##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805288_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13488030
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.593312737293736	37.0	35.0	37.0	28.0	37.0
2	35.761124345067444	37.0	35.0	37.0	33.0	37.0
3	37.89233765049455	39.0	38.0	39.0	35.0	39.0
4	38.08634359502462	39.0	39.0	39.0	37.0	39.0
5	38.11232707815745	39.0	39.0	39.0	37.0	39.0
6	38.09937018230238	39.0	39.0	39.0	37.0	39.0
7	38.10469823984674	39.0	39.0	39.0	37.0	39.0
8	39.036577543199414	40.0	40.0	40.0	37.0	40.0
9	39.05335723600852	40.0	40.0	40.0	37.0	40.0
10	39.01274018518642	40.0	40.0	40.0	37.0	40.0
11	39.09555376137212	40.0	40.0	40.0	37.0	40.0
12	39.10774827754683	40.0	40.0	40.0	37.0	40.0
13	39.13604040026601	40.0	40.0	40.0	38.0	40.0
14	39.08796888796955	40.0	40.0	40.0	37.0	40.0
15	39.063404144267174	40.0	40.0	40.0	37.0	40.0
16	39.04135874549508	40.0	40.0	40.0	37.0	40.0
17	38.9808175841839	40.0	40.0	40.0	37.0	40.0
18	38.93645469353197	40.0	40.0	40.0	37.0	40.0
19	38.91517812460381	40.0	40.0	40.0	37.0	40.0
20	38.86408319079955	40.0	40.0	40.0	37.0	40.0
21	38.845829598540334	40.0	40.0	40.0	37.0	40.0
22	38.79584527911044	40.0	40.0	40.0	37.0	40.0
23	38.785031246223504	40.0	40.0	40.0	36.0	40.0
24	38.806335988279976	40.0	40.0	40.0	37.0	40.0
25	38.78585605162503	40.0	40.0	40.0	36.0	40.0
26	38.79149230836527	40.0	40.0	40.0	36.0	40.0
27	38.78218375848808	40.0	40.0	40.0	36.0	40.0
28	38.75578086644232	40.0	40.0	40.0	36.0	40.0
29	38.70571365870331	40.0	40.0	40.0	36.0	40.0
30	38.653698130861216	40.0	39.0	40.0	36.0	40.0
31	38.65519923962209	40.0	39.0	40.0	36.0	40.0
32	38.64837251993063	40.0	39.0	40.0	36.0	40.0
33	38.201321764557164	40.0	39.0	40.0	35.0	40.0
34	38.41482247592866	40.0	39.0	40.0	35.0	40.0
35	38.48675499683794	40.0	39.0	40.0	35.0	40.0
36	38.464158813407145	40.0	39.0	40.0	35.0	40.0
37	38.430407331537666	40.0	39.0	40.0	35.0	40.0
38	38.308669538842956	40.0	38.0	40.0	35.0	40.0
39	38.306552773088434	40.0	38.0	40.0	35.0	40.0
40	38.2906528974209	40.0	38.0	40.0	35.0	40.0
41	38.26149660106035	40.0	38.0	40.0	35.0	40.0
42	38.1619760632205	40.0	38.0	40.0	35.0	40.0
43	38.093990745868744	40.0	38.0	40.0	35.0	40.0
44	37.99410499531807	40.0	38.0	40.0	34.0	40.0
45	37.52713361402666	40.0	38.0	40.0	33.0	40.0
46	37.70455515001079	40.0	38.0	40.0	33.0	40.0
47	37.74644948150323	40.0	38.0	40.0	34.0	40.0
48	37.67470334807974	40.0	37.0	40.0	34.0	40.0
49	37.67836274088951	40.0	37.0	40.0	34.0	40.0
50	37.635303079841904	40.0	37.0	40.0	34.0	40.0
51	37.649486099897466	40.0	37.0	40.0	34.0	40.0
52	37.56467764380714	40.0	37.0	40.0	34.0	40.0
53	37.16921492612339	39.0	36.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	17.0
11	31.0
12	74.0
13	146.0
14	290.0
15	634.0
16	1296.0
17	2042.0
18	3107.0
19	4288.0
20	5836.0
21	8093.0
22	11295.0
23	15704.0
24	21856.0
25	29514.0
26	39621.0
27	52258.0
28	68135.0
29	85824.0
30	107366.0
31	132870.0
32	161071.0
33	196183.0
34	246391.0
35	330279.0
36	469507.0
37	798522.0
38	1978513.0
39	8717262.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.717598492885912	32.70836437937935	22.883304678296238	26.690732449438503
2	27.889699237027198	25.62987330247634	25.830035965222496	20.650391495273958
