##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805291_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35658795
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.68230979201625	37.0	37.0	37.0	37.0	37.0
2	36.69320676708229	37.0	37.0	37.0	37.0	37.0
3	38.32910758762319	39.0	39.0	39.0	38.0	39.0
4	38.472757562334905	39.0	39.0	39.0	38.0	39.0
5	38.55033376758805	39.0	39.0	39.0	38.0	39.0
6	38.569156641440074	39.0	39.0	39.0	38.0	39.0
7	38.576322671587754	39.0	39.0	39.0	38.0	39.0
8	39.51636621484265	40.0	40.0	40.0	39.0	40.0
9	39.42860803344588	40.0	40.0	40.0	38.0	40.0
10	39.47712475982433	40.0	40.0	40.0	39.0	40.0
11	39.45626970849688	40.0	40.0	40.0	39.0	40.0
12	39.45082594630581	40.0	40.0	40.0	39.0	40.0
13	39.51627170800359	40.0	40.0	40.0	39.0	40.0
14	39.50178941829078	40.0	40.0	40.0	39.0	40.0
15	39.45828304069165	40.0	40.0	40.0	39.0	40.0
16	39.46242633829887	40.0	40.0	40.0	39.0	40.0
17	39.4589621157978	40.0	40.0	40.0	39.0	40.0
18	39.43467632599475	40.0	40.0	40.0	39.0	40.0
19	39.4321551807906	40.0	40.0	40.0	39.0	40.0
20	39.393379024725874	40.0	40.0	40.0	39.0	40.0
21	39.367975586387594	40.0	40.0	40.0	38.0	40.0
22	39.319273772431174	40.0	40.0	40.0	38.0	40.0
23	39.3301136227402	40.0	40.0	40.0	38.0	40.0
24	39.255455771850954	40.0	40.0	40.0	38.0	40.0
25	39.26741130764514	40.0	40.0	40.0	38.0	40.0
26	39.261725164857644	40.0	40.0	40.0	38.0	40.0
27	39.236288270537464	40.0	40.0	40.0	38.0	40.0
28	39.1650592511609	40.0	40.0	40.0	38.0	40.0
29	39.162470773339365	40.0	40.0	40.0	38.0	40.0
30	39.1098018034541	40.0	40.0	40.0	38.0	40.0
31	39.09355167497948	40.0	40.0	40.0	38.0	40.0
32	39.098073841250105	40.0	40.0	40.0	38.0	40.0
33	39.043026019247144	40.0	40.0	40.0	38.0	40.0
34	39.005997622746364	40.0	40.0	40.0	37.0	40.0
35	39.005451109607044	40.0	40.0	40.0	37.0	40.0
36	38.990592811675214	40.0	40.0	40.0	37.0	40.0
37	38.928539957673834	40.0	40.0	40.0	37.0	40.0
38	38.87599628647014	40.0	40.0	40.0	37.0	40.0
39	38.852457717654225	40.0	40.0	40.0	37.0	40.0
40	38.93850622265839	40.0	40.0	40.0	37.0	40.0
41	38.945753270686794	40.0	40.0	40.0	37.0	40.0
42	38.91888539699673	40.0	40.0	40.0	37.0	40.0
43	38.91502550773239	40.0	40.0	40.0	37.0	40.0
44	38.83620781352819	40.0	40.0	40.0	36.0	40.0
45	38.803241808928206	40.0	39.0	40.0	36.0	40.0
46	38.72695299995415	40.0	39.0	40.0	36.0	40.0
47	38.692028320082045	40.0	39.0	40.0	36.0	40.0
48	38.612613802569605	40.0	39.0	40.0	35.0	40.0
49	38.54724241242588	40.0	39.0	40.0	35.0	40.0
50	38.4791826252121	40.0	39.0	40.0	35.0	40.0
51	38.3781230969807	40.0	38.0	40.0	35.0	40.0
52	38.25976259152896	40.0	38.0	40.0	35.0	40.0
53	38.058044558151785	40.0	38.0	40.0	35.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	1.0
11	9.0
12	18.0
13	61.0
14	209.0
15	602.0
16	1362.0
17	2283.0
18	3610.0
19	5431.0
20	7797.0
21	10620.0
22	15208.0
23	22097.0
24	30824.0
25	42359.0
26	55489.0
27	70737.0
28	88489.0
29	109661.0
30	133562.0
31	163765.0
32	200794.0
33	247638.0
34	318990.0
35	450604.0
36	693716.0
37	1308043.0
38	3867540.0
39	2.7807275E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.504769300252576	21.488314453699292	39.39787084785114	25.609045398196994
2	28.50124913082453	24.8444233743737	27.731110936306173	18.923216558495596
3	20.919669327020166	24.526353175983655	30.095974920072315	24.45800257692387
4	20.843449701539267	28.134430790496427	28.36261853492245	22.659500973041855
5	25.19291243576795	28.279598343129653	26.59346452957819	19.934024691524208
6	24.18119008227844	27.06797860107163	26.148645796920505	22.602185519729424
