.794390209539596	21.59863434731404
35	21.50838965419606	25.854026719410623	31.25708165601802	21.380501970375295
36	21.700708131405772	25.67965979052879	31.163258690237306	21.45637338782813
37	22.218315667663823	25.71585834089066	30.93882330090182	21.1270026905437
38	21.933919590085342	25.868558664658675	31.183037674451054	21.014484070804933
39	22.061106939195355	25.63674240403568	31.1004145926774	21.20173606409157
40	21.577985416076785	26.258601327626703	30.804506763290973	21.358906493005545
41	21.80221289354502	26.25807060522871	30.831546248763814	21.108170252462454
42	21.81291488334366	25.99326748545643	30.938194093935127	21.255623537264785
43	21.643811407522307	26.196731133023928	31.027109245375673	21.132348214078096
44	22.005014505956265	25.880967720314768	31.063351566656955	21.050666207072013
45	21.93033037469275	25.553916881759704	31.011794894942955	21.503957848604596
46	21.677558786396546	25.842832306768486	30.75172997719809	21.72787892963688
47	21.639718826556365	25.907985320327896	30.96580807272538	21.486487780390355
48	21.747860354352948	25.62777483691913	30.874868516261582	21.74949629246634
49	21.50124952296537	26.32274213867448	30.580974149168554	21.59503418919159
50	21.91490112559655	26.17567732078202	30.628192028494865	21.28122952512656
51	21.789595926021672	26.16936883702035	30.550143007800802	21.490892229157176
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	50675.0
1	30142.5
2	9610.0
3	7305.5
4	5001.0
5	4769.5
6	4538.0
7	4770.0
8	5002.0
9	5698.5
10	6395.0
11	8665.0
12	10935.0
13	14569.5
14	18204.0
15	23830.0
16	29456.0
17	40791.0
18	52126.0
19	68375.5
20	84625.0
21	118318.0
22	152011.0
23	635281.0
24	1118551.0
25	740079.0
26	375939.0
27	390271.0
28	424011.0
29	457751.0
30	489997.5
31	522244.0
32	561754.0
33	601264.0
34	653936.0
35	706608.0
36	772577.0
37	838546.0
38	884370.0
39	930194.0
40	979309.0
41	1028424.0
42	1060219.5
43	1092015.0
44	1116976.5
45	1141938.0
46	1162111.0
47	1182284.0
48	1185372.5
49	1188461.0
50	1174391.5
51	1160322.0
52	1127532.0
53	1094742.0
54	1057222.0
55	1019702.0
56	945606.5
57	871511.0
58	776276.0
59	681041.0
60	594239.5
61	507438.0
62	436073.5
63	364709.0
64	306568.0
65	248427.0
66	198626.5
67	148826.0
68	120266.5
69	91707.0
70	69894.0
71	48081.0
72	36578.0
73	25075.0
74	18744.5
75	9480.0
76	6546.0
77	4936.0
78	3326.0
79	2598.5
80	1871.0
81	1706.5
82	1542.0
83	1061.0
84	580.0
85	382.0
86	184.0
87	132.0
88	80.0
89	69.5
90	59.0
91	41.0
92	23.0
93	19.0
94	15.0
95	11.0
96	7.0
97	7.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.8276975E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.537361039133714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	52.798928295184666	18.235356490101253
2	21.664988287354454	14.96503044777928
3	10.329587954888543	10.702701257504174
4	5.337601352988567	7.373866600445389
5	2.9725312986141357	5.13316933301807
6	1.7467107346649628	3.619606756443259
7	1.0984723977351514	2.655683645447127
8	0.7503502088255033	2.073209285439663
9	0.5039061388552223	1.5663229422730789
>10	2.5140319876203265	16.05506813922644
>50	0.17081295805995622	4.029877407215259
>100	0.1092111405718501	6.630307151359877
>500	0.0017605408566816506	0.3860272131616312
>1k	9.390470635245358E-4	0.692095752553015
>5k	3.353134331022495E-5	0.09242090240113408
>10k+	1.341253732408998E-4	5.789256675631317