3	21.876901222787907	25.115068694242225	26.452239504212255	26.55579057875761
4	20.949642015920784	30.386438938822053	24.279653885704583	24.38426515955258
5	25.474268666365656	28.739993905707507	24.0262662523734	21.759471175553436
6	24.853955692565926	26.293231850759526	26.225223401786625	22.627589054887924
7	24.037735681192878	27.301147758419873	26.568868841483894	22.092247718903355
8	22.80833450103536	26.344217799041076	27.167859205532608	23.679588494390952
9	22.692253798367886	25.89415948807943	28.083367252297037	23.33021946125565
10	22.492498904584288	26.95277961273811	27.462134944836276	23.09258653784133
11	22.42642550468823	27.59291757209911	26.85956362789822	23.121093295314438
12	22.390897707078054	26.738115202887304	27.2598444695037	23.611142620530945
13	22.630028254682113	27.043652779538597	26.428603732346385	23.897715233432905
14	22.950008266588966	27.648633640346294	26.167601940387144	23.233756152677596
15	22.875290164686763	27.42056475259916	26.130732212191106	23.573412870522976
16	23.253892525446638	26.717697098835043	26.121079208750274	23.907331166968046
17	24.10945112073446	26.47190879616964	26.02030096315029	23.39833911994561
18	23.046338123506548	26.700541146483214	26.01012156704871	24.242999162961528
19	23.47777251385117	27.246973798249265	26.05087622136072	23.22437746653885
20	24.071254289914837	26.58381542745679	26.091482596049982	23.253447686578397
21	23.333748516277026	27.232308943559584	26.124763957375542	23.309178582787847
22	23.95307543058549	26.46102507186001	26.369306711209866	23.216592786344634
23	23.170559377462833	26.637611274589396	27.074072344145144	23.11775700380263
24	23.058178251382895	27.096054798217384	26.27075265995108	23.575014290448642
25	23.09389881250264	26.639479597836008	26.32303605493167	23.943585534729685
26	23.184786807265407	26.92677878088943	26.781205261257572	23.107229150587592
27	23.331309316482837	26.237901309531487	27.11938659685662	23.311402777129054
28	23.907561000383303	26.385728679429093	26.537685636820203	23.1690246833674
29	24.0214619925964	26.357911422201756	26.48159145553502	23.139035129666823
30	23.862187435822726	26.28426834756447	26.463026846767097	23.390517369845707
31	23.59613672270895	26.53198428532558	26.50108281194511	23.37079618002036
32	23.570944014804237	26.598205964844386	26.56596997485919	23.26488004549219
33	23.726452269160138	26.380753898085935	26.517727199598458	23.375066633155473
34	23.51301116619699	26.66507266072214	26.463634793220358	23.358281379860514
35	23.50772499764606	26.60367006894261	26.51187756848109	23.37672736493024
36	23.4684679675238	26.51247068697208	26.534282619478162	23.484778726025965
37	23.43480849316023	26.7086965257343	26.491192561107884	23.365302419997583
38	23.57150006338954	26.574384843450083	26.543409230258234	23.310705862902143
39	23.47695697592606	26.50553861460866	26.566155324387626	23.45134908507766
40	23.43111633055383	26.627320668770754	26.547101392864636	23.394461607810776
41	23.49645574631729	26.52591964875523	26.604685784358427	23.372938820569054
42	23.50366954996393	26.461010243897736	26.60623530641613	23.4290848997222
43	23.46525029971019	26.592519441312035	26.566956034350458	23.37527422462732
44	23.51748179682281	26.471197053980454	26.609475216173156	23.401845933023576
