FastQCFastQC Report
Thu 26 May 2016
SRR805289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19764756
Sequences flagged as poor quality0
Sequence length53
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT13650.014.805860512
TCAACGC170650.014.01875111
CAACGCA174400.013.64994212
ATCAACG176000.013.61931710
TATGCCG15150.013.495048516
AGTGGTA179300.013.4079754
AACGCAG181300.013.26006513
GTGGTAT184550.013.0774855
ACGCAGA183850.013.06336614
AAGCAGT128950.013.0484681
GATCGGA15500.013.0387091
GCTCGTA16100.012.99068311
GGTATCA187100.012.761097
CGCAGAG190900.012.64248315
GTATCAA190800.012.5382598
TATCAAC192250.012.4925879
TGGTATC204350.011.9138736
TCGTATG17000.011.88823513
AGAGTAC200300.011.84972518
AGCAGTG215050.011.8237611
Produced by FastQC (version 0.11.5)