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	858711	4.6983212484560495	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47159	0.2580240986268242	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT	28353	0.15512960979593177	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG	19469	0.10652200377797748	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6414094783190325E-5	5.471364927730109E-6	0.0	0.0	0.0
2	1.6414094783190325E-5	5.471364927730109E-6	0.0	0.0	0.0
3	1.6414094783190325E-5	5.471364927730109E-6	0.0	0.0	0.0
4	2.735682463865054E-5	5.471364927730109E-6	0.0	0.0	0.0
5	2.735682463865054E-5	5.471364927730109E-6	0.0	0.0	0.0
6	5.471364927730108E-5	5.471364927730109E-6	0.0	0.0	0.0
7	5.471364927730108E-5	5.471364927730109E-6	0.0	0.0	0.0
8	5.471364927730108E-5	5.471364927730109E-6	0.0	0.0	0.0
9	5.471364927730108E-5	5.471364927730109E-6	0.0	0.0	0.0
10	5.471364927730108E-5	5.471364927730109E-6	0.0	0.0	0.0
11	5.471364927730108E-5	5.471364927730109E-6	0.0	0.0	0.0
12	5.471364927730108E-5	5.471364927730109E-6	0.0	0.0	0.0
13	7.112774406049141E-5	5.471364927730109E-6	0.0	0.0	0.0
14	8.754183884368174E-5	5.471364927730109E-6	0.0	0.0	0.0
15	8.754183884368174E-5	5.471364927730109E-6	0.0	0.0	0.0
16	8.754183884368174E-5	5.471364927730109E-6	0.0	0.0	0.0
17	1.0395593362687206E-4	5.471364927730109E-6	0.0	0.0	0.0
18	1.367841231932527E-4	5.471364927730109E-6	0.0	0.0	0.0
19	1.5319821797644303E-4	5.471364927730109E-6	0.0	0.0	0.0
20	1.5866958290417315E-4	5.471364927730109E-6	0.0	0.0	0.0
21	1.6961231275963335E-4	5.471364927730109E-6	0.0	0.0	0.0
22	1.969691373982839E-4	5.471364927730109E-6	0.0	0.0	0.0
23	2.2432596203693445E-4	5.471364927730109E-6	0.0	0.0	0.0
24	2.51682786675585E-4	5.471364927730109E-6	0.0	0.0	0.0
25	3.00925071025156E-4	5.471364927730109E-6	0.0	0.0	0.0
26	3.6111008523018716E-4	5.471364927730109E-6	0.0	0.0	0.0
27	4.1582373450748826E-4	5.471364927730109E-6	0.0	0.0	0.0
28	5.635505875562012E-4	5.471364927730109E-6	0.0	0.0	5.471364927730109E-6
29	6.401496965444227E-4	5.471364927730109E-6	0.0	0.0	5.471364927730109E-6
30	7.222201704603743E-4	5.471364927730109E-6	0.0	0.0	5.471364927730109E-6
31	8.59004293653627E-4	5.471364927730109E-6	0.0	0.0	5.471364927730109E-6
32	0.001055973431051911	5.471364927730109E-6	0.0	0.0	5.471364927730109E-6
33	0.0011708720945342432	1.0942729855460217E-5	0.0	0.0	5.471364927730109E-6
34	0.001411612151354368	1.0942729855460217E-5	0.0	0.0	5.471364927730109E-6
35	0.0015046253551257798	1.0942729855460217E-5	0.0	5.471364927730109E-6	5.471364927730109E-6
36	0.0015921671939694616	1.0942729855460217E-5	0.0	5.471364927730109E-6	5.471364927730109E-6
37	0.0016851803977408733	1.0942729855460217E-5	0.0	5.471364927730109E-6	5.471364927730109E-6
38	0.001767250871656825	1.0942729855460217E-5	0.0	5.471364927730109E-6	5.471364927730109E-6
39	0.0018985636299223476	1.0942729855460217E-5	0.0	5.471364927730109E-6	5.471364927730109E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	200060	0.0	43.281513	1
TCAACGC	201285	0.0	43.23832	12
CAACGCA	200820	0.0	43.209587	13
AGTGGTA	201940	0.0	43.05239	5
AACGCAG	201765	0.0	43.03732	14
GGTATCA	202380	0.0	43.03328	8
ACGCAGA	201790	0.0	43.002995	15
GTGGTAT	201685	0.0	42.990803	6
ATCAACG	202390	0.0	42.98224	11
GTATCAA	202740	0.0	42.97906	9
TGGTATC	201845	0.0	42.971214	7
CGCAGAG	200220	0.0	42.82664	16
TATCAAC	204185	0.0	42.662777	10
AGAGTAC	199370	0.0	42.58038	19
CAGTGGT	204180	0.0	42.393845	4
GCAGTGG	204765	0.0	42.300198	3
GAGTACT	110260	0.0	42.2227	20
CAGAGTA	202040	0.0	42.203648	18
GCAGAGT	202645	0.0	42.18646	17
AGCAGTG	205255	0.0	42.127964	2
>>END_MODULE