##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805294_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20173591
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.348122701605284	37.0	37.0	37.0	35.0	37.0
2	36.27408798958995	37.0	37.0	37.0	35.0	37.0
3	38.06520851939548	39.0	39.0	39.0	35.0	39.0
4	38.0176117380391	39.0	38.0	39.0	35.0	39.0
5	37.981864904468424	39.0	38.0	39.0	35.0	39.0
6	37.99281565686545	39.0	38.0	39.0	35.0	39.0
7	37.85574957874381	39.0	38.0	39.0	35.0	39.0
8	38.767162722789415	40.0	39.0	40.0	36.0	40.0
9	38.748100821514626	40.0	39.0	40.0	36.0	40.0
10	38.57709284380753	40.0	38.0	40.0	36.0	40.0
11	38.54932128841117	40.0	38.0	40.0	36.0	40.0
12	38.50476987463462	40.0	38.0	40.0	36.0	40.0
13	38.6430538321115	40.0	39.0	40.0	36.0	40.0
14	38.55762496622441	40.0	38.0	40.0	36.0	40.0
15	38.50715710455317	40.0	38.0	40.0	36.0	40.0
16	38.46780253451158	40.0	38.0	40.0	36.0	40.0
17	38.28242215280363	40.0	38.0	40.0	35.0	40.0
18	38.27349151670617	40.0	38.0	40.0	35.0	40.0
19	38.185757161429514	40.0	38.0	40.0	34.0	40.0
20	37.984251638689415	40.0	38.0	40.0	34.0	40.0
21	37.67404038279551	39.0	37.0	40.0	34.0	40.0
22	37.25613099819462	39.0	37.0	40.0	33.0	40.0
23	37.24735392920378	39.0	37.0	40.0	33.0	40.0
24	37.2735371704522	39.0	37.0	40.0	33.0	40.0
25	37.13368561898573	38.0	36.0	40.0	33.0	40.0
26	37.11650042870404	38.0	36.0	40.0	33.0	40.0
27	37.01984401289785	38.0	36.0	40.0	33.0	40.0
28	36.810083043717896	38.0	36.0	40.0	32.0	40.0
29	36.54968790633259	38.0	36.0	40.0	31.0	40.0
30	36.315587492578786	38.0	35.0	40.0	31.0	40.0
31	35.88689366211499	38.0	35.0	40.0	30.0	40.0
32	35.83611063592991	38.0	35.0	39.0	30.0	40.0
33	35.61559907703096	38.0	34.0	39.0	30.0	40.0
34	35.431175044641286	38.0	34.0	39.0	30.0	40.0
35	35.29817933753093	37.0	34.0	39.0	29.0	40.0
36	34.994744069114915	37.0	33.0	39.0	29.0	40.0
37	34.71443879277616	37.0	33.0	38.0	29.0	40.0
38	34.431426660726885	36.0	33.0	38.0	28.0	40.0
39	34.162366234152366	36.0	33.0	38.0	27.0	39.0
40	33.8842895149406	35.0	32.0	38.0	27.0	39.0
41	33.78748448900347	35.0	32.0	38.0	27.0	39.0
42	33.64311961117879	35.0	32.0	38.0	26.0	39.0
43	33.57117575150602	35.0	32.0	38.0	26.0	39.0
44	33.301722137620416	35.0	31.0	38.0	26.0	39.0
45	33.00321152540467	35.0	31.0	38.0	25.0	39.0
46	32.75744610862786	34.0	31.0	37.0	24.0	39.0
47	32.448591775257064	34.0	30.0	37.0	24.0	39.0
48	32.14139564939133	34.0	30.0	37.0	24.0	38.0
49	31.58274756338621	34.0	29.0	37.0	23.0	38.0
50	31.397641252863707	33.0	29.0	36.0	22.0	38.0
51	32.419322965356045	34.0	31.0	37.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	16.0
8	136.0
9	295.0
10	374.0
11	412.0
12	414.0
13	445.0
14	563.0
15	828.0
16	1354.0
17	2494.0
18	4733.0
19	8862.0
20	16266.0
21	26686.0
22	41669.0
23	60065.0
24	82499.0
25	108455.0
26	139218.0
27	174241.0
28	213673.0
29	260475.0
30	315691.0
31	388304.0
32	488874.0
33	652951.0
34	953157.0
35	1614802.0
36	3459431.0
37	7040809.0
38	4006154.0
39	109245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.26223521632812	37.71200179482175	26.109134461980517	21.91662852686961
2	25.042185102295374	39.30014244861017	17.70226728597799	17.955405163116474
3	35.35242188661404	23.45050516787021	19.944822912291617	21.25225003322413
4	21.03925870213191	22.742435890566036	20.940892476703826	35.27741293059823
5	21.28086665383471	35.416119024124164	22.42755888131171	20.875455440729418