7	23.646076655142164	27.83025057352611	27.590121875963558	20.93355089536817
8	23.186420068316945	25.485075981956207	27.343921744972032	23.984582204754815
9	21.9172184590085	25.268506689583877	28.780178915187683	24.03409593621994
10	24.25707318489029	25.759061684501678	26.751133906796348	23.232731223811683
11	23.209892538432662	26.348161792904108	26.9699270544616	23.47201861420163
12	23.095460741171987	26.228698978751247	27.594067606603083	23.081772673473683
13	23.329585309879374	26.13779573875113	27.092000725206784	23.44061822616272
14	23.002103688585102	26.416826479974997	26.77797160560249	23.803098225837413
15	22.844437115724187	26.901657781761834	26.85599162843276	23.397913474081218
16	23.74726347314877	26.012382078530692	26.0570554893961	24.18329895892444
17	23.755292347932677	26.41748550392687	25.847828004283375	23.97939414385708
18	23.745496728086295	26.632032854727704	25.85779749427876	23.764672922907238
19	23.971379290859378	26.098949782234648	25.98154256194019	23.948128364965783
20	24.196740243185445	26.61362505379108	25.925777918182597	23.26385678484088
21	23.768040955954906	26.898407531718334	26.07918186803564	23.25436964429112
22	24.21329716834234	26.360988922929113	26.150709803850635	23.275004104877915
23	23.457912697274264	26.429089934194355	26.883622960338393	23.229374408192985
24	23.775820242944274	26.620989295908625	26.04223446137201	23.56095599977509
25	23.187530593784786	25.889725101479172	26.58287247227507	24.339871832460968
26	24.053193609038107	25.929117907657844	26.165446701157457	23.852241782146592
27	23.924215610763067	25.713106682376676	26.42626594645164	23.936411760408618
28	23.866378547003624	26.251417076768856	26.016627875395116	23.865576500832404
29	24.975796293733428	25.772250576610904	25.994341648392773	23.257611481262895
30	23.612811369537305	26.866482728875162	26.121477183959808	23.399228717627725
31	24.187704604151655	25.750687873777	26.370147953681556	23.69145956838979
32	23.727198297082108	26.491430795684483	25.878863825880828	23.90250708135258
33	24.12296040850511	25.846260368585085	25.93777215410672	24.093007068803082
34	24.00631877773772	26.0807074383753	25.842805400462915	24.07016838342406
35	24.148202428040545	26.364699087560307	26.025371861275737	23.46172662312341
36	23.5530617341388	26.775074704571484	25.946911554358472	23.72495200693125
37	23.969264805498895	25.98219878153482	26.51016109770395	23.538375315262336
38	23.67808558870259	26.266647540950277	25.874601202872956	24.180665667474184
39	24.096537754570786	25.716586889714023	26.539780718894175	23.647094636821013
40	23.624135364080587	25.73751861216847	26.091983758845466	24.546362264905476
41	24.643833309566407	25.890364494930353	25.99879777205035	23.467004423452895
42	23.90554420024569	25.734234709838066	26.863420370766878	23.496800719149373
43	23.614311700661787	26.312389411924887	26.484773251591932	23.5885256358214
44	23.578491645609446	25.767777626809877	26.914790586726223	23.73894014085445
45	24.064955083311144	25.756955612212916	26.01082005154689	24.167269252929046
46	23.741430410085364	26.07189334356363	25.946050616685167	24.24062562966584
47	23.983572636147688	25.75071591734942	26.037618489351644	24.228092957151244
48	24.094271833919233	25.821910134652615	25.890089667920634	24.193728363507518
49	23.657739976911728	26.163935152604008	26.54098098379376	23.637343886690505
50	24.150684284199734	25.82498090583263	25.999978406449237	24.0243564035184
51	23.945248290078226	25.806805866547087	26.487420564828394	23.76052527854629
52	23.6415279876956	26.206199620598507	26.535201203517957	23.617071188187936
