##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805290_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25158463
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.753629981290985	37.0	37.0	37.0	37.0	37.0
2	36.75346089306012	37.0	37.0	37.0	37.0	37.0
3	38.37804841257592	39.0	39.0	39.0	38.0	39.0
4	38.52412637449275	39.0	39.0	39.0	38.0	39.0
5	38.58547447830974	39.0	39.0	39.0	38.0	39.0
6	38.614852107618816	39.0	39.0	39.0	38.0	39.0
7	38.57463740928848	39.0	39.0	39.0	38.0	39.0
8	39.54621587972206	40.0	40.0	40.0	39.0	40.0
9	39.49088396218799	40.0	40.0	40.0	39.0	40.0
10	39.38316772371985	40.0	40.0	40.0	38.0	40.0
11	39.45685016608527	40.0	40.0	40.0	39.0	40.0
12	39.5171520613163	40.0	40.0	40.0	39.0	40.0
13	39.57909165595689	40.0	40.0	40.0	39.0	40.0
14	39.566129059632935	40.0	40.0	40.0	39.0	40.0
15	39.52009619983542	40.0	40.0	40.0	39.0	40.0
16	39.51776056430792	40.0	40.0	40.0	39.0	40.0
17	39.51764767982846	40.0	40.0	40.0	39.0	40.0
18	39.49662064014006	40.0	40.0	40.0	39.0	40.0
19	39.49281961302644	40.0	40.0	40.0	39.0	40.0
20	39.466104268770316	40.0	40.0	40.0	39.0	40.0
21	39.428262052415526	40.0	40.0	40.0	39.0	40.0
22	39.40113742242521	40.0	40.0	40.0	39.0	40.0
23	39.237824425124856	40.0	40.0	40.0	38.0	40.0
24	39.25902746920589	40.0	40.0	40.0	38.0	40.0
25	39.3265516657357	40.0	40.0	40.0	38.0	40.0
26	39.335545657141296	40.0	40.0	40.0	38.0	40.0
27	39.32424333712278	40.0	40.0	40.0	38.0	40.0
28	39.269758251925005	40.0	40.0	40.0	38.0	40.0
29	39.267515507604735	40.0	40.0	40.0	38.0	40.0
30	39.23032356944858	40.0	40.0	40.0	38.0	40.0
31	39.18881141506935	40.0	40.0	40.0	38.0	40.0
32	39.17273133895342	40.0	40.0	40.0	38.0	40.0
33	39.11391733270828	40.0	40.0	40.0	38.0	40.0
34	39.08098551171429	40.0	40.0	40.0	38.0	40.0
35	39.08235097668725	40.0	40.0	40.0	38.0	40.0
36	39.06742053359937	40.0	40.0	40.0	38.0	40.0
37	38.99023517454147	40.0	40.0	40.0	37.0	40.0
38	38.92818730619593	40.0	40.0	40.0	37.0	40.0
39	38.90369399752282	40.0	40.0	40.0	37.0	40.0
40	38.97886957561756	40.0	40.0	40.0	37.0	40.0
41	38.98728658424006	40.0	40.0	40.0	37.0	40.0
42	38.965189924360644	40.0	40.0	40.0	37.0	40.0
43	38.95709149640819	40.0	40.0	40.0	37.0	40.0
44	38.886178142122596	40.0	40.0	40.0	36.0	40.0
45	38.84905830694029	40.0	39.0	40.0	36.0	40.0
46	38.77560819196308	40.0	39.0	40.0	36.0	40.0
47	38.73547771976372	40.0	39.0	40.0	35.0	40.0
48	38.65077175024563	40.0	39.0	40.0	35.0	40.0
49	38.59063604163736	40.0	39.0	40.0	35.0	40.0
50	38.49707329100351	40.0	39.0	40.0	35.0	40.0
51	38.37121345608434	40.0	38.0	40.0	35.0	40.0
52	38.21966810929587	40.0	38.0	40.0	35.0	40.0
53	37.988204327108534	40.0	38.0	40.0	35.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	13.0
13	38.0
14	109.0
15	309.0
16	687.0
17	1245.0
18	1841.0
19	2575.0
20	3628.0
21	5611.0
22	8410.0
23	12031.0
24	17142.0
25	23658.0
26	31241.0
27	40628.0
28	51664.0
29	64140.0
30	79063.0
31	98022.0
32	121471.0
33	154331.0
34	206841.0
35	313790.0
36	509060.0
37	1059683.0
38	2646573.0
39	1.9704656E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.783644096223208	31.3141625543659	33.24530993805146	21.65688341135943
2	26.102731315502066	34.87200311084187	19.62939071436916	19.395874859286913
3	30.198863897210256	25.45155083599503	22.10888638149318	22.240698885301537
4	22.430551500701775	25.448172251222186	22.289799659065025	29.831476589011025
5	23.000534651103287	31.54552008999914	23.862232760403526	21.591712498494044