6	34.10449334478923	22.681534487340404	21.46418552849614	21.749786639374218
7	21.882152761003233	22.33113083337518	34.82343326976343	20.963283135858163
8	33.34458401580561	23.230990456780848	22.546283405864628	20.87814212154891
9	33.31677042525548	22.817400233800715	22.890242991443614	20.975586349500194
10	20.8793912794207	23.23555087440803	35.012764955926784	20.872292890244477
11	20.776558818903386	35.49572309659693	22.857155178768124	20.870562905731557
12	20.8193771748421	22.990606878071436	35.186903511625665	21.003112435460796
13	20.764156465747718	23.145532196027965	22.84494119068836	33.24537014753595
14	20.99822485743862	35.556594757968476	22.617445748751425	20.827734635841484
15	20.802007932053346	35.44340717525204	22.614169187825805	21.140415704868808
16	21.01826591011982	22.90320548285132	22.741370140794466	33.3371584662344
17	33.53612155614734	22.74811162772161	22.646300304194728	21.069466511936323
18	21.071652538211964	22.828097387321872	22.637511586311035	33.46273848815513
19	21.091386258400895	35.233196707517266	22.661156360312848	21.014260673768987
20	33.59154054426899	22.95008360187336	22.620405063233413	20.83797079062424
21	21.19296460407074	35.1395445659625	22.618570982231176	21.048919847735586
22	33.36074375652803	22.681009047918142	22.899824825436383	21.058422370117448
23	21.158597891669363	22.827160518918024	35.113153627433014	20.9010879619796
24	21.074859701478037	35.016219967976944	22.67322659609784	21.235693734447178
25	20.89657711410923	22.996976591822445	22.72600847315681	33.38043782091151
26	21.168169811710765	30.91074365491003	26.915609620518232	21.005476912860978
27	21.256205699818143	22.82588657616782	34.9081628550911	21.00974486892294
28	29.299111893365936	22.75312313013583	26.90890778939654	21.03885718710169
29	29.4449709028006	22.819611044954762	26.748946184147382	20.986471868097254
30	29.274089080124604	22.7644944323497	26.765393429459337	21.19602305806636
31	26.260872444573703	23.791079139058585	27.588434800725363	22.359613615642353
32	24.69567763121598	25.211966476370023	27.911431336146354	22.180924556267648
33	24.27210901618854	24.710429590844782	28.196784598240342	22.82067679472633
34	23.562522904325757	24.372805020187034	28.681695787329087	23.382976288158115
35	23.304512320092144	24.710578300115234	28.830191907826425	23.154717471966197
36	23.515089604027363	24.751775725006024	28.75644698060945	22.97668769035716
37	23.98318673160371	24.479072664851785	28.750429212131838	22.787311391412665
38	23.985511553198435	24.61166680736216	28.74291939397403	22.65990224546537
39	24.027427739563077	24.588304580974203	28.664440554981013	22.719827124481704
40	23.476682956445387	24.974388545896463	28.42304079625685	23.1258877014013
41	23.775296128487984	25.268386773579376	28.26172593664658	22.69459116128606
42	23.63946012388176	25.23056009215216	28.47581275936446	22.65416702460162
43	23.54354760141613	24.771677982368136	29.079840074084977	22.604934342130758
44	23.765426789905675	24.555390262447574	28.65449686176348	23.02468608588327
45	23.871312747442932	24.279668404103166	28.557295525620603	23.291723322833302
46	23.58578599120008	24.400673137469674	28.393789682759007	23.619751188571236
47	23.496109344142052	24.516299552221515	28.50979282766266	23.477798275973772
48	23.386163623521465	24.52059725013757	28.52373184327966	23.569507283061306
49	23.178545654068234	25.222901564723898	28.25308592803334	23.34546685317453
