FastQCFastQC Report
Thu 26 May 2016
SRR805294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20173591
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6473203.2087494982920988No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT536880.2661301104002753No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC472620.23427658466953155No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG385320.191002186968101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2582650.043.4745371
TCAACGC2585800.043.34512
CAACGCA2594150.043.30436313
GTGGTAT2591550.043.2983176
AGTGGTA2592700.043.267835
GTATCAA2590450.043.2541669
GGTATCA2592150.043.242298
TGGTATC2595250.043.2105837
ACGCAGA2601900.043.19526315
ATCAACG2594150.043.18293411
AACGCAG2602450.043.17835614
TATCAAC2604650.042.98034310
CGCAGAG2595050.042.9659416
AGAGTAC2582000.042.80664419
AGCAGTG2642500.042.529052
CAGTGGT2644800.042.5065274
CAGAGTA2607800.042.4789118
GCAGTGG2652550.042.423053
GCAGAGT2629250.042.28981817
GAGTACA1729950.042.11393720