##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR805292_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23657477 Sequences flagged as poor quality 0 Sequence length 53 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.739207270496344 37.0 37.0 37.0 37.0 37.0 2 36.75279616672564 37.0 37.0 37.0 37.0 37.0 3 38.37283214942997 39.0 39.0 39.0 38.0 39.0 4 38.51850943361374 39.0 39.0 39.0 38.0 39.0 5 38.576222434877565 39.0 39.0 39.0 38.0 39.0 6 38.61196128395264 39.0 39.0 39.0 38.0 39.0 7 38.55524242927511 39.0 39.0 39.0 37.0 39.0 8 39.53126370998903 40.0 40.0 40.0 39.0 40.0 9 39.48359019856598 40.0 40.0 40.0 39.0 40.0 10 39.32804639311284 40.0 40.0 40.0 38.0 40.0 11 39.42628015658644 40.0 40.0 40.0 38.0 40.0 12 39.50462037858052 40.0 40.0 40.0 39.0 40.0 13 39.57011497887116 40.0 40.0 40.0 39.0 40.0 14 39.555002885557066 40.0 40.0 40.0 39.0 40.0 15 39.511365053847456 40.0 40.0 40.0 39.0 40.0 16 39.508808906376615 40.0 40.0 40.0 39.0 40.0 17 39.497176431789406 40.0 40.0 40.0 39.0 40.0 18 39.48393529031012 40.0 40.0 40.0 39.0 40.0 19 39.47969963153721 40.0 40.0 40.0 39.0 40.0 20 39.456104976874755 40.0 40.0 40.0 39.0 40.0 21 39.41351594677657 40.0 40.0 40.0 39.0 40.0 22 39.38843613797025 40.0 40.0 40.0 39.0 40.0 23 39.172168866527905 40.0 40.0 40.0 38.0 40.0 24 39.223456245989375 40.0 40.0 40.0 38.0 40.0 25 39.3072011017912 40.0 40.0 40.0 38.0 40.0 26 39.319325978843814 40.0 40.0 40.0 38.0 40.0 27 39.31148642773699 40.0 40.0 40.0 38.0 40.0 28 39.255857672396765 40.0 40.0 40.0 38.0 40.0 29 39.25384351002434 40.0 40.0 40.0 38.0 40.0 30 39.219596366932954 40.0 40.0 40.0 38.0 40.0 31 39.164355100081046 40.0 40.0 40.0 38.0 40.0 32 39.14654982016891 40.0 40.0 40.0 38.0 40.0 33 39.062836920437455 40.0 40.0 40.0 38.0 40.0 34 39.02838933331733 40.0 40.0 40.0 37.0 40.0 35 39.03919509252825 40.0 40.0 40.0 37.0 40.0 36 39.003416594254745 40.0 40.0 40.0 37.0 40.0 37 38.91877747572153 40.0 40.0 40.0 37.0 40.0 38 38.85522391081686 40.0 40.0 40.0 36.0 40.0 39 38.823901213134434 40.0 39.0 40.0 36.0 40.0 40 38.91530681822073 40.0 40.0 40.0 36.0 40.0 41 38.91495940163019 40.0 40.0 40.0 36.0 40.0 42 38.89457798056826 40.0 40.0 40.0 36.0 40.0 43 38.88800648522241 40.0 40.0 40.0 36.0 40.0 44 38.81301873399264 40.0 39.0 40.0 36.0 40.0 45 38.75256097681084 40.0 39.0 40.0 35.0 40.0 46 38.68365109263342 40.0 39.0 40.0 35.0 40.0 47 38.65109462010678 40.0 39.0 40.0 35.0 40.0 48 38.56174202346261 40.0 39.0 40.0 35.0 40.0 49 38.49851579692965 40.0 39.0 40.0 35.0 40.0 50 38.39177292658892 40.0 38.0 40.0 35.0 40.0 51 38.24920797767234 40.0 38.0 40.0 35.0 40.0 52 38.05897585782288 40.0 38.0 40.0 35.0 40.0 53 37.823545258017155 40.0 37.0 40.0 35.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 14.0 13 50.0 14 163.0 15 430.0 16 858.0 17 1583.0 18 2160.0 19 2749.0 20 3775.0 21 5385.0 22 7916.0 23 11490.0 24 16531.0 25 22346.0 26 29596.0 27 38094.0 28 48482.0 29 60966.0 30 75752.0 31 93506.0 32 117257.0 33 149034.0 34 203392.0 35 313863.0 36 525444.0 37 1137184.0 38 2721442.0 39 1.8068014E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.71308237983281 32.243899042996006 37.22957862328261 19.813439953888572 2 28.205150532324303 40.89836798742317 14.384991265129413 16.51149021512311 3 33.53197384488633 26.148845035334915 20.029659122145613 20.289521997633138 4 22.19921422728214 25.523038657080804 20.747199711955762 31.530547403681297 5 21.195497727843083 32.86368618259674 