##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805289_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19764756
Sequences flagged as poor quality	0
Sequence length	53
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.74297299698514	37.0	35.0	37.0	28.0	37.0
2	35.84075356154156	37.0	35.0	37.0	33.0	37.0
3	37.98310452200877	39.0	37.0	39.0	35.0	39.0
4	38.14897937520706	39.0	39.0	39.0	37.0	39.0
5	38.140735812777045	39.0	39.0	39.0	37.0	39.0
6	38.10885902158367	39.0	39.0	39.0	37.0	39.0
7	38.116351803179356	39.0	39.0	39.0	37.0	39.0
8	39.13957460441201	40.0	40.0	40.0	37.0	40.0
9	39.144241244364466	40.0	40.0	40.0	37.0	40.0
10	39.12578723461094	40.0	40.0	40.0	37.0	40.0
11	39.16754590848478	40.0	40.0	40.0	38.0	40.0
12	39.16029395961174	40.0	40.0	40.0	38.0	40.0
13	39.190390106510804	40.0	40.0	40.0	38.0	40.0
14	39.131502205238455	40.0	40.0	40.0	37.0	40.0
15	39.11995088631502	40.0	40.0	40.0	37.0	40.0
16	39.088054818384805	40.0	40.0	40.0	37.0	40.0
17	39.053399191975856	40.0	40.0	40.0	37.0	40.0
18	39.016852927503884	40.0	40.0	40.0	37.0	40.0
19	39.00299654597305	40.0	40.0	40.0	37.0	40.0
20	38.9307733928008	40.0	40.0	40.0	37.0	40.0
21	38.92705050343146	40.0	40.0	40.0	37.0	40.0
22	38.8732935028391	40.0	40.0	40.0	37.0	40.0
23	38.885916830948986	40.0	40.0	40.0	37.0	40.0
24	38.89149023645928	40.0	40.0	40.0	37.0	40.0
25	38.87306764626894	40.0	40.0	40.0	37.0	40.0
26	38.854046819500326	40.0	40.0	40.0	37.0	40.0
27	38.838039133900764	40.0	40.0	40.0	37.0	40.0
28	38.81775039368055	40.0	40.0	40.0	37.0	40.0
29	38.77728184451151	40.0	39.0	40.0	36.0	40.0
30	38.72351629334559	40.0	39.0	40.0	36.0	40.0
31	38.703928700156986	40.0	39.0	40.0	36.0	40.0
32	38.676945316198186	40.0	39.0	40.0	36.0	40.0
33	38.33287747139403	40.0	39.0	40.0	35.0	40.0
34	38.47149178062203	40.0	39.0	40.0	35.0	40.0
35	38.54120855324498	40.0	39.0	40.0	35.0	40.0
36	38.51271753620434	40.0	39.0	40.0	35.0	40.0
37	38.49383093826203	40.0	39.0	40.0	35.0	40.0
38	38.40324196261264	40.0	39.0	40.0	35.0	40.0
39	38.400889795958015	40.0	38.0	40.0	35.0	40.0
40	38.35174747414033	40.0	38.0	40.0	35.0	40.0
41	38.32693315313379	40.0	38.0	40.0	35.0	40.0
42	38.26476856076543	40.0	38.0	40.0	35.0	40.0
43	38.18856200400349	40.0	38.0	40.0	35.0	40.0
44	38.0584183786534	40.0	38.0	40.0	34.0	40.0
45	37.12565583911079	40.0	37.0	40.0	32.0	40.0
46	37.57497132775128	40.0	37.0	40.0	33.0	40.0
47	37.69372078258897	40.0	37.0	40.0	33.0	40.0
48	37.59162728849271	40.0	37.0	40.0	33.0	40.0
49	37.69641117755261	40.0	38.0	40.0	34.0	40.0
50	37.709731554490226	40.0	38.0	40.0	34.0	40.0
51	37.788348209307514	40.0	38.0	40.0	34.0	40.0
52	37.74894812766725	40.0	37.0	40.0	34.0	40.0
53	37.2983323953	40.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	11.0
12	28.0
13	62.0
14	214.0
15	519.0
16	1208.0
17	2287.0
18	3461.0
19	5135.0
20	7095.0
21	9934.0
22	13769.0
23	18803.0
24	26169.0
25	35618.0
26	48859.0
27	64986.0
28	85202.0
29	109582.0
30	139820.0
31	174187.0
32	219551.0
33	273695.0
34	353327.0
35	481114.0
36	699463.0
37	1205172.0
38	3147059.0
39	1.2638419E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.146249212487117	31.414245640067602	20.71873287987972	27.720772267565557
2	23.533055505466397	23.853646359206255	31.4446785986126	21.168619536714747
3	18.9027833179423	27.104893174497068	28.82593136995974	25.16639213760089
4	20.165444997145425	29.729094555986425	28.55224724251592	21.553213204352232