53	23.74925737114785	25.933658162032675	26.03608450594026	24.280999960879214
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	600.0
1	471.5
2	343.0
3	320.5
4	298.0
5	367.5
6	437.0
7	511.0
8	806.5
9	1028.0
10	1527.5
11	2027.0
12	3274.5
13	4522.0
14	7707.0
15	10892.0
16	18400.0
17	25908.0
18	41317.5
19	56727.0
20	78320.5
21	99914.0
22	140777.5
23	181641.0
24	213714.0
25	292937.0
26	340087.0
27	397401.5
28	454716.0
29	534346.5
30	613977.0
31	703019.5
32	792062.0
33	898300.5
34	1004539.0
35	1135978.0
36	1267417.0
37	1405825.5
38	1544234.0
39	1711875.5
40	1879517.0
41	2032112.0
42	2321799.0
43	2458891.0
44	2534015.0
45	2609139.0
46	2644236.0
47	2679333.0
48	2688355.0
49	2697377.0
50	2692695.5
51	2688014.0
52	2628254.0
53	2568494.0
54	2436992.0
55	2305490.0
56	2257640.5
57	2209791.0
58	1979757.0
59	1431694.0
60	1113665.0
61	935682.5
62	757700.0
63	622249.0
64	486798.0
65	390096.5
66	293395.0
67	229076.0
68	164757.0
69	125213.0
70	85669.0
71	63516.5
72	41364.0
73	30390.5
74	19417.0
75	14606.0
76	6927.0
77	4059.0
78	2990.5
79	1922.0
80	1465.0
81	1008.0
82	725.0
83	442.0
84	344.5
85	247.0
86	201.0
87	155.0
88	109.0
89	63.0
90	61.5
91	60.0
92	46.0
93	23.0
94	14.0
95	11.0
96	8.0
97	6.5
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	3.5658795E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.858263231198485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.68070977996828	31.714005145441032
2	16.802829327725206	15.747027993348988
3	6.134645822975646	8.623765464094953
4	3.0135943620144388	5.64847191549313
5	1.7334293027591363	4.0612743280680235
6	1.0601655271797925	2.980650920473982
7	0.7132296114910601	2.3394490615673464
8	0.4816707944354254	1.8056205501148523
9	0.3754693680189561	1.5834458233695585
>10	1.8396233066024035	15.473332611290298
>50	0.1160395412932545	3.703249444678959
>100	0.04556308087884946	3.900826750614029
>500	0.002484661637741227	0.7675197027247014
>1k	4.924570423743854E-4	0.3434950075017724
>5k	1.3263994550719076E-5	0.036191560726183215
>10k+	3.979198299563329E-5	1.2716737204921222
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTC	190531	0.5343169896795447	Illumina Paired End PCR Primer 2 (100% over 53bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAA	130771	0.36672860089635667	Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTG	49041	0.13752848350596256	Illumina Paired End PCR Primer 2 (100% over 53bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0014133960499786938	0.0	0.0	5.608714484042436E-6	0.0
2	0.0038083171346648143	0.0	0.0	8.413071726063654E-6	0.0
3	0.003970969854702045	0.0	0.0	1.1217428968084872E-5	2.804357242021218E-6
4	0.004128013860255233	2.804357242021218E-6	0.0	1.9630500694148526E-5	2.804357242021218E-6
5	0.0169439264562922	2.804357242021218E-6	0.0	2.5239215178190963E-5	5.608714484042436E-6
6	0.02451569100974949	2.804357242021218E-6	0.0	2.5239215178190963E-5	5.608714484042436E-6
7	0.02580849969832127	2.804357242021218E-6	0.0	2.5239215178190963E-5	1.1217428968084872E-5
8	0.02631328400188509	2.804357242021218E-6	0.0	2.804357242021218E-5	1.1217428968084872E-5
9	0.026941460024097842	8.413071726063654E-6	0.0	3.3652286904254615E-5	1.1217428968084872E-5
10	0.027757527981526017	1.1217428968084872E-5	0.0	3.3652286904254615E-5	1.1217428968084872E-5
11	0.02896059723835312	1.1217428968084872E-5	0.0	3.3652286904254615E-5	1.1217428968084872E-5
12	0.03069088565668021	1.1217428968084872E-5	0.0	3.926100138829705E-5	1.1217428968084872E-5