24.40481290545057 21.536003184109614 6 29.555002843287138 24.61650073674382 21.898979337483876 23.92951708248517 7 23.19836557380992 23.662869882532274 31.432130315502366 21.706634228155437 8 29.469680980774072 25.27202710584903 23.686697444533074 21.571594468843823 9 28.953125474876295 25.364771568836353 24.108800782095233 21.573302174192115 10 21.48309390726661 25.764678963864153 31.21082607414138 21.541401054727856 11 21.937220947102688 32.370235422822134 24.37311890866469 21.31942472141049 12 21.002235360938954 25.57922808083043 31.787802224218588 21.630734334012033 13 21.812528867723298 24.842116511409902 24.472867499776076 28.872487121090725 14 21.52224854746768 32.29853716015448 24.032372513772284 22.146841778605555 15 21.947725025792057 32.234849050048744 24.091554648874858 21.72587127528434 16 22.229931788584217 24.56691810373524 24.244990283621537 28.958159824059003 17 29.091331252271747 24.323324925984288 24.88312680172953 21.702217020014434 18 21.453851566673826 24.55354389650257 24.874875710541748 29.11772882628186 19 21.484150655625704 32.07284952659998 24.11263043814858 22.33036937962573 20 29.27447842388265 25.462787092638827 24.01187582259934 21.250858660879178 21 22.477079867815153 31.918750253883793 24.028986692029754 21.5751831862713 22 28.982224097692246 24.47174734651544 24.2897118741783 22.25631668161402 23 21.715870208813897 25.2624698736894 31.605310236590316 21.41634968090638 24 22.288547506566317 31.796450652789392 24.130383810581325 21.78461803006297 25 22.15059534877705 24.656488094651852 24.22795550007298 28.964961056498122 26 21.72664058808976 31.03768842298779 25.725363697912503 21.51030729100994 27 21.75185460393769 25.186248728044834 31.504872645548804 21.557024022468667 28 27.5561337331111 24.37129707449362 26.5263409111631 21.54622828123219 29 28.505546047873153 24.36689254733292 25.60652177744905 21.521039627344877 30 27.61269090528969 24.343711715327885 25.635273786803214 22.408323592579208 31 24.988932674435233 25.349161282075855 26.2727931638695 23.38911287961941 32 24.659438536070436 26.499812300356457 26.590595438389308 22.250153725183797 33 23.78500040388922 26.72678916690905 26.710946395509545 22.777264033692184 34 24.219666365944263 25.818010940050794 26.995148299203674 22.96717439480127 35 23.29784363734138 26.523229843993928 27.0443758647636 23.13455065390109 36 23.060379600073162 25.98401765327723 27.05916611479745 23.896436631852165 37 23.359642281381063 26.12510624019628 27.043297981437327 23.47195349698533 38 24.36896800111018 25.96006750846677 26.980201650412678 22.690762840010372 39 23.343431761552594 26.480283590680443 27.196399683702538 22.979884964064425 40 23.071517727777987 25.94322716661629 27.914663089390302 23.070592016215425 41 23.275402529187705 26.13318402465318 27.026871884943603 23.56454156121551 42 23.45367597736648 25.910294660753554 27.8148891363183 22.82114022556167 43 23.337281486102682 25.818137749853882 27.305870359717566 23.538710404325872 44 23.986425095118978 25.927105413649986 27.169063717149548 22.917405774081487 45 23.20412696586369 25.805465223531655 27.933299903451243 23.057107907153412 46 23.201083530589507 26.53640538253509 27.164405570382677 23.098105516492733 47 23.469307399094163 25.898404128217056 27.738760984529332 22.893527488159453 48 24.00718808687841 25.923719591907457 27.003149997778713 23.065942323435422 49 23.086612321339253 26.046687057964803 27.013227150131016 