5	24.27353011592959	28.698563240547976	26.590452217067593	20.43745442645485
6	22.51730808111165	27.3323181930503	28.725773290598678	21.42460043523937
7	22.76841667056249	28.340471291423984	27.69129555659579	21.199816481417734
8	21.140615143440172	26.9138207423355	29.231258913593468	22.71430520063086
9	21.079577202976854	26.705955793231144	30.04807142572365	22.166395578068357
10	21.6968881376527	27.65792302217138	28.56548798275071	22.07970085742521
11	21.63373532159972	27.78613103040584	28.358483150512964	22.221650497481477
12	21.724973483102954	27.681080403927073	28.001094473415204	22.59285163955477
13	21.991817151701746	27.900809906279644	27.59126902452021	22.516103917498402
14	22.199292518460638	27.892831057464107	27.48667375402965	22.421202670045613
15	22.14549473820977	27.68717205514705	27.42459861381542	22.74273459282776
16	22.556074054240792	27.64627602789531	27.26809276066955	22.52955715719435
17	22.820929335024424	27.409379604787432	27.20484887341893	22.564842186769216
18	22.403241406066435	27.655130172110397	27.148541575721957	22.79308684610121
19	22.83257126978952	27.570378303683583	27.11697022720645	22.480080199320447
20	22.75727562738442	27.527013235073582	27.240199676636536	22.475511460905462
21	22.63990509166923	27.631421303657884	27.249271379823764	22.47940222484912
22	22.61101528397315	27.401906707069895	27.42589890813729	22.56117910081966
23	22.41730684659097	27.573666985820623	27.579121138657115	22.429905028931294
24	22.403185751445655	27.3941454172265	27.48041513894733	22.722253692380516
25	22.469859987140747	27.461558341524682	27.44515034741638	22.62343132391819
26	22.491120052278916	27.30889265721267	27.790047091904395	22.40994019860402
27	22.61148581849429	27.146755568346	27.70223421933466	22.53952439382505
28	22.761338414701402	27.2798713022311	27.48982582937022	22.46896445369728
29	22.748208983708174	27.278743031282556	27.544908725410018	22.42813925959926
30	22.63562980489109	27.227095543198203	27.477424967958118	22.659849683952586
31	22.69407727573262	27.342255072615114	27.369981192785787	22.59368645886648
32	22.655154457763103	27.356386286782392	27.43696405865066	22.55149519680385
33	22.798298142410665	27.229620239177247	27.378137124485626	22.59394449392646
34	22.733121521965664	27.373037137417732	27.265998123123808	22.627843217492792
35	22.67437048046533	27.366080309820166	27.383955562112682	22.57559364760182
36	22.663725269363304	27.29592007105982	27.372819578445593	22.66753508113128
37	22.686826996498212	27.40514479409713	27.246918707218036	22.66110950218662
38	22.702359695206965	27.35195921467485	27.366783581846395	22.578897508271794
39	22.66417050632955	27.280048385115403	27.39780850317606	22.65797260537899
40	22.66328003239706	27.36728447343342	27.346707442277555	22.622728051891965
41	22.67018626488483	27.31113908008781	27.41958463843419	22.599090016593173
42	22.66536455092084	27.238130336645693	27.425357540462425	22.671147571971037
43	22.681995163512266	27.336998240706844	27.3098489047879	22.671157690993
44	22.705248675976573	27.26246152494875	27.4164376226046	22.615852176470078
45	22.7427497713607	27.28353438818066	27.23897527497936	22.73474056547928
46	22.706928433621947	27.257609453918885	27.33451908032662	22.70094303213255
47	22.694583226830627	27.2570478482001	27.386095735257243	22.66227318971203
48	22.648738997840397	27.211492011335732	27.395673389542473	22.74409560128139
49	22.7532482566443	27.31754948049953	27.240402057075734	22.688800205780428