45	23.529396064510532	26.363316214450887	26.506057593288272	23.60123012775031
46	23.47869926149334	26.517823581353245	26.544424945674056	23.459052211479364
47	23.55306149230095	26.47010719875327	26.583348346645135	23.393482962300645
48	23.51687385036955	26.399429716570914	26.539783793482073	23.543912639577464
49	23.48946436210477	26.568483314464753	26.466392794203454	23.475659529227023
50	23.59736002959661	26.517386156466138	26.508652486686344	23.376601327250903
51	23.550770572129508	26.383393275370832	26.52023312522288	23.54560302727678
52	23.516577291124054	26.53579507163018	26.45023031532403	23.497397321921735
53	23.708414053052966	26.47274657603816	26.451453622211694	23.367385748697178
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	169.0
1	149.0
2	129.0
3	129.5
4	130.0
5	148.5
6	167.0
7	213.5
8	384.5
9	509.0
10	815.5
11	1122.0
12	1821.0
13	2520.0
14	4122.5
15	5725.0
16	9063.5
17	12402.0
18	19619.5
19	26837.0
20	36372.5
21	45908.0
22	73486.0
23	101064.0
24	106570.0
25	130737.0
26	149398.0
27	172974.0
28	196550.0
29	226360.5
30	256171.0
31	290473.5
32	324776.0
33	363948.0
34	403120.0
35	452091.0
36	501062.0
37	551411.0
38	601760.0
39	656867.5
40	711975.0
41	761672.0
42	859592.0
43	907815.0
44	936819.0
45	965823.0
46	982142.5
47	998462.0
48	1002006.5
49	1005551.0
50	1000083.0
51	994615.0
52	970282.0
53	945949.0
54	899335.0
55	852721.0
56	795926.0
57	739131.0
58	663176.5
59	512370.5
60	437519.0
61	374238.0
62	310957.0
63	258346.5
64	205736.0
65	166420.5
66	127105.0
67	99788.0
68	72471.0
69	55007.0
70	37543.0
71	27892.0
72	18241.0
73	13337.5
74	8434.0
75	6217.5
76	2805.5
77	1610.0
78	1181.0
79	752.0
80	605.0
81	458.0
82	353.0
83	248.0
84	212.5
85	177.0
86	139.5
87	102.0
88	77.5
89	53.0
90	49.5
91	46.0
92	41.5
93	28.5
94	20.0
95	21.5
96	23.0
97	15.5
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.348803E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.570289467830406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.18917353682895	47.52559758445153
2	13.01621152801697	16.028238231090324
3	4.264002810105075	7.876076619294355
4	1.9483494387571243	4.798417557150443
5	0.9906957754130457	3.0498712833368966
6	0.5858445118080235	2.164236970509588
7	0.3991101949154624	1.7201331161355065
8	0.2805966763187291	1.3821134867724212
9	0.21069639976521287	1.1675374491036485
>10	1.0622581016269812	11.367339265831673
>50	0.041918090840967245	1.7303497193160253
>100	0.011033889346958608	0.9677580654363074
>500	8.492377523502212E-5	0.03809552561842157
>1k	1.20612406042925E-5	0.035103367341673436
>5k	0.0	0.0
>10k+	1.2061240604292497E-5	0.14913175861127265
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20082	0.14888756920024643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.520753586698725E-4	0.0	0.0	0.0	0.0
2	2.520753586698725E-4	0.0	0.0	0.0	0.0
3	2.520753586698725E-4	0.0	0.0	0.0	0.0
4	2.520753586698725E-4	0.0	0.0	0.0	0.0
5	2.5948933980722165E-4	0.0	0.0	7.41398113734919E-6	0.0
6	2.5948933980722165E-4	0.0	0.0	7.41398113734919E-6	0.0
7	2.5948933980722165E-4	0.0	0.0	7.41398113734919E-6	0.0
8	2.5948933980722165E-4	0.0	0.0	1.482796227469838E-5	0.0
9	2.5948933980722165E-4	0.0	0.0	2.2241943412047572E-5	0.0