50	23.755839998937226	24.968608712251577	28.220012986284893	23.05553830252631
51	23.560252609463532	24.70466958510262	28.425350746924533	23.309727058509317
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6498.0
1	4147.5
2	1797.0
3	1516.0
4	1235.0
5	1239.0
6	1243.0
7	1432.0
8	1621.0
9	2085.0
10	2549.0
11	3801.0
12	5053.0
13	7207.0
14	9361.0
15	12630.0
16	15899.0
17	22997.0
18	30095.0
19	40807.5
20	51520.0
21	70582.0
22	89644.0
23	453758.0
24	817872.0
25	546022.0
26	272402.0
27	270632.0
28	293382.0
29	316132.0
30	343680.0
31	371228.0
32	405938.5
33	440649.0
34	490573.0
35	540497.0
36	605254.0
37	670011.0
38	731653.0
39	793295.0
40	868102.0
41	942909.0
42	1014794.0
43	1086679.0
44	1170282.5
45	1253886.0
46	1344879.0
47	1435872.0
48	1467617.0
49	1499362.0
50	1528821.0
51	1558280.0
52	1558819.5
53	1559359.0
54	1535870.5
55	1512382.0
56	1412297.5
57	1312213.0
58	1166372.5
59	1020532.0
60	901501.0
61	782470.0
62	681608.5
63	580747.0
64	493615.0
65	406483.0
66	320262.5
67	234042.0
68	190068.0
69	146094.0
70	108039.0
71	69984.0
72	50748.0
73	31512.0
74	23216.0
75	11009.0
76	7098.0
77	5154.5
78	3211.0
79	2684.5
80	2158.0
81	1838.0
82	1518.0
83	1090.0
84	662.0
85	407.5
86	153.0
87	94.0
88	35.0
89	26.0
90	17.0
91	11.0
92	5.0
93	4.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.0173591E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.34156799943696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.91419740390673	33.32616669118077
2	14.881884551114124	13.79299729770451
3	5.155782015582717	7.167810685962019
4	2.3465227719144144	4.349661783875965
5	1.3106149609393996	3.0367976166726303
6	0.7997797512478853	2.2237828636215986
7	0.5505218325567756	1.7858431457023123
8	0.39805687244531435	1.4757263697654193
9	0.3170457252114678	1.3223156430436236
>10	2.173423942334968	18.147897031348535
>50	0.10300296308819122	3.223065872997826
>100	0.044361340113111686	3.7449320752323274
>500	0.003830217840203899	1.2052706164090166
>1k	7.784679528166191E-4	0.6570761518098067
>5k	1.205011817003193E-4	0.37466520375229034
>10k+	7.66825701729272E-5	4.165990950921277
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	647320	3.2087494982920988	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT	53688	0.2661301104002753	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC	47262	0.23427658466953155	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG	38532	0.191002186968101	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7845112454198164E-4	0.0	0.0	4.956975681721712E-6	4.956975681721712E-6
2	2.428918084043639E-4	9.913951363443424E-6	0.0	4.956975681721712E-6	9.913951363443424E-6
3	2.478487840860856E-4	9.913951363443424E-6	0.0	4.956975681721712E-6	3.4698829772051985E-5
4	2.478487840860856E-4	9.913951363443424E-6	0.0	4.956975681721712E-6	5.452673249893884E-5
5	2.478487840860856E-4	9.913951363443424E-6	0.0	4.956975681721712E-6	7.435463522582569E-5
6	2.775906381764159E-4	9.913951363443424E-6	0.0	4.956975681721712E-6	1.0409648931615595E-4
7	2.775906381764159E-4	9.913951363443424E-6	0.0	4.956975681721712E-6	1.0905346499787767E-4
8	2.825476138581376E-4	9.913951363443424E-6	0.0	4.956975681721712E-6	1.189674163613211E-4
9	2.825476138581376E-4	9.913951363443424E-6	0.0	4.956975681721712E-6	1.239243920430428E-4
10	2.825476138581376E-4	9.913951363443424E-6	0.0	4.956975681721712E-6	1.239243920430428E-4