50	22.766883638735536	27.307278673210035	27.312834016266123	22.61300367178831
51	22.717092991180866	27.200856919255667	27.339168770917283	22.74288131864618
52	22.777797003919503	27.297534055062457	27.17963227069436	22.74503667032368
53	22.91847670671978	27.23150237726183	27.26106004040728	22.588960875611114
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1266.0
1	1162.0
2	1058.0
3	1058.0
4	1058.0
5	1162.5
6	1267.0
7	1459.0
8	2203.0
9	2755.0
10	3892.5
11	5030.0
12	7454.5
13	9879.0
14	15125.5
15	20372.0
16	30442.0
17	40512.0
18	60095.0
19	79678.0
20	102924.5
21	126171.0
22	168388.5
23	210606.0
24	234934.5
25	297489.0
26	335715.0
27	378021.5
28	420328.0
29	470172.0
30	520016.0
31	573027.0
32	626038.0
33	682532.5
34	739027.0
35	808079.0
36	877131.0
37	941686.0
38	1006241.0
39	1073292.5
40	1140344.0
41	1196056.0
42	1299644.5
43	1347521.0
44	1365120.5
45	1382720.0
46	1382248.0
47	1381776.0
48	1365388.0
49	1349000.0
50	1323762.5
51	1298525.0
52	1250566.5
53	1202608.0
54	1131742.0
55	1060876.0
56	980491.0
57	900106.0
58	803744.5
59	614608.0
60	521833.0
61	443767.0
62	365701.0
63	304666.0
64	243631.0
65	196744.0
66	149857.0
67	117942.0
68	86027.0
69	65640.0
70	45253.0
71	34179.0
72	23105.0
73	17077.0
74	11049.0
75	8213.5
76	3872.0
77	2366.0
78	1775.0
79	1184.0
80	944.5
81	705.0
82	556.5
83	408.0
84	306.0
85	204.0
86	168.5
87	133.0
88	109.0
89	85.0
90	70.0
91	55.0
92	47.0
93	30.0
94	21.0
95	19.5
96	18.0
97	14.5
98	11.0
99	7.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.9764756E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.83218359375573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.74874757338782	33.92984591303706
2	18.172972410128374	18.475437399918064
3	6.472814181148342	9.870818364731946
4	3.0020923814933638	6.10411644405944
5	1.5899803139941855	4.041108561570491
6	0.9891677142580735	3.0168932913709257
7	0.6304937040513555	2.2434560203331912
8	0.4280463785770577	1.740682568197701
9	0.304476365355754	1.3929478653350822
>10	1.5490130612995088	14.287970003204034
>50	0.08994468005518075	3.0447098398630428
>100	0.02214980156664956	1.695227403534247
>500	6.158661852264792E-5	0.0230231433593737
>1k	1.9924036697842462E-5	0.029927716018790306
>5k	9.962014611624038E-6	0.05026943017251276
>10k+	9.962014611624038E-6	0.05356603529413115
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7708288430173385E-4	0.0	0.0	2.0238043920198155E-5	5.059510980049539E-6
2	1.7708288430173385E-4	5.059510980049539E-6	0.0	3.541657686034677E-5	5.059510980049539E-6
3	1.7708288430173385E-4	1.0119021960099077E-5	0.0	5.059510980049539E-5	5.059510980049539E-6
4	1.7708288430173385E-4	1.0119021960099077E-5	0.0	6.5773642740644E-5	5.059510980049539E-6
5	1.8720190626183292E-4	1.0119021960099077E-5	0.0	7.589266470074308E-5	5.059510980049539E-6
6	1.9226141724188248E-4	1.0119021960099077E-5	0.0	7.589266470074308E-5	5.059510980049539E-6
7	1.9226141724188248E-4	1.0119021960099077E-5	0.0	8.601168666084216E-5	5.059510980049539E-6
8	1.9226141724188248E-4	1.0119021960099077E-5	0.0	8.601168666084216E-5	5.059510980049539E-6
9	1.9226141724188248E-4	1.0119021960099077E-5	0.0	9.613070862094124E-5	5.059510980049539E-6
10	1.97320928221932E-4	1.0119021960099077E-5	0.0	1.0119021960099078E-4	5.059510980049539E-6
11	2.1249946116208064E-4	1.0119021960099077E-5	0.0	1.0624973058104032E-4	5.059510980049539E-6
12	2.1249946116208064E-4	1.0119021960099077E-5	0.0	1.1636875254113938E-4	5.059510980049539E-6