23.85347347056493 50 23.413084159397048 25.856852782737565 26.90515560894342 23.824907448921962 51 23.991569346131033 25.82154047957016 26.961446480535518 23.225443693763285 52 23.3029371644322 25.77777841652345 27.751289793074722 23.167994625969623 53 23.456744774601283 25.6595874530492 26.788499044086567 24.09516872826295 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 153.0 1 142.0 2 131.0 3 134.0 4 137.0 5 173.0 6 209.0 7 254.0 8 414.0 9 529.0 10 881.0 11 1233.0 12 2164.5 13 3096.0 14 5124.0 15 7152.0 16 12114.5 17 17077.0 18 27250.0 19 37423.0 20 53613.0 21 69803.0 22 236920.5 23 404038.0 24 305104.0 25 226010.0 26 245850.0 27 278649.0 28 311448.0 29 357096.5 30 402745.0 31 455952.0 32 509159.0 33 574395.5 34 639632.0 35 720188.0 36 800744.0 37 887329.0 38 973914.0 39 1075125.5 40 1176337.0 41 1270108.5 42 1461698.0 43 1559516.0 44 1628138.0 45 1696760.0 46 1740761.0 47 1784762.0 48 1817297.5 49 1849833.0 50 1827501.0 51 1805169.0 52 1756534.0 53 1707899.0 54 1613804.0 55 1519709.0 56 1430077.0 57 1340445.0 58 1266101.5 59 966903.0 60 742048.0 61 631292.5 62 520537.0 63 427825.5 64 335114.0 65 268379.5 66 201645.0 67 158321.0 68 114997.0 69 87528.0 70 60059.0 71 44795.5 72 29532.0 73 21647.0 74 13762.0 75 10341.0 76 4900.5 77 2881.0 78 2077.0 79 1273.0 80 1059.0 81 845.0 82 641.0 83 437.0 84 318.0 85 199.0 86 144.0 87 89.0 88 66.5 89 44.0 90 38.5 91 33.0 92 30.5 93 20.5 94 13.0 95 9.5 96 6.0 97 4.5 98 3.0 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 53 2.3657477E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.596788485657626 #Duplication Level Percentage of deduplicated Percentage of total 1 70.25847061860624 36.25111447834017 2 16.407114608260766 16.931088442047482 3 5.58932485994982 8.651736377293764 4 2.6516629846394886 5.472691766147652 5 1.4425679942166993 3.721593783688921 6 0.8945869178926033 2.7694687190724614 7 0.5647728904706504 2.039832716043334 8 0.4009374052130955 1.6549665994214795 9 0.2685180490028768 1.2469202081084592 >10 1.418417285625004 13.104602149669583 >50 0.07349798191767082 2.5577022560613885 >100 0.028851891452364487 2.541021107096309 >500 8.881312188059606E-4 0.30752120048247716 >1k 2.6209218298602874E-4 0.2450953707452368 >5k 5.412400749531302E-5 0.18111569221729767 >10k+ 7.216534332689319E-5 2.3235291335639485 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 259759 1.0979995880372195 No Hit CAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTC 168399 0.7118214676907432 Illumina Paired End PCR Primer 2 (100% over 52bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.026236948259529113 0.0 0.0 0.0 0.0 2 0.7842340922491439 0.0 0.0 0.0 0.0 3 0.7848047363630534 4.226993436366862E-6 0.0 4.226993436366862E-6 4.226993436366862E-6 4 0.7884653126789472 4.226993436366862E-6 0.0 8.453986872733723E-6 4.226993436366862E-6 5 0.7885667605214199 4.226993436366862E-6 0.0 1.2680980309100586E-5 8.453986872733723E-6 6 0.7892895763990386 4.226993436366862E-6 0.0 2.536196061820117E-5 8.453986872733723E-6 7 0.7898898094670027 4.226993436366862E-6 0.0 2.9588954054568033E-5 8.453986872733723E-6 8 0.7911156375635492 4.226993436366862E-6 0.0 3.381594749093489E-5 8.453986872733723E-6 9 0.7943112446014425 4.226993436366862E-6 0.0 3.804294092730176E-5 8.453986872733723E-6 10 0.7992652809088645 4.226993436366862E-6 0.0 3.804294092730176E-5 1.2680980309100586E-5 11 0.8052380226344509 4.226993436366862E-6 0.0 