FastQCFastQC Report
Thu 26 May 2016
SRR805290_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805290_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25158463
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1961700.7797376175166185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1620750.043.03149812
ATCAACG1619950.043.0092311
AAGCAGT1614200.042.957161
CAACGCA1625350.042.92706313
AGTGGTA1627000.042.81425
GTGGTAT1630050.042.7038156
GGTATCA1631600.042.6690068
GTATCAA1632600.042.6212849
AACGCAG1641700.042.49954614
TATCAAC1637800.042.4988710
ACGCAGA1644950.042.40700515
CGCAGAG1654900.042.17475516
AGAGTAC1644750.041.99352619
TGGTATC1663250.041.862717
CAGTGGT1687550.041.2766154
CAGAGTA1684600.041.1396518
GTACATG1343500.040.87793422
ACATGGG1334400.040.7305224
TACATGG1348350.040.6664123
GCAGTGG1708150.040.6426283
Produced by FastQC (version 0.11.5)