FastQCFastQC Report
Thu 26 May 2016
SRR805291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35658795
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTC1905310.5343169896795447Illumina Paired End PCR Primer 2 (100% over 53bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAA1307710.36672860089635667Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTG490410.13752848350596256Illumina Paired End PCR Primer 2 (100% over 53bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG287600.033.01112442
CTCGTAT291000.032.53659438
ACCGATC293950.032.24204632
CGTATGC296850.031.9587340
TCGTATG297800.031.91202239
ATGCCGT298150.031.67750743
TCTCGTA300700.031.4870337
GACCGAT302550.031.47314331
CCGATCT302250.031.18560833
CGATCTC306850.030.7793734
GCCGTCT307750.030.71226345
AGACCGA313450.030.44616330
ATCTCGT311600.030.41575636
GATCTCG311950.030.38916235
TGCCGTC311450.030.33231544
GAGACCG317750.030.06372829
CGAGACC319100.029.98072428
GTATGCC320100.029.77694341
CCGTCTT330000.028.7412146
CCGAGAC335150.028.67820427