6	29.220799378721985	25.1280096085361	22.74902485100143	22.902166161740485
7	23.0044299606061	24.718799395654656	30.611472568892623	21.66529807484662
8	28.0368439041765	25.717004254194702	24.32477294022294	21.92137890140586
9	27.75909641220928	25.19326001751379	24.95682665511005	22.090816915166876
10	21.82304618529359	25.49044828374452	30.54333247623275	22.143173054729136
11	21.758133634793193	31.260295988669895	24.960133693381824	22.021436683155088
12	21.57589674695151	25.336841920748498	30.827952407108494	22.259308925191494
13	21.599968169756632	25.406540137209493	25.03931579604048	27.954175896993387
14	21.969195812955665	31.26310617623978	24.813443492156097	21.954254518648455
15	21.741606393045554	31.119440802087155	24.897105200742985	22.241847604124306
16	21.87634832859225	25.171728495496726	24.997000015461992	27.95492316044903
17	28.014465748563417	24.947946939365888	24.879532585118575	22.15805472695212
18	21.928243390703162	25.14113839148282	24.87355845227906	28.057059765534966
19	22.024600628424718	31.04524310566985	24.890805133843035	22.0393511320624
20	28.091851239084043	25.155634507561132	24.833154553201442	21.919359700153386
21	22.099772152217724	30.98336333185378	24.815081112069524	22.10178340385897
22	27.967841278698145	25.009683620179818	24.990747646229423	22.031727454892614
23	22.122949243759447	25.094458274338937	30.757681818638922	22.024910663262695
24	22.026945763737636	30.86488630088412	24.833985287575	22.274182647803244
25	21.96123030250298	25.194384887502864	24.93304141830922	27.91134339168494
26	22.22896923393134	29.72533735467067	26.075984848517976	21.969708562880015
27	22.254892916153103	24.970428439924966	30.638950400109895	22.135728243812032
28	26.932591231825253	25.01853551228467	25.989159194661454	22.05971406122862
29	27.03375400953548	24.973508914276678	25.936946148101335	22.055790928086505
30	26.91951412135153	24.96297567939663	25.954920219092877	22.162589980158963
31	24.918537352619673	25.757062345183808	26.41584265302693	22.908557649169587
32	23.91510959950137	26.73996420210567	26.685469617122475	22.659456581270483
33	23.82145125479247	26.325125664473227	26.737316981566007	23.1161060991683
34	23.680468874430048	26.18943375038451	26.971059400568308	23.15903797461713
35	23.49081499931057	26.077876855990766	26.999220898351382	23.432087246347283
36	23.254254443127152	26.325789457010945	26.969656294186173	23.45029980567573
37	23.480245991179984	26.512792136785144	26.96924688920782	23.03771498282705
38	23.76411070898886	26.296161255955898	26.90458872626678	23.03513930878846
39	23.487909416405923	26.104782315199458	27.108301488846916	23.2990067795477
40	23.299940858867252	26.270229624122905	27.09154768317921	23.338281833830628
41	23.45360286914189	26.41584662783255	26.99077046161365	23.13978004141191
42	23.665368587898236	26.214546572260794	27.030144886036954	23.089939953804013
43	23.548091948224343	26.157830070938754	27.207063483965616	23.08701449687129
44	23.47650172429055	26.27004678306461	27.099203158793923	23.15424833385092
45	23.392772444008205	26.185164809153882	27.117928468046713	23.304134278791196
46	23.388189493133982	26.201485361009535	27.130258315064797	23.28006683079169
47	23.636002724013785	26.2323020289435	26.984967245415586	23.146728001627125
48	23.488251249688822	26.308562649475046	26.930623703045768	23.27256239779036
49	23.315271684124742	26.422691243101777	26.956579183712453	23.305457889061028
