FastQCFastQC Report
Thu 26 May 2016
SRR805287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18729447
Sequences flagged as poor quality0
Sequence length53
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1075090.5740105407276573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC520100.038.83983612
ATCAACG522850.038.65353411
CAACGCA522800.038.594313
GTGGTAT529950.037.9760326
GGTATCA531900.037.8589028
AAGCAGT531300.037.8531
GTATCAA533150.037.8362549
AGTGGTA532350.037.8268975
ACGCAGA535900.037.6464815
AACGCAG536550.037.61401714
TATCAAC540450.037.29910310
CGCAGAG541200.037.2604416
TGGTATC550100.036.6234327
AGAGTAC547200.036.3794819
CAGTGGT566700.035.5589374
CAGAGTA576200.034.70756518
GCAGTGG589850.034.4063723
GAGTACT225900.033.53873420
GTACATG366850.033.48357822
AGCAGTG609700.033.432672
Produced by FastQC (version 0.11.5)