##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805287_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18729447
Sequences flagged as poor quality	0
Sequence length	53
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.579349780054905	35.0	35.0	35.0	28.0	37.0
2	35.407250411611194	37.0	35.0	37.0	33.0	37.0
3	37.93191512808681	39.0	37.0	39.0	35.0	39.0
4	38.093175521946804	39.0	39.0	39.0	37.0	39.0
5	38.06538265651944	39.0	39.0	39.0	35.0	39.0
6	38.091592399925105	39.0	39.0	39.0	37.0	39.0
7	38.07844646988243	39.0	39.0	39.0	37.0	39.0
8	39.09557158841903	40.0	40.0	40.0	37.0	40.0
9	39.128636632998294	40.0	40.0	40.0	37.0	40.0
10	39.03579101935044	40.0	40.0	40.0	37.0	40.0
11	39.12153193844965	40.0	40.0	40.0	37.0	40.0
12	39.1357941854877	40.0	40.0	40.0	37.0	40.0
13	39.16797853134692	40.0	40.0	40.0	38.0	40.0
14	39.108944166904664	40.0	40.0	40.0	37.0	40.0
15	39.08826683457339	40.0	40.0	40.0	37.0	40.0
16	39.05366746813187	40.0	40.0	40.0	37.0	40.0
17	39.00698397555465	40.0	40.0	40.0	37.0	40.0
18	38.976070355948046	40.0	40.0	40.0	37.0	40.0
19	38.95781402408731	40.0	40.0	40.0	37.0	40.0
20	38.891775929102444	40.0	40.0	40.0	37.0	40.0
21	38.87105812574178	40.0	40.0	40.0	37.0	40.0
22	38.873911920624245	40.0	40.0	40.0	37.0	40.0
23	38.80543680761103	40.0	39.0	40.0	36.0	40.0
24	38.86586267069177	40.0	39.0	40.0	37.0	40.0
25	38.87825566873384	40.0	40.0	40.0	37.0	40.0
26	38.87705264335888	40.0	40.0	40.0	37.0	40.0
27	38.87473212636764	40.0	40.0	40.0	37.0	40.0
28	38.858930965767435	40.0	40.0	40.0	37.0	40.0
29	38.81343592258757	40.0	40.0	40.0	37.0	40.0
30	38.7654658463755	40.0	40.0	40.0	36.0	40.0
31	38.740867309109554	40.0	39.0	40.0	36.0	40.0
32	38.72457344843123	40.0	39.0	40.0	36.0	40.0
33	38.370402286837404	40.0	39.0	40.0	35.0	40.0
34	38.51123661045625	40.0	39.0	40.0	35.0	40.0
35	38.58717467739437	40.0	39.0	40.0	35.0	40.0
36	38.54997405956513	40.0	39.0	40.0	35.0	40.0
37	38.50398989356172	40.0	39.0	40.0	35.0	40.0
38	38.41858715849966	40.0	39.0	40.0	35.0	40.0
39	38.41158785948138	40.0	39.0	40.0	35.0	40.0
40	38.364807247112	40.0	38.0	40.0	35.0	40.0
41	38.34275470065934	40.0	38.0	40.0	35.0	40.0
42	38.27976891149002	40.0	38.0	40.0	35.0	40.0
43	38.20203730521248	40.0	38.0	40.0	35.0	40.0
44	38.07212919847554	40.0	38.0	40.0	34.0	40.0
45	37.161872905270506	40.0	37.0	40.0	32.0	40.0
46	37.59225363140727	40.0	37.0	40.0	33.0	40.0
47	37.711984395481615	40.0	37.0	40.0	33.0	40.0
48	37.613839639792886	40.0	37.0	40.0	33.0	40.0
49	37.67370948005032	40.0	37.0	40.0	34.0	40.0
50	37.654267261601476	40.0	37.0	40.0	34.0	40.0
51	37.71230325166568	40.0	37.0	40.0	34.0	40.0
52	37.641784405060115	40.0	37.0	40.0	34.0	40.0
53	37.190477860878644	40.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	2.0
12	15.0
13	57.0
14	149.0
15	469.0
16	1051.0
17	1767.0
18	2680.0
19	4065.0
20	5758.0
21	8368.0
22	11918.0
23	16967.0
24	23864.0
25	33815.0
26	46224.0
27	62075.0
28	82144.0
29	105326.0
30	133550.0
31	167548.0
32	209560.0
33	262897.0
34	342215.0
35	471781.0
36	699595.0
37	1258524.0
38	3123459.0
39	1.1653601E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.408427648718085	45.82519174218011	32.080028844418095	13.68635176468371
2	31.99619294686063	43.37450539783689	14.609774650580981	10.019527004721496
3	33.946928598585956	27.274612005362464	22.55161617959142	16.226843216460153
4	23.444552313797626	29.11271219059484	21.015340175286543	26.42739532032099
5	19.526321305695785	33.08851030145204	25.964076782405805	21.421091610446375