13	0.03371117840633706	1.1217428968084872E-5	0.0	4.206535863031827E-5	1.1217428968084872E-5
14	0.03588455526890351	1.402178621010609E-5	0.0	4.486971587233949E-5	1.402178621010609E-5
15	0.041277334245310306	1.402178621010609E-5	0.0	4.767407311436071E-5	1.402178621010609E-5
16	0.045295978173126715	1.6826143452127308E-5	0.0	4.767407311436071E-5	1.9630500694148526E-5
17	0.05287896015555209	2.2434857936169745E-5	0.0	5.0478430356381926E-5	1.9630500694148526E-5
18	0.05723412695241104	2.2434857936169745E-5	0.0	5.0478430356381926E-5	1.9630500694148526E-5
19	0.0614855325313152	2.2434857936169745E-5	0.0	5.0478430356381926E-5	2.2434857936169745E-5
20	0.06708302958638955	2.2434857936169745E-5	0.0	5.0478430356381926E-5	2.5239215178190963E-5
21	0.07215330747996392	2.804357242021218E-5	0.0	5.0478430356381926E-5	3.08479296622334E-5
22	0.07933526637678026	3.08479296622334E-5	0.0	5.608714484042436E-5	3.08479296622334E-5
23	0.08986001910608589	3.3652286904254615E-5	0.0	6.16958593244668E-5	3.08479296622334E-5
24	0.10714888150314669	3.3652286904254615E-5	0.0	7.291328829255167E-5	3.08479296622334E-5
25	0.13521769313853707	3.3652286904254615E-5	0.0	8.132636001861533E-5	3.08479296622334E-5
26	0.18234491659070365	3.3652286904254615E-5	0.0	8.693507450265775E-5	3.08479296622334E-5
27	0.2943313143363369	3.3652286904254615E-5	5.608714484042436E-6	8.973943174467898E-5	3.3652286904254615E-5
28	0.7338890728079847	3.3652286904254615E-5	5.608714484042436E-6	9.254378898670019E-5	3.3652286904254615E-5
29	0.7419684260222478	3.6456644146275834E-5	5.608714484042436E-6	9.254378898670019E-5	3.3652286904254615E-5
30	0.7547142296872342	3.926100138829705E-5	5.608714484042436E-6	9.815250347074263E-5	3.3652286904254615E-5
31	0.774768188324928	4.206535863031827E-5	5.608714484042436E-6	1.0095686071276385E-4	3.3652286904254615E-5
32	0.7753991687043827	4.486971587233949E-5	5.608714484042436E-6	1.093699324388275E-4	3.6456644146275834E-5
33	0.7757469090023934	4.767407311436071E-5	5.608714484042436E-6	1.1217428968084873E-4	3.926100138829705E-5
34	0.776223649733537	5.3282787598403145E-5	5.608714484042436E-6	1.1217428968084873E-4	3.926100138829705E-5
35	0.7771631094096141	5.3282787598403145E-5	5.608714484042436E-6	1.1217428968084873E-4	4.206535863031827E-5
36	0.7776286327117896	5.889150208244558E-5	5.608714484042436E-6	1.1497864692286994E-4	4.486971587233949E-5
37	0.7781446344443215	6.16958593244668E-5	5.608714484042436E-6	1.1778300416489116E-4	4.486971587233949E-5
38	0.7785344401009625	6.450021656648802E-5	5.608714484042436E-6	1.1778300416489116E-4	4.767407311436071E-5
39	0.7789074196141513	6.450021656648802E-5	5.608714484042436E-6	1.1778300416489116E-4	4.767407311436071E-5
40	0.7793841603452949	6.450021656648802E-5	5.608714484042436E-6	1.1778300416489116E-4	4.767407311436071E-5
41	0.7799562492226673	6.730457380850923E-5	5.608714484042436E-6	1.1778300416489116E-4	4.767407311436071E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	28760	0.0	33.011124	42
CTCGTAT	29100	0.0	32.536594	38
ACCGATC	29395	0.0	32.242046	32
CGTATGC	29685	0.0	31.95873	40
TCGTATG	29780	0.0	31.912022	39
ATGCCGT	29815	0.0	31.677507	43
TCTCGTA	30070	0.0	31.48703	37
GACCGAT	30255	0.0	31.473143	31
CCGATCT	30225	0.0	31.185608	33
CGATCTC	30685	0.0	30.77937	34
GCCGTCT	30775	0.0	30.712263	45
AGACCGA	31345	0.0	30.446163	30
ATCTCGT	31160	0.0	30.415756	36
GATCTCG	31195	0.0	30.389162	35
TGCCGTC	31145	0.0	30.332315	44
GAGACCG	31775	0.0	30.063728	29
CGAGACC	31910	0.0	29.980724	28
GTATGCC	32010	0.0	29.776943	41
CCGTCTT	33000	0.0	28.74121	46
CCGAGAC	33515	0.0	28.678204	27
>>END_MODULE