11	2.92461565221581E-4	9.913951363443424E-6	0.0	1.4870927045165137E-5	1.3879531908820794E-4
12	2.92461565221581E-4	9.913951363443424E-6	0.0	1.4870927045165137E-5	1.4870927045165138E-4
13	2.9741854090330276E-4	9.913951363443424E-6	0.0	1.982790272688685E-5	1.586232218150948E-4
14	3.27160394993633E-4	9.913951363443424E-6	0.0	2.4784878408608562E-5	1.8836507590542506E-4
15	3.321173706753547E-4	9.913951363443424E-6	0.0	2.4784878408608562E-5	2.0323600295059022E-4
16	3.569022490839633E-4	9.913951363443424E-6	0.0	2.4784878408608562E-5	2.3297785704092048E-4
17	3.668162004474067E-4	9.913951363443424E-6	0.0	2.4784878408608562E-5	2.478487840860856E-4
18	4.114289815829021E-4	9.913951363443424E-6	0.0	2.4784878408608562E-5	2.676766868129725E-4
19	4.560417627183975E-4	9.913951363443424E-6	0.0	2.9741854090330275E-5	2.825476138581376E-4
20	5.006545438538929E-4	9.913951363443424E-6	0.0	2.9741854090330275E-5	3.0733249226674614E-4
21	5.155254708990581E-4	9.913951363443424E-6	0.0	2.9741854090330275E-5	3.222034193119113E-4
22	5.502243006711101E-4	9.913951363443424E-6	0.0	2.9741854090330275E-5	3.321173706753547E-4
23	6.047510331700489E-4	9.913951363443424E-6	0.0	3.4698829772051985E-5	3.4203132203879815E-4
24	6.394498629421009E-4	9.913951363443424E-6	0.0	3.4698829772051985E-5	3.668162004474067E-4
25	7.435463522582568E-4	9.913951363443424E-6	0.0	3.4698829772051985E-5	3.8168712749257186E-4
26	8.625137686195779E-4	9.913951363443424E-6	0.0	3.96558054537737E-5	4.114289815829021E-4
27	9.319114281636819E-4	9.913951363443424E-6	0.0	3.96558054537737E-5	4.163859572646238E-4
28	0.0010013090877077858	9.913951363443424E-6	0.0	3.96558054537737E-5	4.362138599915107E-4
29	0.0011103625527056635	9.913951363443424E-6	0.0	3.96558054537737E-5	4.5108478703667583E-4
30	0.0012144590420218196	1.4870927045165137E-5	0.0	3.96558054537737E-5	4.7091268976356265E-4
31	0.001412738069290688	1.4870927045165137E-5	0.0	3.96558054537737E-5	4.808266411270061E-4
32	0.0016605868533767737	1.4870927045165137E-5	0.0	3.96558054537737E-5	4.956975681721712E-4
33	0.0018192100751918685	1.4870927045165137E-5	0.0	3.96558054537737E-5	5.204824465807798E-4
34	0.002017489102460737	1.982790272688685E-5	0.0	4.461278113549541E-5	5.204824465807798E-4
35	0.0022008972026844402	1.982790272688685E-5	0.0	4.461278113549541E-5	5.502243006711101E-4
36	0.002359520424499535	1.982790272688685E-5	0.0	4.461278113549541E-5	5.700522033979969E-4
37	0.00251814364631463	1.982790272688685E-5	0.0	5.452673249893884E-5	5.799661547614403E-4
38	0.0027610354547189936	2.9741854090330275E-5	0.0	6.444068386238226E-5	5.84923130443162E-4
39	0.0030435830685771313	2.9741854090330275E-5	0.0	6.444068386238226E-5	5.84923130443162E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	258265	0.0	43.474537	1
TCAACGC	258580	0.0	43.345	12
CAACGCA	259415	0.0	43.304363	13
GTGGTAT	259155	0.0	43.298317	6
AGTGGTA	259270	0.0	43.26783	5
GTATCAA	259045	0.0	43.254166	9
GGTATCA	259215	0.0	43.24229	8
TGGTATC	259525	0.0	43.210583	7
ACGCAGA	260190	0.0	43.195263	15
ATCAACG	259415	0.0	43.182934	11
AACGCAG	260245	0.0	43.178356	14
TATCAAC	260465	0.0	42.980343	10
CGCAGAG	259505	0.0	42.96594	16
AGAGTAC	258200	0.0	42.806644	19
AGCAGTG	264250	0.0	42.52905	2
CAGTGGT	264480	0.0	42.506527	4
CAGAGTA	260780	0.0	42.47891	18
GCAGTGG	265255	0.0	42.42305	3
GCAGAGT	262925	0.0	42.289818	17
GAGTACA	172995	0.0	42.113937	20
>>END_MODULE