50	23.54022183310642	26.197590051506726	26.909175651946622	23.353012463440233
51	23.439647326627227	26.156828419923745	26.913345223036877	23.490179030412154
52	23.465511386764764	26.16699994749282	26.992209341246326	23.375279324496095
53	23.551502331442105	25.96590260700743	26.850431999760875	23.632163061789583
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	62.5
2	61.0
3	83.0
4	105.0
5	130.5
6	156.0
7	213.0
8	370.5
9	471.0
10	793.0
11	1115.0
12	1895.5
13	2676.0
14	4714.0
15	6752.0
16	11490.0
17	16228.0
18	26537.0
19	36846.0
20	53216.0
21	69586.0
22	200803.0
23	332020.0
24	266178.5
25	225068.5
26	249800.0
27	286162.0
28	322524.0
29	373228.0
30	423932.0
31	484333.5
32	544735.0
33	618327.5
34	691920.0
35	780784.0
36	869648.0
37	968335.5
38	1067023.0
39	1179342.5
40	1291662.0
41	1401787.5
42	1616281.5
43	1720650.0
44	1787353.5
45	1854057.0
46	1891804.0
47	1929551.0
48	1958789.5
49	1988028.0
50	1962839.5
51	1937651.0
52	1886846.0
53	1836041.0
54	1732892.5
55	1629744.0
56	1516595.5
57	1403447.0
58	1246386.5
59	936787.0
60	784248.0
61	665549.0
62	546850.0
63	447623.0
64	348396.0
65	279411.0
66	210426.0
67	164949.0
68	119472.0
69	90988.5
70	62505.0
71	46525.5
72	30546.0
73	22660.5
74	14775.0
75	11176.5
76	5180.5
77	2783.0
78	1991.5
79	1200.0
80	959.5
81	719.0
82	510.5
83	302.0
84	226.5
85	151.0
86	115.5
87	80.0
88	56.0
89	32.0
90	26.0
91	20.0
92	20.0
93	14.5
94	9.0
95	9.0
96	9.0
97	6.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.5158463E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.062274341816746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.12048305769794	33.421458280738555
2	16.822440777953958	16.506944090938845
3	6.206303692037901	9.134861231621809
4	2.970648178979034	5.829870237203508
5	1.7017188729629793	4.1745099098978455
6	1.0023570113289335	2.950674880695819
7	0.6485420754142901	2.2273264458330977
8	0.4469541787471834	1.754287082873257
9	0.33136223406813875	1.4631646350931486
>10	1.623696219396039	14.340263072815873
>50	0.0862484402179386	2.8450847152231664
>100	0.03753015642843655	3.4197339073859543
>500	0.0012542531923374093	0.3941297763587409
>1k	3.7072056994066004E-4	0.3159461719888188
>5k	6.309170361420531E-5	0.2190365161089036
>10k+	2.7039301548773127E-5	1.0027090452225518
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	196170	0.7797376175166185	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.008502109210725632	7.949611230224994E-6	0.0	3.974805615112497E-6	0.0
2	0.011216901445847466	7.949611230224994E-6	0.0	3.974805615112497E-6	0.0
3	0.011228825862692804	7.949611230224994E-6	0.0	3.974805615112497E-6	3.974805615112497E-6
4	0.011304347169379942	7.949611230224994E-6	0.0	7.949611230224994E-6	3.974805615112497E-6
5	0.011344095225531066	2.782363930578748E-5	0.0	7.949611230224994E-6	3.974805615112497E-6
6	0.011817097093729453	2.782363930578748E-5	0.0	7.949611230224994E-6	3.974805615112497E-6
7	0.01232189740684874	2.782363930578748E-5	0.0	1.1924416845337492E-5	1.1924416845337492E-5
8	0.013208279059018828	2.782363930578748E-5	0.0	1.1924416845337492E-5	1.1924416845337492E-5
9	0.01634440068934259	3.179844492089998E-5	0.0	1.9874028075562484E-5	1.1924416845337492E-5
10	0.020573593863822286	3.577325053601247E-5	0.0	1.9874028075562484E-5	1.1924416845337492E-5
11	0.026337062005735407	3.577325053601247E-5	0.0	1.9874028075562484E-5	1.1924416845337492E-5
