Basic Statistics
Measure | Value |
---|---|
Filename | SRR805291_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 35658795 |
Sequences flagged as poor quality | 0 |
Sequence length | 53 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
ATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTC | 190531 | 0.5343169896795447 | Illumina Paired End PCR Primer 2 (100% over 53bp) |
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAA | 130771 | 0.36672860089635667 | Illumina Paired End PCR Primer 2 (96% over 33bp) |
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTG | 49041 | 0.13752848350596256 | Illumina Paired End PCR Primer 2 (100% over 53bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 28760 | 0.0 | 33.011124 | 42 |
CTCGTAT | 29100 | 0.0 | 32.536594 | 38 |
ACCGATC | 29395 | 0.0 | 32.242046 | 32 |
CGTATGC | 29685 | 0.0 | 31.95873 | 40 |
TCGTATG | 29780 | 0.0 | 31.912022 | 39 |
ATGCCGT | 29815 | 0.0 | 31.677507 | 43 |
TCTCGTA | 30070 | 0.0 | 31.48703 | 37 |
GACCGAT | 30255 | 0.0 | 31.473143 | 31 |
CCGATCT | 30225 | 0.0 | 31.185608 | 33 |
CGATCTC | 30685 | 0.0 | 30.77937 | 34 |
GCCGTCT | 30775 | 0.0 | 30.712263 | 45 |
AGACCGA | 31345 | 0.0 | 30.446163 | 30 |
ATCTCGT | 31160 | 0.0 | 30.415756 | 36 |
GATCTCG | 31195 | 0.0 | 30.389162 | 35 |
TGCCGTC | 31145 | 0.0 | 30.332315 | 44 |
GAGACCG | 31775 | 0.0 | 30.063728 | 29 |
CGAGACC | 31910 | 0.0 | 29.980724 | 28 |
GTATGCC | 32010 | 0.0 | 29.776943 | 41 |
CCGTCTT | 33000 | 0.0 | 28.74121 | 46 |
CCGAGAC | 33515 | 0.0 | 28.678204 | 27 |