6	24.078543269323436	26.878150753730207	24.358209828619074	24.685096148327286
7	22.620940169776503	26.54094912679483	28.13415687072875	22.70395383269992
8	21.927460004558597	28.113969408707046	27.824521460777778	22.134049125956576
9	23.799138330138632	26.895113347446937	27.64152620202828	21.66422212038615
10	21.96497846412657	28.108443351263922	28.51088983032975	21.41568835427976
11	21.44022191365287	30.053578196943025	27.244183984716685	21.262015904687416
12	21.296608490362797	27.53645102281984	29.294682325644743	21.872258161172617
13	21.49711093979443	28.04617242569949	27.041668662187412	23.41504797231867
14	21.84940644536916	29.992775547510824	26.67435402657644	21.48346398054358
15	21.618299782155876	29.77715252351017	26.545209797171264	22.05933789716269
16	22.161636699684724	27.33336440739548	26.841086124966747	23.66391276795305
17	24.271042279037925	27.225059020696126	26.700291791850557	21.803606908415397
18	21.962276836043266	27.455733209848642	26.474422870039888	24.107567084068204
19	22.242146284404445	29.710749068031745	26.363944434664838	21.683160212898972
20	24.551942190284638	27.6251135444629	26.378130651695166	21.444813613557304
21	22.37681123206681	29.355175302292697	26.383384410655587	21.88462905498491
22	24.15903683648535	27.22621228485817	26.85225036275764	21.762500515898843
23	22.052941552412094	27.38963942715447	28.973151209429727	21.58426781100371
24	22.0728727335089	29.09621944524043	26.509293093383913	22.321614727866766
25	21.73463530450205	27.43789498963851	26.793823651066685	24.033646054792754
26	22.06444215891692	28.650931338229046	27.59129514074815	21.693331362105887
27	22.276616068803314	26.973353778144116	28.76370028437038	21.98632986868219
28	23.44720055002158	27.182452316931727	27.4917406797969	21.878606453249795
29	23.702926199582937	27.08037242103304	27.382356777538604	21.834344601845427
30	23.837996925376387	26.964661583441305	27.234226402947186	21.96311508823512
31	23.000369418274868	27.458627048625623	27.409234239537344	22.131769293562165
32	22.7332659634852	27.622497343354556	27.68567059134207	21.95856610181817
33	22.936640894950074	27.272930161792818	27.59070249110932	22.199726452147786
34	22.67450822226625	27.587397535015317	27.627788476616526	22.110305766101902
35	22.618457448316548	27.52498245143063	27.721736792335623	22.134823307917205
36	22.689335141608826	27.296977855245807	27.66852646530354	22.345160537841828
37	22.5595341923336	27.710764765238398	27.693284270485936	22.036416771942065
38	22.773587495669254	27.57129455023418	27.714181844237046	21.940936109859514
39	22.65929688153633	27.213051191527438	27.794168188735096	22.33348373820113
40	22.45228596444946	27.545057790547688	27.7698321792416	22.232824065761257
41	22.574590696671397	27.462786274469288	27.845728707313143	22.116894321546173
42	22.764553593066577	27.234274455620604	27.679397047867994	22.321774903444826
43	22.57868585228384	27.53006535644112	27.79869581840831	22.092552972866738
44	22.481000106409976	27.417744901918358	27.862002546044206	22.239252445627468
45	22.739897232416954	27.3749940401337	27.45385381639938	22.431254911049965
46	22.594932995085227	27.532831054755647	27.59644211598986	22.275793834169264
47	22.71767020136793	27.50137257122434	27.690427806010504	22.090529421397225
48	22.620881438731214	27.255567129130938	27.697689098882634	22.42586233325522
49	22.545662987273463	27.4438535211424	27.62465971365839	22.385823777925744