13	2.2261848312217972E-4	1.0119021960099077E-5	0.0	1.2648777450123848E-4	5.059510980049539E-6
14	2.2767799410222924E-4	1.0119021960099077E-5	0.0	1.2648777450123848E-4	5.059510980049539E-6
15	2.4791603802242737E-4	1.0119021960099077E-5	0.0	1.31547285481288E-4	1.0119021960099077E-5
16	2.63094570962576E-4	2.0238043920198155E-5	0.0	1.6190435136158524E-4	1.0119021960099077E-5
17	2.732135929226751E-4	2.0238043920198155E-5	0.0	1.6190435136158524E-4	1.0119021960099077E-5
18	2.8333261488277416E-4	2.0238043920198155E-5	0.0	1.6696386234163477E-4	1.0119021960099077E-5
19	2.9345163684287327E-4	2.0238043920198155E-5	0.0	1.7202337332168432E-4	1.5178532940148615E-5
20	3.2886821370322E-4	2.0238043920198155E-5	0.0	1.7202337332168432E-4	1.5178532940148615E-5
21	3.642847905635668E-4	2.0238043920198155E-5	0.0	1.821423952817834E-4	1.5178532940148615E-5
22	3.94641856443864E-4	2.0238043920198155E-5	0.0	1.821423952817834E-4	1.5178532940148615E-5
23	4.351179442842603E-4	2.0238043920198155E-5	0.0	1.821423952817834E-4	2.0238043920198155E-5
24	4.8065354310470617E-4	2.0238043920198155E-5	0.0	1.821423952817834E-4	3.035706588029723E-5
25	5.16070119965053E-4	2.0238043920198155E-5	0.0	1.821423952817834E-4	3.035706588029723E-5
26	5.869032736857465E-4	2.0238043920198155E-5	0.0	1.9226141724188248E-4	3.035706588029723E-5
27	6.374983834862419E-4	2.0238043920198155E-5	0.0	2.0238043920198156E-4	3.035706588029723E-5
28	7.13391048186985E-4	2.0238043920198155E-5	0.0	2.0238043920198156E-4	3.035706588029723E-5
29	7.589266470074307E-4	2.5297554900247695E-5	0.0	2.1249946116208064E-4	3.035706588029723E-5
30	8.297598007281243E-4	2.5297554900247695E-5	0.0	2.1755897214213016E-4	3.035706588029723E-5
31	9.309500203291151E-4	2.5297554900247695E-5	0.0	2.1755897214213016E-4	3.035706588029723E-5
32	0.001032140239930106	2.5297554900247695E-5	0.0	2.2261848312217972E-4	3.541657686034677E-5
33	0.0012041636132517901	2.5297554900247695E-5	0.0	2.2261848312217972E-4	3.541657686034677E-5
34	0.0013255918767729792	2.5297554900247695E-5	0.0	2.2767799410222924E-4	3.541657686034677E-5
35	0.001436901118334069	3.035706588029723E-5	0.0	2.2767799410222924E-4	3.541657686034677E-5
36	0.0016291625355759514	3.035706588029723E-5	0.0	2.4791603802242737E-4	3.541657686034677E-5
37	0.001831542974777933	3.035706588029723E-5	0.0	2.4791603802242737E-4	3.541657686034677E-5
38	0.002064280479860212	3.035706588029723E-5	0.0	2.5297554900247695E-4	3.541657686034677E-5
39	0.002261601408082144	3.035706588029723E-5	0.0	2.580350599825265E-4	3.541657686034677E-5
40	0.0024639818472841253	3.035706588029723E-5	0.0	2.63094570962576E-4	3.541657686034677E-5
41	0.002732135929226751	3.035706588029723E-5	0.0	2.63094570962576E-4	3.541657686034677E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAT	1365	0.0	14.8058605	12
TCAACGC	17065	0.0	14.018751	11
CAACGCA	17440	0.0	13.649942	12
ATCAACG	17600	0.0	13.619317	10
TATGCCG	1515	0.0	13.4950485	16
AGTGGTA	17930	0.0	13.407975	4
AACGCAG	18130	0.0	13.260065	13
GTGGTAT	18455	0.0	13.077485	5
ACGCAGA	18385	0.0	13.063366	14
AAGCAGT	12895	0.0	13.048468	1
GATCGGA	1550	0.0	13.038709	1
GCTCGTA	1610	0.0	12.990683	11
GGTATCA	18710	0.0	12.76109	7
CGCAGAG	19090	0.0	12.642483	15
GTATCAA	19080	0.0	12.538259	8
TATCAAC	19225	0.0	12.492587	9
TGGTATC	20435	0.0	11.913873	6
TCGTATG	1700	0.0	11.888235	13
AGAGTAC	20030	0.0	11.849725	18
AGCAGTG	21505	0.0	11.823761	1
>>END_MODULE