12	0.03287561724259546	3.577325053601247E-5	0.0	1.9874028075562484E-5	1.589922246044999E-5
13	0.04327768353734487	3.577325053601247E-5	0.0	1.9874028075562484E-5	1.589922246044999E-5
14	0.06041704534970996	3.577325053601247E-5	0.0	1.9874028075562484E-5	1.589922246044999E-5
15	0.08973918637239485	3.577325053601247E-5	0.0	2.3848833690674983E-5	1.589922246044999E-5
16	0.11327798522509105	3.577325053601247E-5	0.0	2.782363930578748E-5	1.589922246044999E-5
17	0.12308383067757359	3.577325053601247E-5	0.0	3.179844492089998E-5	1.589922246044999E-5
18	0.12829480083898606	3.577325053601247E-5	0.0	3.577325053601247E-5	1.589922246044999E-5
19	0.13250411998539022	3.974805615112497E-5	0.0	3.577325053601247E-5	1.589922246044999E-5
20	0.13432855576272684	3.974805615112497E-5	0.0	3.577325053601247E-5	1.589922246044999E-5
21	0.13545740055741878	3.974805615112497E-5	0.0	3.974805615112497E-5	1.589922246044999E-5
22	0.136419303516276	4.7697667381349966E-5	0.0	3.974805615112497E-5	1.589922246044999E-5
23	0.13736133244705767	5.564727861157496E-5	0.0	3.974805615112497E-5	1.589922246044999E-5
24	0.13821194084869176	6.359688984179995E-5	0.0	3.974805615112497E-5	1.589922246044999E-5
25	0.13903870041663516	6.359688984179995E-5	0.0	4.372286176623747E-5	1.589922246044999E-5
26	0.13996483012495636	6.359688984179995E-5	0.0	4.372286176623747E-5	1.9874028075562484E-5
27	0.14087903541643224	6.359688984179995E-5	0.0	4.372286176623747E-5	1.9874028075562484E-5
28	0.14297773278121165	6.359688984179995E-5	0.0	4.372286176623747E-5	1.9874028075562484E-5
29	0.14379256793230968	6.359688984179995E-5	0.0	4.7697667381349966E-5	1.9874028075562484E-5
30	0.14608603077222962	6.359688984179995E-5	0.0	4.7697667381349966E-5	2.3848833690674983E-5
31	0.17825413261533504	6.359688984179995E-5	0.0	5.167247299646246E-5	2.3848833690674983E-5
32	0.17895767320920997	6.359688984179995E-5	0.0	5.167247299646246E-5	2.3848833690674983E-5
33	0.1793869522156421	6.359688984179995E-5	0.0	5.167247299646246E-5	3.577325053601247E-5
34	0.17961749094131862	6.359688984179995E-5	0.0	5.564727861157496E-5	3.577325053601247E-5
35	0.17990765175122184	6.757169545691246E-5	0.0	6.757169545691246E-5	3.974805615112497E-5
36	0.1802455102285064	7.552130668713745E-5	0.0	6.757169545691246E-5	4.372286176623747E-5
37	0.18108419421329514	7.552130668713745E-5	0.0	7.949611230224994E-5	4.372286176623747E-5
38	0.1814220526905797	7.552130668713745E-5	0.0	8.347091791736244E-5	4.372286176623747E-5
39	0.1817320875285585	7.552130668713745E-5	0.0	9.142052914758743E-5	4.372286176623747E-5
40	0.181998399504771	7.949611230224994E-5	0.0	9.539533476269993E-5	4.372286176623747E-5
41	0.18235215720451603	7.949611230224994E-5	0.0	9.539533476269993E-5	4.372286176623747E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	162075	0.0	43.031498	12
ATCAACG	161995	0.0	43.00923	11
AAGCAGT	161420	0.0	42.95716	1
CAACGCA	162535	0.0	42.927063	13
AGTGGTA	162700	0.0	42.8142	5
GTGGTAT	163005	0.0	42.703815	6
GGTATCA	163160	0.0	42.669006	8
GTATCAA	163260	0.0	42.621284	9
AACGCAG	164170	0.0	42.499546	14
TATCAAC	163780	0.0	42.49887	10
ACGCAGA	164495	0.0	42.407005	15
CGCAGAG	165490	0.0	42.174755	16
AGAGTAC	164475	0.0	41.993526	19
TGGTATC	166325	0.0	41.86271	7
CAGTGGT	168755	0.0	41.276615	4
CAGAGTA	168460	0.0	41.13965	18
GTACATG	134350	0.0	40.877934	22
ACATGGG	133440	0.0	40.73052	24
TACATGG	134835	0.0	40.66641	23
GCAGTGG	170815	0.0	40.642628	3
>>END_MODULE