50	22.61609218894717	27.415812116609743	27.754097598290006	22.21399809615308
51	22.704722675474613	27.205154535528997	27.639417223583802	22.450705565412584
52	22.590608254477562	27.469203975963623	27.573515651583307	22.366672117975504
53	22.76655578779235	27.515772355692082	27.437814901849478	22.279856954666094
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1065.0
1	983.5
2	902.0
3	920.0
4	938.0
5	993.0
6	1048.0
7	1240.0
8	1948.5
9	2465.0
10	3668.5
11	4872.0
12	7086.0
13	9300.0
14	13867.0
15	18434.0
16	28003.5
17	37573.0
18	54234.5
19	70896.0
20	92655.5
21	114415.0
22	209517.0
23	304619.0
24	284577.0
25	292013.5
26	319492.0
27	357704.5
28	395917.0
29	445459.5
30	495002.0
31	548168.0
32	601334.0
33	657254.0
34	713174.0
35	779558.5
36	845943.0
37	907464.0
38	968985.0
39	1039921.5
40	1110858.0
41	1163654.0
42	1267192.0
43	1317934.0
44	1333092.5
45	1348251.0
46	1342429.5
47	1336608.0
48	1316345.5
49	1296083.0
50	1265857.5
51	1235632.0
52	1183985.5
53	1132339.0
54	1056160.5
55	979982.0
56	900674.0
57	821366.0
58	719609.0
59	528667.0
60	439482.0
61	367748.5
62	296015.0
63	241009.5
64	186004.0
65	147562.5
66	109121.0
67	83612.5
68	58104.0
69	43363.0
70	28622.0
71	21172.0
72	13722.0
73	10193.5
74	6665.0
75	4899.0
76	2202.0
77	1271.0
78	975.5
79	680.0
80	531.0
81	382.0
82	300.0
83	218.0
84	166.5
85	115.0
86	97.5
87	80.0
88	65.0
89	50.0
90	42.5
91	35.0
92	26.5
93	15.5
94	13.0
95	12.0
96	11.0
97	10.0
98	9.0
99	5.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.8729447E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.843205531489744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.843521925314	36.01911745823339
2	15.875459760432872	16.143185270131667
3	5.430422065218949	8.28300195553992
4	2.467998699665056	5.019238605540795
5	1.3739310854551097	3.492753028194847
6	0.8576774131943087	2.6164241399252814
7	0.6170250463124933	2.1960071873420155
8	0.4241842622483175	1.7253510102971645
9	0.3269310363048193	1.4959999686121976
>10	1.6413764429555906	14.819211325587473
>50	0.0999897088996385	3.4811781770353956
>100	0.04010194740537886	3.4678964509345995
>500	0.0010753902343867401	0.35511321491060727
>1k	2.7366162246335096E-4	0.24673127332598657
>5k	2.1036490937593807E-5	0.06385288915436113
>10k+	1.0518245468794081E-5	0.5749380452340581
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107509	0.5740105407276573	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0678371870776538E-4	5.339185935388269E-6	0.0	0.0	0.0
2	6.460414981819805E-4	5.339185935388269E-6	0.0	0.0	0.0
3	6.460414981819805E-4	5.339185935388269E-6	0.0	5.339185935388269E-6	0.0
4	6.727374278589219E-4	5.339185935388269E-6	0.0	5.339185935388269E-6	5.339185935388269E-6
5	6.834157997296984E-4	5.339185935388269E-6	0.0	5.339185935388269E-6	5.339185935388269E-6
6	6.94094171600475E-4	5.339185935388269E-6	0.0	5.339185935388269E-6	5.339185935388269E-6
7	7.047725434712514E-4	5.339185935388269E-6	0.0	1.0678371870776538E-5	5.339185935388269E-6
8	7.154509153420279E-4	5.339185935388269E-6	0.0	1.6017557806164807E-5	5.339185935388269E-6
9	7.36807659083581E-4	5.339185935388269E-6	0.0	3.203511561232961E-5	5.339185935388269E-6
10	7.528252168897459E-4	1.0678371870776538E-5	0.0	3.737430154771788E-5	1.6017557806164807E-5
11	7.848603325020754E-4	1.0678371870776538E-5	0.0	4.271348748310615E-5	1.6017557806164807E-5
12	8.115562621790168E-4	1.0678371870776538E-5	0.0	4.271348748310615E-5	1.6017557806164807E-5
