FastQCFastQC Report
Thu 26 May 2016
SRR805288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13488030
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT200820.14888756920024643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT175700.029.358281
TCAACGC188400.027.34182512
ATCAACG190450.027.08453811
CAACGCA190200.027.0583613
AGTGGTA194000.026.4677835
GGTATCA194800.026.2746418
AACGCAG197500.026.14151814
ACGCAGA198850.025.88131515
GTATCAA198550.025.8257359
GTGGTAT199900.025.639576
TATCAAC201200.025.53230710
CGCAGAG204200.025.20323216
CTCGTAT13900.024.68345312
TGGTATC208700.024.5359367
AGAGTAC207900.024.4042819
GATCGGA14150.024.247351
CAGTGGT230050.022.4018694
GCTCGTA15400.022.27922211
CAGAGTA229400.022.27070618
GCAGTGG235800.022.0050893
Produced by FastQC (version 0.11.5)