FastQCFastQC Report
Thu 26 May 2016
SRR805293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33045913
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1271060.38463455374950606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC728800.037.6619112
ATCAACG730100.037.5723211
CAACGCA733500.037.45262513
GTGGTAT743950.036.8766
AGTGGTA744950.036.8517345
GGTATCA749900.036.5834128
AACGCAG751950.036.5336814
ACGCAGA751900.036.53298615
GTATCAA752750.036.4199269
CGCAGAG761500.036.0755116
TATCAAC762700.035.97869510
AGAGTAC773200.035.23480219
AAGCAGT760600.034.9348531
TGGTATC789200.034.8003657
CAGTGGT817250.033.6260644
CAGAGTA814750.033.61098518
GTACATG572000.032.11940822
GCAGTGG857850.032.1085853
ACATGGG574000.031.70452924
TACATGG584450.031.41509223
Produced by FastQC (version 0.11.5)