13	9.770710261760532E-4	1.0678371870776538E-5	0.0	4.271348748310615E-5	1.6017557806164807E-5
14	0.001035802071465324	1.0678371870776538E-5	0.0	4.271348748310615E-5	1.6017557806164807E-5
15	0.0012280127651393019	1.0678371870776538E-5	0.0	4.271348748310615E-5	1.6017557806164807E-5
16	0.0012493695088808547	1.6017557806164807E-5	0.0	4.271348748310615E-5	1.6017557806164807E-5
17	0.0012600478807516314	2.1356743741553075E-5	0.0	4.271348748310615E-5	1.6017557806164807E-5
18	0.0014362410166194443	2.1356743741553075E-5	0.0	4.8052673418494416E-5	1.6017557806164807E-5
19	0.001452258574425609	2.1356743741553075E-5	0.0	5.8731045289270954E-5	1.6017557806164807E-5
20	0.0014949720619087151	2.1356743741553075E-5	0.0	5.8731045289270954E-5	1.6017557806164807E-5
21	0.0015270071775210449	2.1356743741553075E-5	0.0	5.8731045289270954E-5	1.6017557806164807E-5
22	0.0015857382228103157	2.1356743741553075E-5	0.0	5.8731045289270954E-5	1.6017557806164807E-5
23	0.0016604868259057514	2.1356743741553075E-5	0.0	5.8731045289270954E-5	1.6017557806164807E-5
24	0.0017032003133888576	2.1356743741553075E-5	0.0	5.8731045289270954E-5	1.6017557806164807E-5
25	0.0017832881024196817	2.1356743741553075E-5	0.0	5.8731045289270954E-5	1.6017557806164807E-5
26	0.0019060893789336118	3.203511561232961E-5	0.0	6.407023122465923E-5	1.6017557806164807E-5
27	0.0022157621631861313	3.203511561232961E-5	0.0	6.407023122465923E-5	1.6017557806164807E-5
28	0.0022905107662815672	3.203511561232961E-5	0.0	6.940941716004748E-5	1.6017557806164807E-5
29	0.0028991779629158297	3.203511561232961E-5	0.0	7.474860309543576E-5	1.6017557806164807E-5
30	0.003048675169106701	3.203511561232961E-5	0.0	8.008778903082403E-5	1.6017557806164807E-5
31	0.0034971667876793158	3.737430154771788E-5	0.0	8.008778903082403E-5	1.6017557806164807E-5
32	0.0036039505063870814	4.8052673418494416E-5	0.0	8.008778903082403E-5	1.6017557806164807E-5
33	0.0037481085266425646	4.8052673418494416E-5	0.0	8.008778903082403E-5	1.6017557806164807E-5
34	0.0038281963156733883	5.339185935388269E-5	0.0	8.54269749662123E-5	1.6017557806164807E-5
35	0.0039723543359288715	5.8731045289270954E-5	0.0	8.54269749662123E-5	1.6017557806164807E-5
36	0.004228635260827509	5.8731045289270954E-5	0.0	8.54269749662123E-5	1.6017557806164807E-5
37	0.004693144437206288	5.8731045289270954E-5	0.0	8.54269749662123E-5	1.6017557806164807E-5
38	0.005008156407394196	5.8731045289270954E-5	5.339185935388269E-6	8.54269749662123E-5	1.6017557806164807E-5
39	0.005146975241714291	5.8731045289270954E-5	5.339185935388269E-6	8.54269749662123E-5	1.6017557806164807E-5
40	0.005291133261969774	5.8731045289270954E-5	5.339185935388269E-6	8.54269749662123E-5	1.6017557806164807E-5
41	0.005494022327514528	6.940941716004748E-5	5.339185935388269E-6	9.076616090160056E-5	1.6017557806164807E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	52010	0.0	38.839836	12
ATCAACG	52285	0.0	38.653534	11
CAACGCA	52280	0.0	38.5943	13
GTGGTAT	52995	0.0	37.976032	6
GGTATCA	53190	0.0	37.858902	8
AAGCAGT	53130	0.0	37.853	1
GTATCAA	53315	0.0	37.836254	9
AGTGGTA	53235	0.0	37.826897	5
ACGCAGA	53590	0.0	37.64648	15
AACGCAG	53655	0.0	37.614017	14
TATCAAC	54045	0.0	37.299103	10
CGCAGAG	54120	0.0	37.26044	16
TGGTATC	55010	0.0	36.623432	7
AGAGTAC	54720	0.0	36.37948	19
CAGTGGT	56670	0.0	35.558937	4
CAGAGTA	57620	0.0	34.707565	18
GCAGTGG	58985	0.0	34.406372	3
GAGTACT	22590	0.0	33.538734	20
GTACATG	36685	0.0	33.483578	22
AGCAGTG	60970	0.0	33.43267	2
>>END_MODULE