10	2.7431730208192E-4	0.0	0.0	2.2241943412047572E-5	0.0
11	2.965592454939676E-4	0.0	0.0	2.2241943412047572E-5	0.0
12	2.965592454939676E-4	0.0	0.0	2.2241943412047572E-5	0.0
13	3.929410002795071E-4	0.0	0.0	2.2241943412047572E-5	0.0
14	4.3001090596625307E-4	0.0	0.0	2.2241943412047572E-5	0.0
15	4.967367362023957E-4	0.0	0.0	2.965592454939676E-5	0.0
16	5.4863460416384E-4	7.41398113734919E-6	0.0	2.965592454939676E-5	0.0
17	5.708765475758876E-4	7.41398113734919E-6	0.0	3.706990568674595E-5	0.0
18	6.969142269108239E-4	7.41398113734919E-6	0.0	3.706990568674595E-5	0.0
19	7.41398113734919E-4	7.41398113734919E-6	0.0	3.706990568674595E-5	0.0
20	8.007099628337125E-4	7.41398113734919E-6	0.0	3.706990568674595E-5	0.0
21	8.526078307951569E-4	7.41398113734919E-6	0.0	3.706990568674595E-5	0.0
22	9.119196798939505E-4	1.482796227469838E-5	0.0	3.706990568674595E-5	0.0
23	9.638175478553948E-4	1.482796227469838E-5	0.0	3.706990568674595E-5	0.0
24	0.001015715415816839	1.482796227469838E-5	0.0	3.706990568674595E-5	0.0
25	0.001089855227190331	1.482796227469838E-5	0.0	5.189786796144433E-5	7.41398113734919E-6
26	0.0011417530951517753	1.482796227469838E-5	0.0	5.189786796144433E-5	7.41398113734919E-6
27	0.0012677907744867115	1.482796227469838E-5	0.0	5.189786796144433E-5	1.482796227469838E-5
28	0.0013864144726842987	1.482796227469838E-5	0.0	5.931184909879352E-5	1.482796227469838E-5
29	0.001638489831354171	1.482796227469838E-5	0.0	5.931184909879352E-5	1.482796227469838E-5
30	0.001712629642727663	1.482796227469838E-5	0.0	5.931184909879352E-5	1.482796227469838E-5
31	0.0018831512088866944	1.482796227469838E-5	0.0	5.931184909879352E-5	1.482796227469838E-5
32	0.0020388448127710273	1.482796227469838E-5	0.0	8.155379251084109E-5	2.2241943412047572E-5
33	0.0022316083223421065	1.482796227469838E-5	0.0	8.155379251084109E-5	2.2241943412047572E-5
34	0.0024688557187372805	1.482796227469838E-5	0.0	8.155379251084109E-5	2.2241943412047572E-5
35	0.002698689133995105	2.2241943412047572E-5	0.0	9.638175478553947E-5	2.2241943412047572E-5
36	0.0029136945869782317	2.2241943412047572E-5	0.0	1.1120971706023786E-4	2.2241943412047572E-5
37	0.003188011889060152	2.2241943412047572E-5	0.0	1.2603767933493625E-4	2.2241943412047572E-5
38	0.003469743172279421	2.2241943412047572E-5	0.0	1.2603767933493625E-4	2.2241943412047572E-5
39	0.003781130380048087	2.965592454939676E-5	0.0	1.4086564160963462E-4	2.2241943412047572E-5
40	0.00402579175758061	2.965592454939676E-5	0.0	1.482796227469838E-4	2.2241943412047572E-5
41	0.004366834889898673	2.965592454939676E-5	0.0	1.55693603884333E-4	2.2241943412047572E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	17570	0.0	29.35828	1
TCAACGC	18840	0.0	27.341825	12
ATCAACG	19045	0.0	27.084538	11
CAACGCA	19020	0.0	27.05836	13
AGTGGTA	19400	0.0	26.467783	5
GGTATCA	19480	0.0	26.274641	8
AACGCAG	19750	0.0	26.141518	14
ACGCAGA	19885	0.0	25.881315	15
GTATCAA	19855	0.0	25.825735	9
GTGGTAT	19990	0.0	25.63957	6
TATCAAC	20120	0.0	25.532307	10
CGCAGAG	20420	0.0	25.203232	16
CTCGTAT	1390	0.0	24.683453	12
TGGTATC	20870	0.0	24.535936	7
AGAGTAC	20790	0.0	24.40428	19
GATCGGA	1415	0.0	24.24735	1
CAGTGGT	23005	0.0	22.401869	4
GCTCGTA	1540	0.0	22.279222	11
CAGAGTA	22940	0.0	22.270706	18
GCAGTGG	23580	0.0	22.005089	3
>>END_MODULE