3.804294092730176E-5 2.113496718183431E-5 12 0.8122717397125653 4.226993436366862E-6 0.0 4.226993436366862E-5 2.113496718183431E-5 13 0.8217571129837725 4.226993436366862E-6 0.0 4.649692780003548E-5 2.113496718183431E-5 14 0.8364459151751473 4.226993436366862E-6 0.0 6.340490154550293E-5 2.536196061820117E-5 15 0.8581346184971458 1.6907973745467447E-5 0.0 6.340490154550293E-5 2.536196061820117E-5 16 0.8752285799538133 1.6907973745467447E-5 0.0 7.185888841823666E-5 2.9588954054568033E-5 17 0.8843927017238566 1.6907973745467447E-5 0.0 7.185888841823666E-5 3.381594749093489E-5 18 0.8913418789332438 1.6907973745467447E-5 0.0 8.031287529097037E-5 3.381594749093489E-5 19 0.8974837003962849 1.6907973745467447E-5 0.0 8.031287529097037E-5 3.381594749093489E-5 20 0.9023362888612341 1.6907973745467447E-5 0.0 8.031287529097037E-5 3.381594749093489E-5 21 0.9060940860261641 1.6907973745467447E-5 0.0 8.453986872733724E-5 3.381594749093489E-5 22 0.9087021409764026 1.6907973745467447E-5 0.0 8.87668621637041E-5 3.381594749093489E-5 23 0.9103168524690947 1.6907973745467447E-5 0.0 8.87668621637041E-5 3.381594749093489E-5 24 0.9118005271652594 1.6907973745467447E-5 0.0 9.722084903643783E-5 3.381594749093489E-5 25 0.9125825209509872 1.6907973745467447E-5 0.0 1.0144784247280469E-4 3.381594749093489E-5 26 0.9135674104216608 2.113496718183431E-5 0.0 1.0144784247280469E-4 3.381594749093489E-5 27 0.9145522998923342 2.9588954054568033E-5 0.0 1.0144784247280469E-4 3.381594749093489E-5 28 0.9156639991660988 2.9588954054568033E-5 0.0 1.0144784247280469E-4 3.804294092730176E-5 29 0.9160909255031718 2.9588954054568033E-5 0.0 1.0567483590917154E-4 4.226993436366862E-5 30 0.9176464590877548 2.9588954054568033E-5 0.0 1.1412882278190527E-4 4.226993436366862E-5 31 0.9285351941798359 2.9588954054568033E-5 0.0 1.22582809654639E-4 4.226993436366862E-5 32 0.929055114372509 2.9588954054568033E-5 0.0 1.22582809654639E-4 4.226993436366862E-5 33 0.9294778137161457 2.9588954054568033E-5 0.0 1.2680980309100586E-4 5.072392123640234E-5 34 0.9297694762632549 2.9588954054568033E-5 0.0 1.3526378996373957E-4 5.072392123640234E-5 35 0.9300400038431824 2.9588954054568033E-5 0.0 1.3949078340010643E-4 5.072392123640234E-5 36 0.9303316663902917 2.9588954054568033E-5 0.0 1.563987571455739E-4 5.072392123640234E-5 37 0.9311094331825832 3.381594749093489E-5 0.0 1.563987571455739E-4 5.9177908109136065E-5 38 0.9313672797822016 3.381594749093489E-5 0.0 1.563987571455739E-4 6.340490154550293E-5 39 0.9317392552046019 3.381594749093489E-5 0.0 1.563987571455739E-4 6.340490154550293E-5 40 0.9319675128501658 3.381594749093489E-5 0.0 1.648527440183076E-4 6.340490154550293E-5 41 0.9322253594497841 3.381594749093489E-5 0.0 1.648527440183076E-4 6.763189498186979E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAACGC 194880 0.0 43.35948 12 ATCAACG 194935 0.0 43.33278 11 AAGCAGT 193600 0.0 43.323273 1 GTGGTAT 194715 0.0 43.31536 6 CAACGCA 195155 0.0 43.29597 13 AGTGGTA 195005 0.0 43.24733 5 GGTATCA 195590 0.0 43.109566 8 GTATCAA 195615 0.0 43.09805 9 AACGCAG 196665 0.0 42.987446 14 TATCAAC 196390 0.0 42.949516 10 ACGCAGA 196965 0.0 42.912422 15 TGGTATC 197130 0.0 42.791866 7 CGCAGAG 197970 0.0 42.705257 16 AGAGTAC 196840 0.0 42.509884 19 CAGTGGT 200485 0.0 42.059357 4 CAGAGTA 199930 0.0 41.9904 18 GCAGTGG 201145 0.0 41.753113 3 GTACATG 156825 0.0 41.606953 22 ACATGGG 155910 0.0 41.426083 24 AGCAGTG 203620 0.0 41.36217 2 >>END_MODULE