FastQCFastQC Report
Thu 26 May 2016
SRR805292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23657477
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2597591.0979995880372195No Hit
CAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTC1683990.7118214676907432Illumina Paired End PCR Primer 2 (100% over 52bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1948800.043.3594812
ATCAACG1949350.043.3327811
AAGCAGT1936000.043.3232731
GTGGTAT1947150.043.315366
CAACGCA1951550.043.2959713
AGTGGTA1950050.043.247335
GGTATCA1955900.043.1095668
GTATCAA1956150.043.098059
AACGCAG1966650.042.98744614
TATCAAC1963900.042.94951610
ACGCAGA1969650.042.91242215
TGGTATC1971300.042.7918667
CGCAGAG1979700.042.70525716
AGAGTAC1968400.042.50988419
CAGTGGT2004850.042.0593574
CAGAGTA1999300.041.990418
GCAGTGG2011450.041.7531133
GTACATG1568250.041.60695322
ACATGGG1559100.041.42608324
AGCAGTG2036200.041.362172
Produced by FastQC (version 0.11.5)